Experiment: Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS14185 and HSERO_RS14190 are separated by 43 nucleotides HSERO_RS14190 and HSERO_RS14195 are separated by 12 nucleotides HSERO_RS14195 and HSERO_RS14200 are separated by 3 nucleotides HSERO_RS14200 and HSERO_RS14205 are separated by 51 nucleotides HSERO_RS14205 and HSERO_RS14210 are separated by 209 nucleotides
HSERO_RS14185: HSERO_RS14185 - FeS cluster assembly scaffold IscU, at 3,226,489 to 3,226,938
_RS14185
HSERO_RS14190: HSERO_RS14190 - iron--sulfur cluster insertion protein ErpA, at 3,226,982 to 3,227,338
_RS14190
HSERO_RS14195: HSERO_RS14195 - heme biosynthesis protein HemY, at 3,227,351 to 3,227,671
_RS14195
HSERO_RS14200: HSERO_RS14200 - molecular chaperone HscA, at 3,227,675 to 3,229,603
_RS14200
HSERO_RS14205: HSERO_RS14205 - ferredoxin [2Fe-2S]-type protein, at 3,229,655 to 3,229,993
_RS14205
HSERO_RS14210: HSERO_RS14210 - homocitrate synthase, at 3,230,203 to 3,231,333
_RS14210
Position (kb)
3227
3228
3229
3230 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3226.813 kb on - strand, within HSERO_RS14185 at 3227.131 kb on - strand, within HSERO_RS14190 at 3227.561 kb on - strand, within HSERO_RS14195 at 3227.673 kb on + strand at 3227.835 kb on + strand at 3227.923 kb on + strand, within HSERO_RS14200 at 3227.943 kb on - strand, within HSERO_RS14200 at 3227.996 kb on + strand, within HSERO_RS14200 at 3228.014 kb on + strand, within HSERO_RS14200 at 3228.016 kb on - strand, within HSERO_RS14200 at 3228.016 kb on - strand, within HSERO_RS14200 at 3228.201 kb on + strand, within HSERO_RS14200 at 3228.518 kb on - strand at 3228.571 kb on - strand, within HSERO_RS14200 at 3228.645 kb on + strand, within HSERO_RS14200 at 3228.653 kb on - strand, within HSERO_RS14200 at 3228.686 kb on + strand, within HSERO_RS14200 at 3228.686 kb on + strand, within HSERO_RS14200 at 3228.686 kb on + strand, within HSERO_RS14200 at 3228.993 kb on + strand, within HSERO_RS14200 at 3229.229 kb on - strand, within HSERO_RS14200 at 3229.274 kb on - strand, within HSERO_RS14200 at 3229.417 kb on + strand at 3229.425 kb on - strand at 3229.427 kb on - strand at 3229.561 kb on + strand at 3229.569 kb on - strand at 3229.680 kb on + strand at 3229.724 kb on + strand at 3229.777 kb on - strand, within HSERO_RS14205 at 3229.931 kb on + strand, within HSERO_RS14205 at 3229.957 kb on + strand, within HSERO_RS14205 at 3229.965 kb on - strand at 3229.965 kb on - strand at 3230.059 kb on + strand at 3230.067 kb on - strand at 3230.067 kb on - strand at 3230.219 kb on + strand at 3230.227 kb on - strand at 3230.227 kb on - strand at 3230.227 kb on - strand at 3230.237 kb on + strand at 3230.254 kb on - strand at 3230.370 kb on + strand, within HSERO_RS14210
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB remove 3,226,813 - HSERO_RS14185 0.72 -0.8 3,227,131 - HSERO_RS14190 0.42 +0.0 3,227,561 - HSERO_RS14195 0.65 +0.7 3,227,673 + +1.3 3,227,835 + -0.2 3,227,923 + HSERO_RS14200 0.13 +1.9 3,227,943 - HSERO_RS14200 0.14 +1.6 3,227,996 + HSERO_RS14200 0.17 -0.0 3,228,014 + HSERO_RS14200 0.18 -0.1 3,228,016 - HSERO_RS14200 0.18 +0.7 3,228,016 - HSERO_RS14200 0.18 +1.8 3,228,201 + HSERO_RS14200 0.27 +0.5 3,228,518 - +3.3 3,228,571 - HSERO_RS14200 0.46 -0.0 3,228,645 + HSERO_RS14200 0.50 +0.9 3,228,653 - HSERO_RS14200 0.51 +0.5 3,228,686 + HSERO_RS14200 0.52 +0.0 3,228,686 + HSERO_RS14200 0.52 +0.4 3,228,686 + HSERO_RS14200 0.52 +0.9 3,228,993 + HSERO_RS14200 0.68 +0.1 3,229,229 - HSERO_RS14200 0.81 -0.5 3,229,274 - HSERO_RS14200 0.83 +0.1 3,229,417 + +0.3 3,229,425 - -0.9 3,229,427 - +0.1 3,229,561 + -0.4 3,229,569 - -1.7 3,229,680 + -0.1 3,229,724 + +2.1 3,229,777 - HSERO_RS14205 0.36 +0.1 3,229,931 + HSERO_RS14205 0.81 -0.4 3,229,957 + HSERO_RS14205 0.89 +0.0 3,229,965 - -0.7 3,229,965 - +0.8 3,230,059 + -2.0 3,230,067 - +0.0 3,230,067 - +0.1 3,230,219 + +0.4 3,230,227 - +2.1 3,230,227 - -0.1 3,230,227 - +1.9 3,230,237 + -0.2 3,230,254 - -0.5 3,230,370 + HSERO_RS14210 0.15 -0.8
Or see this region's nucleotide sequence