Experiment: SBM - with RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO_1620 and SO1622 are separated by 386 nucleotides SO1622 and ptsG overlap by 49 nucleotides
SO_1620: SO_1620 - no description, at 1,702,477 to 1,703,313
_1620
SO1622: SO1622 - hypothetical flavodoxin (NCBI ptt file), at 1,703,700 to 1,704,164
SO1622
SO1623: ptsG - PTS system, glucose-specific IIBC component (NCBI ptt file), at 1,704,116 to 1,705,615
ptsG
Position (kb)
1703
1704
1705 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1702.827 kb on - strand, within SO_1620 at 1702.889 kb on - strand, within SO_1620 at 1702.889 kb on - strand, within SO_1620 at 1702.911 kb on + strand, within SO_1620 at 1703.180 kb on + strand, within SO_1620 at 1703.187 kb on + strand, within SO_1620 at 1703.195 kb on - strand, within SO_1620 at 1703.308 kb on + strand at 1703.316 kb on - strand at 1703.316 kb on - strand at 1703.320 kb on + strand at 1703.327 kb on + strand at 1703.327 kb on + strand at 1703.397 kb on + strand at 1703.471 kb on - strand at 1703.587 kb on - strand at 1703.634 kb on + strand at 1703.730 kb on + strand at 1703.730 kb on + strand at 1703.730 kb on + strand at 1703.730 kb on + strand at 1703.730 kb on + strand at 1703.730 kb on + strand at 1703.738 kb on - strand at 1703.738 kb on - strand at 1703.738 kb on - strand at 1703.738 kb on - strand at 1703.738 kb on - strand at 1703.738 kb on - strand at 1703.738 kb on - strand at 1703.738 kb on - strand at 1703.738 kb on - strand at 1703.754 kb on + strand, within SO1622 at 1703.877 kb on + strand, within SO1622 at 1703.939 kb on + strand, within SO1622 at 1703.954 kb on + strand, within SO1622 at 1704.050 kb on + strand, within SO1622 at 1704.148 kb on - strand at 1704.400 kb on - strand, within ptsG at 1704.538 kb on + strand, within ptsG at 1704.573 kb on - strand, within ptsG at 1704.656 kb on + strand, within ptsG at 1704.689 kb on + strand, within ptsG at 1704.689 kb on + strand, within ptsG at 1704.721 kb on - strand, within ptsG at 1704.794 kb on + strand, within ptsG at 1704.845 kb on + strand, within ptsG at 1704.865 kb on + strand, within ptsG at 1705.013 kb on + strand, within ptsG at 1705.113 kb on + strand, within ptsG
Per-strain Table
Position Strand Gene LocusTag Fraction SBM - with RNA protect remove 1,702,827 - SO_1620 0.42 -0.6 1,702,889 - SO_1620 0.49 -0.2 1,702,889 - SO_1620 0.49 -1.0 1,702,911 + SO_1620 0.52 -3.2 1,703,180 + SO_1620 0.84 -1.8 1,703,187 + SO_1620 0.85 +0.2 1,703,195 - SO_1620 0.86 +0.6 1,703,308 + -0.1 1,703,316 - -0.3 1,703,316 - -0.1 1,703,320 + +0.7 1,703,327 + -0.5 1,703,327 + +0.2 1,703,397 + +0.6 1,703,471 - +0.3 1,703,587 - -0.3 1,703,634 + +0.1 1,703,730 + +2.0 1,703,730 + +0.0 1,703,730 + -0.7 1,703,730 + +1.1 1,703,730 + +1.1 1,703,730 + +0.0 1,703,738 - -0.3 1,703,738 - -0.2 1,703,738 - -0.3 1,703,738 - -0.4 1,703,738 - +0.4 1,703,738 - +0.3 1,703,738 - -0.0 1,703,738 - -0.1 1,703,738 - +1.2 1,703,754 + SO1622 0.12 -0.3 1,703,877 + SO1622 0.38 -0.7 1,703,939 + SO1622 0.51 -0.6 1,703,954 + SO1622 0.55 +0.1 1,704,050 + SO1622 0.75 +0.3 1,704,148 - +0.3 1,704,400 - ptsG SO1623 0.19 -0.0 1,704,538 + ptsG SO1623 0.28 +0.2 1,704,573 - ptsG SO1623 0.30 -0.3 1,704,656 + ptsG SO1623 0.36 -1.3 1,704,689 + ptsG SO1623 0.38 -0.1 1,704,689 + ptsG SO1623 0.38 +0.3 1,704,721 - ptsG SO1623 0.40 -1.3 1,704,794 + ptsG SO1623 0.45 -2.2 1,704,845 + ptsG SO1623 0.49 -0.4 1,704,865 + ptsG SO1623 0.50 -0.1 1,705,013 + ptsG SO1623 0.60 -0.3 1,705,113 + ptsG SO1623 0.66 +1.0
Or see this region's nucleotide sequence