Strain Fitness in Shewanella oneidensis MR-1 around SO2093

Experiment: SBM - with RNA protect

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthypD and hypC are separated by 62 nucleotideshypC and hypB overlap by 1 nucleotideshypB and hypF are separated by 226 nucleotides SO2091: hypD - hydrogenase expression/formation protein HypD (NCBI ptt file), at 2,187,032 to 2,188,171 hypD SO2092: hypC - hydrogenase assembly chaperone hypC/hupF (NCBI ptt file), at 2,188,234 to 2,188,479 hypC SO2093: hypB - hydrogenase accessory protein HypB (NCBI ptt file), at 2,188,479 to 2,189,273 hypB SO2094: hypF - hydrogenase maturation protein HypF (NCBI ptt file), at 2,189,500 to 2,191,926 hypF Position (kb) 2188 2189 2190Strain fitness (log2 ratio) -2 -1 0 1 2at 2187.537 kb on - strand, within hypDat 2187.557 kb on + strand, within hypDat 2187.566 kb on + strand, within hypDat 2187.595 kb on - strand, within hypDat 2187.718 kb on + strand, within hypDat 2187.780 kb on + strand, within hypDat 2187.880 kb on + strand, within hypDat 2187.880 kb on + strand, within hypDat 2187.903 kb on + strand, within hypDat 2187.949 kb on + strand, within hypDat 2188.070 kb on - strandat 2188.075 kb on + strandat 2188.289 kb on - strand, within hypCat 2188.404 kb on + strand, within hypCat 2188.404 kb on + strand, within hypCat 2188.404 kb on + strand, within hypCat 2188.509 kb on - strandat 2188.853 kb on + strand, within hypBat 2188.861 kb on - strand, within hypBat 2188.861 kb on - strand, within hypBat 2188.954 kb on + strand, within hypBat 2188.975 kb on + strand, within hypBat 2188.996 kb on + strand, within hypBat 2189.004 kb on - strand, within hypBat 2189.060 kb on - strand, within hypBat 2189.079 kb on + strand, within hypBat 2189.079 kb on + strand, within hypBat 2189.158 kb on - strand, within hypBat 2189.472 kb on - strandat 2189.513 kb on + strandat 2189.545 kb on - strandat 2189.685 kb on + strandat 2189.721 kb on + strandat 2189.721 kb on + strandat 2189.729 kb on - strandat 2189.781 kb on + strand, within hypFat 2190.027 kb on - strand, within hypFat 2190.222 kb on + strand, within hypFat 2190.267 kb on - strand, within hypF

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Per-strain Table

Position Strand Gene LocusTag Fraction SBM - with RNA protect
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2,187,537 - hypD SO2091 0.44 +0.2
2,187,557 + hypD SO2091 0.46 +0.8
2,187,566 + hypD SO2091 0.47 +0.1
2,187,595 - hypD SO2091 0.49 +0.9
2,187,718 + hypD SO2091 0.60 -0.1
2,187,780 + hypD SO2091 0.66 -0.3
2,187,880 + hypD SO2091 0.74 +1.1
2,187,880 + hypD SO2091 0.74 +0.9
2,187,903 + hypD SO2091 0.76 -1.8
2,187,949 + hypD SO2091 0.80 -1.7
2,188,070 - -0.6
2,188,075 + -0.3
2,188,289 - hypC SO2092 0.22 -0.5
2,188,404 + hypC SO2092 0.69 -0.3
2,188,404 + hypC SO2092 0.69 -2.6
2,188,404 + hypC SO2092 0.69 -0.7
2,188,509 - +1.4
2,188,853 + hypB SO2093 0.47 +0.5
2,188,861 - hypB SO2093 0.48 -2.0
2,188,861 - hypB SO2093 0.48 -0.3
2,188,954 + hypB SO2093 0.60 +0.1
2,188,975 + hypB SO2093 0.62 -0.4
2,188,996 + hypB SO2093 0.65 -0.2
2,189,004 - hypB SO2093 0.66 +0.6
2,189,060 - hypB SO2093 0.73 -2.2
2,189,079 + hypB SO2093 0.75 -2.5
2,189,079 + hypB SO2093 0.75 -0.5
2,189,158 - hypB SO2093 0.85 -0.4
2,189,472 - +0.1
2,189,513 + +0.6
2,189,545 - -0.9
2,189,685 + -1.0
2,189,721 + -0.5
2,189,721 + -0.1
2,189,729 - +0.1
2,189,781 + hypF SO2094 0.12 +0.3
2,190,027 - hypF SO2094 0.22 +2.2
2,190,222 + hypF SO2094 0.30 -0.1
2,190,267 - hypF SO2094 0.32 +0.5

Or see this region's nucleotide sequence