Experiment: Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS18240 and HSERO_RS18245 are separated by 26 nucleotides HSERO_RS18245 and HSERO_RS18250 are separated by 108 nucleotides HSERO_RS18250 and HSERO_RS18255 overlap by 4 nucleotides HSERO_RS18255 and HSERO_RS18260 overlap by 11 nucleotides
HSERO_RS18240: HSERO_RS18240 - riboflavin synthase subunit alpha, at 4,187,700 to 4,188,320
_RS18240
HSERO_RS18245: HSERO_RS18245 - lipase, at 4,188,347 to 4,189,195
_RS18245
HSERO_RS18250: HSERO_RS18250 - ABC transporter ATP-binding protein, at 4,189,304 to 4,190,014
_RS18250
HSERO_RS18255: HSERO_RS18255 - branched-chain amino acid ABC transporter substrate-binding protein, at 4,190,011 to 4,190,778
_RS18255
HSERO_RS18260: HSERO_RS18260 - branched-chain amino acid ABC transporter permease, at 4,190,768 to 4,191,721
_RS18260
Position (kb) 4189
4190
4191 Strain fitness (log2 ratio) -2
-1
0
1 at 4188.346 kb on - strand at 4188.355 kb on + strand at 4188.355 kb on + strand at 4188.365 kb on + strand at 4188.396 kb on + strand at 4188.437 kb on - strand, within HSERO_RS18245 at 4188.556 kb on + strand, within HSERO_RS18245 at 4188.696 kb on + strand, within HSERO_RS18245 at 4188.816 kb on + strand, within HSERO_RS18245 at 4188.922 kb on - strand, within HSERO_RS18245 at 4189.884 kb on - strand, within HSERO_RS18250 at 4190.139 kb on - strand, within HSERO_RS18255 at 4190.257 kb on - strand, within HSERO_RS18255 at 4190.300 kb on - strand, within HSERO_RS18255 at 4190.398 kb on + strand, within HSERO_RS18255 at 4190.934 kb on + strand, within HSERO_RS18260 at 4190.942 kb on - strand, within HSERO_RS18260 at 4190.972 kb on - strand, within HSERO_RS18260 at 4191.011 kb on + strand, within HSERO_RS18260
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB remove 4,188,346 - +0.8 4,188,355 + -0.5 4,188,355 + +0.5 4,188,365 + -1.8 4,188,396 + -0.1 4,188,437 - HSERO_RS18245 0.11 -0.2 4,188,556 + HSERO_RS18245 0.25 -0.6 4,188,696 + HSERO_RS18245 0.41 -0.9 4,188,816 + HSERO_RS18245 0.55 +0.7 4,188,922 - HSERO_RS18245 0.68 +0.3 4,189,884 - HSERO_RS18250 0.82 +0.4 4,190,139 - HSERO_RS18255 0.17 +1.7 4,190,257 - HSERO_RS18255 0.32 -0.7 4,190,300 - HSERO_RS18255 0.38 -0.4 4,190,398 + HSERO_RS18255 0.50 +0.6 4,190,934 + HSERO_RS18260 0.17 +0.2 4,190,942 - HSERO_RS18260 0.18 +0.2 4,190,972 - HSERO_RS18260 0.21 +0.2 4,191,011 + HSERO_RS18260 0.25 +1.4
Or see this region's nucleotide sequence