Experiment: Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS08515 and HSERO_RS08520 are separated by 824 nucleotides HSERO_RS08520 and HSERO_RS08525 are separated by 59 nucleotides
HSERO_RS08515: HSERO_RS08515 - diguanylate cyclase, at 1,939,596 to 1,940,348
_RS08515
HSERO_RS08520: HSERO_RS08520 - major facilitator transporter, at 1,941,173 to 1,942,498
_RS08520
HSERO_RS08525: HSERO_RS08525 - hypothetical protein, at 1,942,558 to 1,943,256
_RS08525
Position (kb)
1941
1942
1943 Strain fitness (log2 ratio)
-2
-1
0
1 at 1940.385 kb on - strand at 1940.517 kb on + strand at 1940.606 kb on - strand at 1940.650 kb on - strand at 1940.817 kb on - strand at 1940.833 kb on - strand at 1940.833 kb on - strand at 1940.833 kb on - strand at 1940.833 kb on - strand at 1940.966 kb on + strand at 1940.966 kb on + strand at 1941.079 kb on - strand at 1941.250 kb on + strand at 1941.331 kb on + strand, within HSERO_RS08520 at 1941.552 kb on + strand, within HSERO_RS08520 at 1941.966 kb on - strand, within HSERO_RS08520 at 1941.994 kb on + strand, within HSERO_RS08520 at 1942.161 kb on - strand, within HSERO_RS08520 at 1942.280 kb on + strand, within HSERO_RS08520 at 1942.288 kb on - strand, within HSERO_RS08520 at 1942.288 kb on - strand, within HSERO_RS08520 at 1942.316 kb on - strand, within HSERO_RS08520 at 1942.640 kb on + strand, within HSERO_RS08525 at 1942.788 kb on + strand, within HSERO_RS08525 at 1942.788 kb on + strand, within HSERO_RS08525 at 1942.793 kb on + strand, within HSERO_RS08525 at 1942.855 kb on - strand, within HSERO_RS08525 at 1942.881 kb on + strand, within HSERO_RS08525 at 1943.118 kb on - strand, within HSERO_RS08525 at 1943.252 kb on + strand at 1943.265 kb on - strand at 1943.270 kb on - strand at 1943.307 kb on + strand at 1943.316 kb on - strand at 1943.379 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB remove 1,940,385 - +0.5 1,940,517 + -0.2 1,940,606 - +0.0 1,940,650 - +0.3 1,940,817 - -0.3 1,940,833 - -0.2 1,940,833 - -0.2 1,940,833 - -0.1 1,940,833 - -0.3 1,940,966 + -0.3 1,940,966 + +0.9 1,941,079 - -0.3 1,941,250 + +0.3 1,941,331 + HSERO_RS08520 0.12 +0.1 1,941,552 + HSERO_RS08520 0.29 -0.9 1,941,966 - HSERO_RS08520 0.60 +0.3 1,941,994 + HSERO_RS08520 0.62 +0.1 1,942,161 - HSERO_RS08520 0.75 +0.1 1,942,280 + HSERO_RS08520 0.83 +0.5 1,942,288 - HSERO_RS08520 0.84 +0.8 1,942,288 - HSERO_RS08520 0.84 -0.4 1,942,316 - HSERO_RS08520 0.86 -0.5 1,942,640 + HSERO_RS08525 0.12 +0.1 1,942,788 + HSERO_RS08525 0.33 -1.6 1,942,788 + HSERO_RS08525 0.33 -0.5 1,942,793 + HSERO_RS08525 0.34 -2.2 1,942,855 - HSERO_RS08525 0.42 -0.7 1,942,881 + HSERO_RS08525 0.46 -0.2 1,943,118 - HSERO_RS08525 0.80 -1.4 1,943,252 + -1.2 1,943,265 - -0.3 1,943,270 - -0.7 1,943,307 + -0.2 1,943,316 - -0.2 1,943,379 - +1.6
Or see this region's nucleotide sequence