Strain Fitness in Herbaspirillum seropedicae SmR1 around HSERO_RS10070

Experiment: Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntHSERO_RS10065 and HSERO_RS10070 are separated by 93 nucleotidesHSERO_RS10070 and HSERO_RS10075 are separated by 37 nucleotidesHSERO_RS10075 and HSERO_RS10080 are separated by 303 nucleotidesHSERO_RS10080 and HSERO_RS10085 are separated by 296 nucleotides HSERO_RS10065: HSERO_RS10065 - hypothetical protein, at 2,277,145 to 2,277,924 _RS10065 HSERO_RS10070: HSERO_RS10070 - hypothetical protein, at 2,278,018 to 2,278,419 _RS10070 HSERO_RS10075: HSERO_RS10075 - tRNA-Pro, at 2,278,457 to 2,278,533 _RS10075 HSERO_RS10080: HSERO_RS10080 - hypothetical protein, at 2,278,837 to 2,279,043 _RS10080 HSERO_RS10085: HSERO_RS10085 - transcriptional regulator, at 2,279,340 to 2,279,969 _RS10085 Position (kb) 2278 2279Strain fitness (log2 ratio) -1 0 1 2at 2277.730 kb on + strand, within HSERO_RS10065at 2277.779 kb on + strand, within HSERO_RS10065at 2278.024 kb on + strandat 2278.204 kb on - strand, within HSERO_RS10070at 2278.311 kb on + strand, within HSERO_RS10070at 2278.579 kb on + strandat 2278.633 kb on - strandat 2278.810 kb on + strandat 2278.924 kb on + strandat 2278.968 kb on - strand, within HSERO_RS10080at 2278.978 kb on - strand, within HSERO_RS10080at 2279.191 kb on - strandat 2279.226 kb on - strandat 2279.234 kb on + strandat 2279.236 kb on - strandat 2279.258 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB
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2,277,730 + HSERO_RS10065 0.75 +0.1
2,277,779 + HSERO_RS10065 0.81 +0.3
2,278,024 + +0.4
2,278,204 - HSERO_RS10070 0.46 -1.5
2,278,311 + HSERO_RS10070 0.73 +1.1
2,278,579 + +0.4
2,278,633 - -0.4
2,278,810 + +0.8
2,278,924 + +2.5
2,278,968 - HSERO_RS10080 0.63 -0.5
2,278,978 - HSERO_RS10080 0.68 -0.4
2,279,191 - +0.2
2,279,226 - +0.5
2,279,234 + +0.0
2,279,236 - -0.0
2,279,258 - +0.2

Or see this region's nucleotide sequence