Experiment: Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS21645 and HSERO_RS21650 are separated by 78 nucleotides HSERO_RS21650 and HSERO_RS21655 are separated by 250 nucleotides HSERO_RS21655 and HSERO_RS21660 are separated by 207 nucleotides HSERO_RS21660 and HSERO_RS21665 are separated by 110 nucleotides
HSERO_RS21645: HSERO_RS21645 - hydroxyglutarate oxidase, at 4,955,150 to 4,956,358
_RS21645
HSERO_RS21650: HSERO_RS21650 - TetR family transcriptional regulator, at 4,956,437 to 4,957,069
_RS21650
HSERO_RS21655: HSERO_RS21655 - elongation factor G, at 4,957,320 to 4,959,425
_RS21655
HSERO_RS21660: HSERO_RS21660 - tRNA-Asp, at 4,959,633 to 4,959,709
_RS21660
HSERO_RS21665: HSERO_RS21665 - oxygen-insensitive nitroreductase, at 4,959,820 to 4,960,413
_RS21665
Position (kb) 4957
4958
4959
4960 Strain fitness (log2 ratio) -1
0
1
2
3 at 4956.393 kb on + strand at 4956.419 kb on - strand at 4956.459 kb on + strand at 4956.520 kb on + strand, within HSERO_RS21650 at 4956.697 kb on - strand, within HSERO_RS21650 at 4956.801 kb on + strand, within HSERO_RS21650 at 4957.094 kb on - strand at 4957.446 kb on + strand at 4957.448 kb on + strand at 4957.661 kb on - strand, within HSERO_RS21655 at 4957.693 kb on - strand, within HSERO_RS21655 at 4958.177 kb on + strand, within HSERO_RS21655 at 4958.185 kb on - strand, within HSERO_RS21655 at 4958.481 kb on + strand, within HSERO_RS21655 at 4958.504 kb on + strand, within HSERO_RS21655 at 4958.623 kb on + strand, within HSERO_RS21655 at 4958.940 kb on - strand, within HSERO_RS21655 at 4958.973 kb on + strand, within HSERO_RS21655 at 4959.028 kb on + strand, within HSERO_RS21655 at 4959.231 kb on - strand at 4959.264 kb on - strand at 4959.387 kb on + strand at 4959.422 kb on - strand at 4959.473 kb on + strand at 4959.501 kb on + strand at 4959.572 kb on - strand at 4959.577 kb on - strand at 4959.857 kb on - strand at 4959.925 kb on - strand, within HSERO_RS21665 at 4960.019 kb on - strand, within HSERO_RS21665 at 4960.065 kb on - strand, within HSERO_RS21665 at 4960.082 kb on - strand, within HSERO_RS21665
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB remove 4,956,393 + +0.1 4,956,419 - -0.2 4,956,459 + -0.8 4,956,520 + HSERO_RS21650 0.13 +0.0 4,956,697 - HSERO_RS21650 0.41 -1.7 4,956,801 + HSERO_RS21650 0.58 -0.8 4,957,094 - +1.9 4,957,446 + -0.1 4,957,448 + +0.8 4,957,661 - HSERO_RS21655 0.16 +2.2 4,957,693 - HSERO_RS21655 0.18 +3.5 4,958,177 + HSERO_RS21655 0.41 +2.2 4,958,185 - HSERO_RS21655 0.41 +1.6 4,958,481 + HSERO_RS21655 0.55 +0.5 4,958,504 + HSERO_RS21655 0.56 +0.2 4,958,623 + HSERO_RS21655 0.62 +0.7 4,958,940 - HSERO_RS21655 0.77 -1.4 4,958,973 + HSERO_RS21655 0.78 +0.5 4,959,028 + HSERO_RS21655 0.81 +0.3 4,959,231 - +0.5 4,959,264 - -0.1 4,959,387 + -0.8 4,959,422 - -0.9 4,959,473 + -0.4 4,959,501 + -0.1 4,959,572 - +1.0 4,959,577 - +1.0 4,959,857 - -0.3 4,959,925 - HSERO_RS21665 0.18 -1.4 4,960,019 - HSERO_RS21665 0.34 -0.0 4,960,065 - HSERO_RS21665 0.41 +0.0 4,960,082 - HSERO_RS21665 0.44 -0.5
Or see this region's nucleotide sequence