Experiment: Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS05710 and HSERO_RS05715 are separated by 37 nucleotides HSERO_RS05715 and HSERO_RS05720 overlap by 14 nucleotides
HSERO_RS05710: HSERO_RS05710 - aldehyde dehydrogenase, at 1,296,866 to 1,298,326
_RS05710
HSERO_RS05715: HSERO_RS05715 - amidohydrolase, at 1,298,364 to 1,299,575
_RS05715
HSERO_RS05720: HSERO_RS05720 - alanine racemase, at 1,299,562 to 1,300,692
_RS05720
Position (kb)
1298
1299
1300 Strain fitness (log2 ratio)
-1
0
1
2 at 1297.451 kb on - strand, within HSERO_RS05710 at 1297.489 kb on - strand, within HSERO_RS05710 at 1297.537 kb on + strand, within HSERO_RS05710 at 1297.634 kb on - strand, within HSERO_RS05710 at 1297.861 kb on + strand, within HSERO_RS05710 at 1298.008 kb on - strand, within HSERO_RS05710 at 1298.048 kb on + strand, within HSERO_RS05710 at 1298.048 kb on + strand, within HSERO_RS05710 at 1298.147 kb on + strand, within HSERO_RS05710 at 1298.372 kb on + strand at 1298.380 kb on - strand at 1298.703 kb on - strand, within HSERO_RS05715 at 1298.775 kb on + strand, within HSERO_RS05715 at 1299.007 kb on + strand, within HSERO_RS05715 at 1299.124 kb on + strand, within HSERO_RS05715 at 1299.137 kb on - strand, within HSERO_RS05715 at 1299.333 kb on - strand, within HSERO_RS05715 at 1299.498 kb on + strand at 1299.503 kb on + strand at 1299.810 kb on + strand, within HSERO_RS05720 at 1299.858 kb on - strand, within HSERO_RS05720 at 1299.924 kb on + strand, within HSERO_RS05720 at 1299.925 kb on + strand, within HSERO_RS05720 at 1300.028 kb on + strand, within HSERO_RS05720 at 1300.061 kb on - strand, within HSERO_RS05720 at 1300.078 kb on - strand, within HSERO_RS05720 at 1300.180 kb on - strand, within HSERO_RS05720 at 1300.509 kb on - strand, within HSERO_RS05720
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB remove 1,297,451 - HSERO_RS05710 0.40 -0.2 1,297,489 - HSERO_RS05710 0.43 +0.7 1,297,537 + HSERO_RS05710 0.46 -0.5 1,297,634 - HSERO_RS05710 0.53 -0.0 1,297,861 + HSERO_RS05710 0.68 +0.4 1,298,008 - HSERO_RS05710 0.78 +1.3 1,298,048 + HSERO_RS05710 0.81 +2.0 1,298,048 + HSERO_RS05710 0.81 +0.5 1,298,147 + HSERO_RS05710 0.88 -0.2 1,298,372 + +0.3 1,298,380 - +0.6 1,298,703 - HSERO_RS05715 0.28 -0.5 1,298,775 + HSERO_RS05715 0.34 +0.4 1,299,007 + HSERO_RS05715 0.53 -0.4 1,299,124 + HSERO_RS05715 0.63 +0.1 1,299,137 - HSERO_RS05715 0.64 -0.1 1,299,333 - HSERO_RS05715 0.80 -1.4 1,299,498 + -0.0 1,299,503 + -0.2 1,299,810 + HSERO_RS05720 0.22 +0.1 1,299,858 - HSERO_RS05720 0.26 -0.8 1,299,924 + HSERO_RS05720 0.32 +0.1 1,299,925 + HSERO_RS05720 0.32 -1.0 1,300,028 + HSERO_RS05720 0.41 +1.5 1,300,061 - HSERO_RS05720 0.44 +0.2 1,300,078 - HSERO_RS05720 0.46 -0.4 1,300,180 - HSERO_RS05720 0.55 +0.3 1,300,509 - HSERO_RS05720 0.84 +0.7
Or see this region's nucleotide sequence