Experiment: Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS04385 and HSERO_RS04390 overlap by 11 nucleotides HSERO_RS04390 and HSERO_RS04395 are separated by 88 nucleotides HSERO_RS04395 and HSERO_RS04400 are separated by 79 nucleotides HSERO_RS04400 and HSERO_RS04405 overlap by 63 nucleotides HSERO_RS04405 and HSERO_RS04410 overlap by 10 nucleotides HSERO_RS04410 and HSERO_RS04415 overlap by 8 nucleotides HSERO_RS04415 and HSERO_RS04420 are separated by 313 nucleotides
HSERO_RS04385: HSERO_RS04385 - replication protein, at 971,843 to 973,894
_RS04385
HSERO_RS04390: HSERO_RS04390 - hypothetical protein, at 973,884 to 974,237
_RS04390
HSERO_RS04395: HSERO_RS04395 - hypothetical protein, at 974,326 to 974,532
_RS04395
HSERO_RS04400: HSERO_RS04400 - transcriptional regulator, at 974,612 to 974,806
_RS04400
HSERO_RS04405: HSERO_RS04405 - transcriptional regulator, at 974,744 to 974,872
_RS04405
HSERO_RS04410: HSERO_RS04410 - hypothetical protein, at 974,863 to 975,060
_RS04410
HSERO_RS04415: HSERO_RS04415 - hypothetical protein, at 975,053 to 975,295
_RS04415
HSERO_RS04420: HSERO_RS04420 - DNA-binding transcriptional regulator, at 975,609 to 976,046
_RS04420
Position (kb)
974
975 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 973.849 kb on - strand at 973.856 kb on - strand at 973.938 kb on + strand, within HSERO_RS04390 at 973.946 kb on - strand, within HSERO_RS04390 at 973.946 kb on - strand, within HSERO_RS04390 at 974.118 kb on + strand, within HSERO_RS04390 at 974.341 kb on - strand at 974.527 kb on + strand at 974.551 kb on + strand at 974.627 kb on + strand at 974.635 kb on - strand, within HSERO_RS04400 at 974.708 kb on + strand, within HSERO_RS04400 at 974.708 kb on + strand, within HSERO_RS04400 at 974.708 kb on + strand, within HSERO_RS04400 at 974.716 kb on - strand, within HSERO_RS04400 at 974.736 kb on - strand, within HSERO_RS04400 at 974.747 kb on + strand at 974.755 kb on - strand at 974.813 kb on + strand, within HSERO_RS04405 at 974.821 kb on - strand, within HSERO_RS04405 at 974.861 kb on + strand at 974.908 kb on - strand, within HSERO_RS04410 at 974.940 kb on - strand, within HSERO_RS04410 at 975.014 kb on + strand, within HSERO_RS04410 at 975.014 kb on + strand at 975.022 kb on - strand, within HSERO_RS04410 at 975.022 kb on - strand, within HSERO_RS04410 at 975.035 kb on + strand, within HSERO_RS04410 at 975.035 kb on + strand, within HSERO_RS04410 at 975.045 kb on + strand at 975.125 kb on - strand, within HSERO_RS04415 at 975.146 kb on - strand, within HSERO_RS04415 at 975.153 kb on + strand at 975.153 kb on + strand, within HSERO_RS04415 at 975.153 kb on + strand, within HSERO_RS04415 at 975.161 kb on - strand, within HSERO_RS04415 at 975.161 kb on - strand, within HSERO_RS04415 at 975.161 kb on - strand, within HSERO_RS04415 at 975.161 kb on - strand, within HSERO_RS04415 at 975.163 kb on + strand, within HSERO_RS04415 at 975.163 kb on + strand, within HSERO_RS04415 at 975.171 kb on - strand, within HSERO_RS04415 at 975.171 kb on - strand, within HSERO_RS04415 at 975.171 kb on - strand at 975.171 kb on - strand, within HSERO_RS04415 at 975.173 kb on - strand, within HSERO_RS04415 at 975.173 kb on - strand, within HSERO_RS04415 at 975.173 kb on - strand, within HSERO_RS04415 at 975.183 kb on + strand, within HSERO_RS04415 at 975.301 kb on + strand at 975.310 kb on - strand at 975.310 kb on - strand at 975.335 kb on - strand at 975.352 kb on - strand at 975.396 kb on + strand at 975.396 kb on + strand at 975.404 kb on - strand at 975.404 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB remove 973,849 - -0.3 973,856 - +0.5 973,938 + HSERO_RS04390 0.15 +0.2 973,946 - HSERO_RS04390 0.18 -0.8 973,946 - HSERO_RS04390 0.18 +0.1 974,118 + HSERO_RS04390 0.66 -0.0 974,341 - -0.6 974,527 + -0.4 974,551 + +0.2 974,627 + -0.0 974,635 - HSERO_RS04400 0.12 -0.6 974,708 + HSERO_RS04400 0.49 -0.1 974,708 + HSERO_RS04400 0.49 -0.2 974,708 + HSERO_RS04400 0.49 -0.2 974,716 - HSERO_RS04400 0.53 -0.3 974,736 - HSERO_RS04400 0.64 -0.6 974,747 + +0.7 974,755 - -0.9 974,813 + HSERO_RS04405 0.53 +1.1 974,821 - HSERO_RS04405 0.60 +0.2 974,861 + -0.5 974,908 - HSERO_RS04410 0.23 -0.3 974,940 - HSERO_RS04410 0.39 +0.2 975,014 + HSERO_RS04410 0.76 +0.8 975,014 + -1.4 975,022 - HSERO_RS04410 0.80 +0.4 975,022 - HSERO_RS04410 0.80 +0.6 975,035 + HSERO_RS04410 0.87 +0.0 975,035 + HSERO_RS04410 0.87 -0.5 975,045 + +2.5 975,125 - HSERO_RS04415 0.30 +0.1 975,146 - HSERO_RS04415 0.38 +0.1 975,153 + +0.9 975,153 + HSERO_RS04415 0.41 -0.2 975,153 + HSERO_RS04415 0.41 +0.3 975,161 - HSERO_RS04415 0.44 -0.7 975,161 - HSERO_RS04415 0.44 +0.1 975,161 - HSERO_RS04415 0.44 -1.1 975,161 - HSERO_RS04415 0.44 +0.4 975,163 + HSERO_RS04415 0.45 +0.0 975,163 + HSERO_RS04415 0.45 -0.2 975,171 - HSERO_RS04415 0.49 -0.2 975,171 - HSERO_RS04415 0.49 -0.9 975,171 - +0.9 975,171 - HSERO_RS04415 0.49 -0.3 975,173 - HSERO_RS04415 0.49 -1.8 975,173 - HSERO_RS04415 0.49 -0.1 975,173 - HSERO_RS04415 0.49 +0.4 975,183 + HSERO_RS04415 0.53 +0.1 975,301 + +0.0 975,310 - +0.6 975,310 - +0.3 975,335 - -0.2 975,352 - +2.8 975,396 + -0.4 975,396 + -0.7 975,404 - -0.4 975,404 - +0.8
Or see this region's nucleotide sequence