Strain Fitness in Herbaspirillum seropedicae SmR1 around HSERO_RS14200

Experiment: Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntHSERO_RS14185 and HSERO_RS14190 are separated by 43 nucleotidesHSERO_RS14190 and HSERO_RS14195 are separated by 12 nucleotidesHSERO_RS14195 and HSERO_RS14200 are separated by 3 nucleotidesHSERO_RS14200 and HSERO_RS14205 are separated by 51 nucleotidesHSERO_RS14205 and HSERO_RS14210 are separated by 209 nucleotides HSERO_RS14185: HSERO_RS14185 - FeS cluster assembly scaffold IscU, at 3,226,489 to 3,226,938 _RS14185 HSERO_RS14190: HSERO_RS14190 - iron--sulfur cluster insertion protein ErpA, at 3,226,982 to 3,227,338 _RS14190 HSERO_RS14195: HSERO_RS14195 - heme biosynthesis protein HemY, at 3,227,351 to 3,227,671 _RS14195 HSERO_RS14200: HSERO_RS14200 - molecular chaperone HscA, at 3,227,675 to 3,229,603 _RS14200 HSERO_RS14205: HSERO_RS14205 - ferredoxin [2Fe-2S]-type protein, at 3,229,655 to 3,229,993 _RS14205 HSERO_RS14210: HSERO_RS14210 - homocitrate synthase, at 3,230,203 to 3,231,333 _RS14210 Position (kb) 3227 3228 3229 3230Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3226.813 kb on - strand, within HSERO_RS14185at 3227.131 kb on - strand, within HSERO_RS14190at 3227.561 kb on - strand, within HSERO_RS14195at 3227.673 kb on + strandat 3227.835 kb on + strandat 3227.923 kb on + strand, within HSERO_RS14200at 3227.943 kb on - strand, within HSERO_RS14200at 3227.996 kb on + strand, within HSERO_RS14200at 3228.014 kb on + strand, within HSERO_RS14200at 3228.016 kb on - strand, within HSERO_RS14200at 3228.016 kb on - strand, within HSERO_RS14200at 3228.201 kb on + strand, within HSERO_RS14200at 3228.518 kb on - strandat 3228.571 kb on - strand, within HSERO_RS14200at 3228.645 kb on + strand, within HSERO_RS14200at 3228.653 kb on - strand, within HSERO_RS14200at 3228.686 kb on + strand, within HSERO_RS14200at 3228.686 kb on + strand, within HSERO_RS14200at 3228.686 kb on + strand, within HSERO_RS14200at 3228.993 kb on + strand, within HSERO_RS14200at 3229.229 kb on - strand, within HSERO_RS14200at 3229.274 kb on - strand, within HSERO_RS14200at 3229.417 kb on + strandat 3229.425 kb on - strandat 3229.427 kb on - strandat 3229.561 kb on + strandat 3229.569 kb on - strandat 3229.680 kb on + strandat 3229.724 kb on + strandat 3229.777 kb on - strand, within HSERO_RS14205at 3229.931 kb on + strand, within HSERO_RS14205at 3229.957 kb on + strand, within HSERO_RS14205at 3229.965 kb on - strandat 3229.965 kb on - strandat 3230.059 kb on + strandat 3230.067 kb on - strandat 3230.067 kb on - strandat 3230.219 kb on + strandat 3230.227 kb on - strandat 3230.227 kb on - strandat 3230.227 kb on - strandat 3230.237 kb on + strandat 3230.254 kb on - strandat 3230.370 kb on + strand, within HSERO_RS14210

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Per-strain Table

Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB
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3,226,813 - HSERO_RS14185 0.72 -0.8
3,227,131 - HSERO_RS14190 0.42 +0.0
3,227,561 - HSERO_RS14195 0.65 +0.7
3,227,673 + +1.3
3,227,835 + -0.2
3,227,923 + HSERO_RS14200 0.13 +1.9
3,227,943 - HSERO_RS14200 0.14 +1.6
3,227,996 + HSERO_RS14200 0.17 -0.0
3,228,014 + HSERO_RS14200 0.18 -0.1
3,228,016 - HSERO_RS14200 0.18 +0.7
3,228,016 - HSERO_RS14200 0.18 +1.8
3,228,201 + HSERO_RS14200 0.27 +0.5
3,228,518 - +3.3
3,228,571 - HSERO_RS14200 0.46 -0.0
3,228,645 + HSERO_RS14200 0.50 +0.9
3,228,653 - HSERO_RS14200 0.51 +0.5
3,228,686 + HSERO_RS14200 0.52 +0.0
3,228,686 + HSERO_RS14200 0.52 +0.4
3,228,686 + HSERO_RS14200 0.52 +0.9
3,228,993 + HSERO_RS14200 0.68 +0.1
3,229,229 - HSERO_RS14200 0.81 -0.5
3,229,274 - HSERO_RS14200 0.83 +0.1
3,229,417 + +0.3
3,229,425 - -0.9
3,229,427 - +0.1
3,229,561 + -0.4
3,229,569 - -1.7
3,229,680 + -0.1
3,229,724 + +2.1
3,229,777 - HSERO_RS14205 0.36 +0.1
3,229,931 + HSERO_RS14205 0.81 -0.4
3,229,957 + HSERO_RS14205 0.89 +0.0
3,229,965 - -0.7
3,229,965 - +0.8
3,230,059 + -2.0
3,230,067 - +0.0
3,230,067 - +0.1
3,230,219 + +0.4
3,230,227 - +2.1
3,230,227 - -0.1
3,230,227 - +1.9
3,230,237 + -0.2
3,230,254 - -0.5
3,230,370 + HSERO_RS14210 0.15 -0.8

Or see this region's nucleotide sequence