Strain Fitness in Herbaspirillum seropedicae SmR1 around HSERO_RS10030

Experiment: Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntHSERO_RS10020 and HSERO_RS10025 are separated by 55 nucleotidesHSERO_RS10025 and HSERO_RS10030 are separated by 293 nucleotidesHSERO_RS10030 and HSERO_RS10035 overlap by 17 nucleotides HSERO_RS10020: HSERO_RS10020 - flippase, at 2,267,687 to 2,269,117 _RS10020 HSERO_RS10025: HSERO_RS10025 - hypothetical protein, at 2,269,173 to 2,269,787 _RS10025 HSERO_RS10030: HSERO_RS10030 - acyltransferase, at 2,270,081 to 2,271,019 _RS10030 HSERO_RS10035: HSERO_RS10035 - hypothetical protein, at 2,271,003 to 2,271,992 _RS10035 Position (kb) 2270 2271 2272Strain fitness (log2 ratio) -2 -1 0 1 2at 2270.113 kb on + strandat 2270.529 kb on + strand, within HSERO_RS10030at 2270.553 kb on + strand, within HSERO_RS10030at 2270.709 kb on - strand, within HSERO_RS10030at 2271.214 kb on - strand, within HSERO_RS10035at 2271.251 kb on - strand, within HSERO_RS10035at 2271.251 kb on - strand, within HSERO_RS10035at 2271.486 kb on - strand, within HSERO_RS10035at 2271.927 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB
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2,270,113 + -0.2
2,270,529 + HSERO_RS10030 0.48 +2.3
2,270,553 + HSERO_RS10030 0.50 +0.3
2,270,709 - HSERO_RS10030 0.67 -0.4
2,271,214 - HSERO_RS10035 0.21 +1.2
2,271,251 - HSERO_RS10035 0.25 +0.4
2,271,251 - HSERO_RS10035 0.25 -1.8
2,271,486 - HSERO_RS10035 0.49 -0.9
2,271,927 - +1.6

Or see this region's nucleotide sequence