Experiment: SBM - with RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ddlA and asnS are separated by 244 nucleotides asnS and SO2219 are separated by 195 nucleotides SO2219 and SO2220 are separated by 17 nucleotides
SO2217: ddlA - D-alanine--D-alanine ligase (NCBI ptt file), at 2,331,687 to 2,332,697
ddlA
SO2218: asnS - asparaginyl-tRNA synthetase (NCBI ptt file), at 2,332,942 to 2,334,342
asnS
SO2219: SO2219 - hypothetical protein (NCBI ptt file), at 2,334,538 to 2,334,879
SO2219
SO2220: SO2220 - MutT/nudix family protein (NCBI ptt file), at 2,334,897 to 2,335,484
SO2220
Position (kb)
2332
2333
2334
2335 Strain fitness (log2 ratio)
-2
-1
0
1 at 2332.883 kb on - strand at 2332.883 kb on - strand at 2334.453 kb on - strand at 2334.472 kb on - strand at 2334.493 kb on + strand at 2334.638 kb on - strand, within SO2219 at 2334.644 kb on - strand, within SO2219 at 2334.685 kb on - strand, within SO2219 at 2334.789 kb on - strand, within SO2219 at 2334.913 kb on + strand at 2334.918 kb on + strand at 2335.000 kb on + strand, within SO2220 at 2335.013 kb on - strand, within SO2220 at 2335.257 kb on - strand, within SO2220
Per-strain Table
Position Strand Gene LocusTag Fraction SBM - with RNA protect remove 2,332,883 - -0.3 2,332,883 - +0.2 2,334,453 - +0.6 2,334,472 - +0.3 2,334,493 + +0.3 2,334,638 - SO2219 0.29 +1.0 2,334,644 - SO2219 0.31 +0.1 2,334,685 - SO2219 0.43 +1.0 2,334,789 - SO2219 0.73 +1.0 2,334,913 + -0.7 2,334,918 + +1.6 2,335,000 + SO2220 0.18 +0.9 2,335,013 - SO2220 0.20 +0.5 2,335,257 - SO2220 0.61 -1.9
Or see this region's nucleotide sequence