Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26750 and BWI76_RS26755 are separated by 183 nucleotides BWI76_RS26755 and BWI76_RS26760 are separated by 221 nucleotides
BWI76_RS26750: BWI76_RS26750 - PfkB domain-containing protein, at 5,539,645 to 5,540,580
_RS26750
BWI76_RS26755: BWI76_RS26755 - peptidase M16, at 5,540,764 to 5,542,269
_RS26755
BWI76_RS26760: BWI76_RS26760 - C4-dicarboxylate transport protein DctA (from data) , at 5,542,491 to 5,543,777
_RS26760
Position (kb)
5540
5541
5542
5543 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5539.900 kb on + strand, within BWI76_RS26750 at 5539.900 kb on + strand, within BWI76_RS26750 at 5540.003 kb on - strand, within BWI76_RS26750 at 5540.009 kb on + strand, within BWI76_RS26750 at 5540.024 kb on - strand, within BWI76_RS26750 at 5540.295 kb on - strand, within BWI76_RS26750 at 5540.295 kb on - strand, within BWI76_RS26750 at 5540.635 kb on + strand at 5540.697 kb on + strand at 5540.941 kb on - strand, within BWI76_RS26755 at 5541.095 kb on - strand, within BWI76_RS26755 at 5541.284 kb on - strand, within BWI76_RS26755 at 5541.292 kb on + strand, within BWI76_RS26755 at 5541.426 kb on + strand, within BWI76_RS26755 at 5541.434 kb on - strand, within BWI76_RS26755 at 5541.484 kb on - strand, within BWI76_RS26755 at 5541.620 kb on - strand, within BWI76_RS26755 at 5541.951 kb on + strand, within BWI76_RS26755 at 5541.961 kb on - strand, within BWI76_RS26755 at 5541.987 kb on - strand, within BWI76_RS26755 at 5541.987 kb on - strand, within BWI76_RS26755 at 5541.987 kb on - strand, within BWI76_RS26755 at 5542.097 kb on - strand, within BWI76_RS26755 at 5542.134 kb on - strand at 5542.134 kb on - strand at 5542.211 kb on - strand at 5542.270 kb on - strand at 5542.359 kb on - strand at 5542.413 kb on - strand at 5542.531 kb on - strand at 5542.561 kb on - strand at 5542.568 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 5,539,900 + BWI76_RS26750 0.27 -1.6 5,539,900 + BWI76_RS26750 0.27 -1.0 5,540,003 - BWI76_RS26750 0.38 +0.1 5,540,009 + BWI76_RS26750 0.39 +0.7 5,540,024 - BWI76_RS26750 0.40 -2.8 5,540,295 - BWI76_RS26750 0.69 +0.9 5,540,295 - BWI76_RS26750 0.69 -0.3 5,540,635 + -0.3 5,540,697 + -0.2 5,540,941 - BWI76_RS26755 0.12 -0.1 5,541,095 - BWI76_RS26755 0.22 -0.3 5,541,284 - BWI76_RS26755 0.35 +0.3 5,541,292 + BWI76_RS26755 0.35 +0.6 5,541,426 + BWI76_RS26755 0.44 -0.1 5,541,434 - BWI76_RS26755 0.44 +0.8 5,541,484 - BWI76_RS26755 0.48 +1.2 5,541,620 - BWI76_RS26755 0.57 +0.3 5,541,951 + BWI76_RS26755 0.79 -1.0 5,541,961 - BWI76_RS26755 0.79 -0.7 5,541,987 - BWI76_RS26755 0.81 -0.0 5,541,987 - BWI76_RS26755 0.81 +1.6 5,541,987 - BWI76_RS26755 0.81 +0.1 5,542,097 - BWI76_RS26755 0.89 +0.3 5,542,134 - +0.5 5,542,134 - +0.4 5,542,211 - +0.2 5,542,270 - -1.9 5,542,359 - -1.0 5,542,413 - -0.5 5,542,531 - -1.1 5,542,561 - -0.7 5,542,568 + -0.0
Or see this region's nucleotide sequence