Experiment: Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS18785 and HSERO_RS18790 are separated by 280 nucleotides HSERO_RS18790 and HSERO_RS18795 overlap by 4 nucleotides HSERO_RS18795 and HSERO_RS18800 are separated by 4 nucleotides
HSERO_RS18785: HSERO_RS18785 - polymerase, at 4,310,257 to 4,311,399
_RS18785
HSERO_RS18790: HSERO_RS18790 - xylose isomerase, at 4,311,680 to 4,312,552
_RS18790
HSERO_RS18795: HSERO_RS18795 - shikimate dehydrogenase, at 4,312,549 to 4,313,394
_RS18795
HSERO_RS18800: HSERO_RS18800 - 4-hydroxyphenylpyruvate dioxygenase, at 4,313,399 to 4,314,280
_RS18800
Position (kb) 4311
4312
4313 Strain fitness (log2 ratio) -1
0
1 at 4310.682 kb on - strand, within HSERO_RS18785 at 4310.723 kb on + strand, within HSERO_RS18785 at 4310.963 kb on + strand, within HSERO_RS18785 at 4310.985 kb on - strand, within HSERO_RS18785 at 4311.004 kb on - strand, within HSERO_RS18785 at 4311.042 kb on - strand, within HSERO_RS18785 at 4311.091 kb on - strand, within HSERO_RS18785 at 4311.121 kb on - strand, within HSERO_RS18785 at 4311.243 kb on + strand, within HSERO_RS18785 at 4311.304 kb on - strand at 4311.306 kb on + strand at 4311.423 kb on + strand at 4311.464 kb on - strand at 4311.504 kb on + strand at 4311.524 kb on + strand at 4311.532 kb on - strand at 4311.575 kb on + strand at 4311.583 kb on - strand at 4311.583 kb on - strand at 4311.815 kb on + strand, within HSERO_RS18790 at 4312.128 kb on + strand, within HSERO_RS18790 at 4312.128 kb on + strand, within HSERO_RS18790 at 4312.428 kb on - strand, within HSERO_RS18790 at 4312.613 kb on - strand at 4312.738 kb on + strand, within HSERO_RS18795 at 4313.007 kb on - strand, within HSERO_RS18795 at 4313.160 kb on + strand, within HSERO_RS18795 at 4313.243 kb on + strand, within HSERO_RS18795 at 4313.320 kb on + strand at 4313.327 kb on + strand at 4313.327 kb on + strand at 4313.335 kb on - strand at 4313.358 kb on - strand at 4313.488 kb on - strand, within HSERO_RS18800 at 4313.540 kb on + strand, within HSERO_RS18800
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB remove 4,310,682 - HSERO_RS18785 0.37 +0.5 4,310,723 + HSERO_RS18785 0.41 -0.7 4,310,963 + HSERO_RS18785 0.62 +0.3 4,310,985 - HSERO_RS18785 0.64 +1.3 4,311,004 - HSERO_RS18785 0.65 -0.1 4,311,042 - HSERO_RS18785 0.69 +0.6 4,311,091 - HSERO_RS18785 0.73 -0.8 4,311,121 - HSERO_RS18785 0.76 +0.3 4,311,243 + HSERO_RS18785 0.86 +0.2 4,311,304 - +0.4 4,311,306 + +1.2 4,311,423 + +1.0 4,311,464 - -1.4 4,311,504 + +0.7 4,311,524 + +0.4 4,311,532 - -0.1 4,311,575 + -0.0 4,311,583 - -0.6 4,311,583 - +0.3 4,311,815 + HSERO_RS18790 0.15 +0.5 4,312,128 + HSERO_RS18790 0.51 +0.2 4,312,128 + HSERO_RS18790 0.51 -1.2 4,312,428 - HSERO_RS18790 0.86 +1.7 4,312,613 - -0.4 4,312,738 + HSERO_RS18795 0.22 +0.2 4,313,007 - HSERO_RS18795 0.54 +0.6 4,313,160 + HSERO_RS18795 0.72 -0.7 4,313,243 + HSERO_RS18795 0.82 -0.1 4,313,320 + -0.1 4,313,327 + +0.6 4,313,327 + -0.3 4,313,335 - -0.4 4,313,358 - -0.8 4,313,488 - HSERO_RS18800 0.10 -0.1 4,313,540 + HSERO_RS18800 0.16 +0.3
Or see this region's nucleotide sequence