Strain Fitness in Herbaspirillum seropedicae SmR1 around HSERO_RS20250

Experiment: Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntHSERO_RS20240 and HSERO_RS20245 are separated by 2 nucleotidesHSERO_RS20245 and HSERO_RS20250 are separated by 292 nucleotidesHSERO_RS20250 and HSERO_RS20255 are separated by 13 nucleotidesHSERO_RS20255 and HSERO_RS20260 are separated by 43 nucleotides HSERO_RS20240: HSERO_RS20240 - cytochrome B, at 4,644,426 to 4,645,832 _RS20240 HSERO_RS20245: HSERO_RS20245 - ubiquinol-cytochrome C reductase, at 4,645,835 to 4,646,443 _RS20245 HSERO_RS20250: HSERO_RS20250 - large conductance mechanosensitive channel protein MscL, at 4,646,736 to 4,647,173 _RS20250 HSERO_RS20255: HSERO_RS20255 - metal-binding protein, at 4,647,187 to 4,647,951 _RS20255 HSERO_RS20260: HSERO_RS20260 - 2-alkenal reductase, at 4,647,995 to 4,649,176 _RS20260 Position (kb) 4646 4647 4648Strain fitness (log2 ratio) -1 0 1 2at 4645.828 kb on + strandat 4646.447 kb on - strandat 4646.447 kb on - strandat 4646.449 kb on + strandat 4646.479 kb on + strandat 4646.487 kb on - strandat 4646.487 kb on - strandat 4646.549 kb on + strandat 4646.554 kb on + strandat 4646.586 kb on + strandat 4646.600 kb on - strandat 4646.622 kb on + strandat 4646.622 kb on + strandat 4646.675 kb on + strandat 4646.681 kb on - strandat 4646.682 kb on - strandat 4646.683 kb on - strandat 4646.887 kb on + strand, within HSERO_RS20250at 4647.167 kb on + strandat 4647.174 kb on + strandat 4647.174 kb on + strandat 4647.182 kb on - strandat 4647.182 kb on - strandat 4647.657 kb on - strand, within HSERO_RS20255at 4647.710 kb on - strand, within HSERO_RS20255at 4647.788 kb on + strand, within HSERO_RS20255at 4647.833 kb on - strand, within HSERO_RS20255at 4647.855 kb on - strand, within HSERO_RS20255at 4647.917 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB
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4,645,828 + -1.3
4,646,447 - -0.4
4,646,447 - +0.2
4,646,449 + -1.6
4,646,479 + -0.6
4,646,487 - -1.4
4,646,487 - -0.7
4,646,549 + +2.5
4,646,554 + -0.8
4,646,586 + +0.6
4,646,600 - -0.1
4,646,622 + -0.3
4,646,622 + +0.6
4,646,675 + +0.6
4,646,681 - -0.1
4,646,682 - -1.2
4,646,683 - -0.9
4,646,887 + HSERO_RS20250 0.34 +0.6
4,647,167 + +0.3
4,647,174 + -0.2
4,647,174 + -0.0
4,647,182 - +1.3
4,647,182 - -0.3
4,647,657 - HSERO_RS20255 0.61 -0.4
4,647,710 - HSERO_RS20255 0.68 +0.9
4,647,788 + HSERO_RS20255 0.79 +0.3
4,647,833 - HSERO_RS20255 0.84 -0.6
4,647,855 - HSERO_RS20255 0.87 -0.3
4,647,917 + -0.0

Or see this region's nucleotide sequence