Experiment: Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS21920 and HSERO_RS21925 are separated by 110 nucleotides HSERO_RS21925 and HSERO_RS21930 are separated by 78 nucleotides HSERO_RS21930 and HSERO_RS21935 are separated by 233 nucleotides HSERO_RS21935 and HSERO_RS21940 are separated by 103 nucleotides HSERO_RS21940 and HSERO_RS21945 are separated by 159 nucleotides
HSERO_RS21920: HSERO_RS21920 - iron ABC transporter substrate-binding protein, at 5,018,154 to 5,019,242
_RS21920
HSERO_RS21925: HSERO_RS21925 - hypothetical protein, at 5,019,353 to 5,019,673
_RS21925
HSERO_RS21930: HSERO_RS21930 - tRNA-Arg, at 5,019,752 to 5,019,827
_RS21930
HSERO_RS21935: HSERO_RS21935 - cytochrome C, at 5,020,061 to 5,020,630
_RS21935
HSERO_RS21940: HSERO_RS21940 - membrane protein, at 5,020,734 to 5,021,459
_RS21940
HSERO_RS21945: HSERO_RS21945 - hypothetical protein, at 5,021,619 to 5,022,050
_RS21945
Position (kb)
5020
5021 Strain fitness (log2 ratio)
-1
0
1 at 5019.085 kb on - strand, within HSERO_RS21920 at 5019.224 kb on + strand at 5019.224 kb on + strand at 5019.347 kb on - strand at 5019.518 kb on + strand, within HSERO_RS21925 at 5019.689 kb on + strand at 5019.890 kb on - strand at 5020.016 kb on - strand at 5020.050 kb on - strand at 5020.065 kb on + strand at 5020.073 kb on - strand at 5020.073 kb on - strand at 5020.083 kb on - strand at 5020.229 kb on - strand, within HSERO_RS21935 at 5020.422 kb on - strand, within HSERO_RS21935 at 5020.622 kb on + strand at 5020.721 kb on + strand at 5021.030 kb on - strand, within HSERO_RS21940
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB remove 5,019,085 - HSERO_RS21920 0.85 +0.4 5,019,224 + -0.6 5,019,224 + -0.4 5,019,347 - +0.9 5,019,518 + HSERO_RS21925 0.51 +0.6 5,019,689 + -0.4 5,019,890 - -0.5 5,020,016 - +1.4 5,020,050 - -0.7 5,020,065 + +0.1 5,020,073 - +0.8 5,020,073 - -0.7 5,020,083 - +0.3 5,020,229 - HSERO_RS21935 0.29 +0.2 5,020,422 - HSERO_RS21935 0.63 +0.6 5,020,622 + -0.8 5,020,721 + -0.5 5,021,030 - HSERO_RS21940 0.41 -0.3
Or see this region's nucleotide sequence