Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25955

Experiment: RCH2_defined_glucose with MS_media 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25950 and BWI76_RS25955 are separated by 4 nucleotidesBWI76_RS25955 and BWI76_RS25960 are separated by 196 nucleotidesBWI76_RS25960 and BWI76_RS25965 are separated by 81 nucleotides BWI76_RS25950: BWI76_RS25950 - nicotinate phosphoribosyltransferase, at 5,364,835 to 5,366,325 _RS25950 BWI76_RS25955: BWI76_RS25955 - ribose-phosphate pyrophosphokinase, at 5,366,330 to 5,367,199 _RS25955 BWI76_RS25960: BWI76_RS25960 - NUDIX hydrolase, at 5,367,396 to 5,368,115 _RS25960 BWI76_RS25965: BWI76_RS25965 - hydrolase, at 5,368,197 to 5,369,219 _RS25965 Position (kb) 5366 5367 5368Strain fitness (log2 ratio) -1 0 1at 5365.408 kb on + strand, within BWI76_RS25950at 5365.416 kb on - strand, within BWI76_RS25950at 5365.686 kb on + strand, within BWI76_RS25950at 5365.724 kb on + strand, within BWI76_RS25950at 5365.724 kb on + strand, within BWI76_RS25950at 5365.727 kb on - strand, within BWI76_RS25950at 5365.727 kb on - strand, within BWI76_RS25950at 5365.732 kb on - strand, within BWI76_RS25950at 5365.870 kb on - strand, within BWI76_RS25950at 5365.934 kb on - strand, within BWI76_RS25950at 5366.113 kb on + strand, within BWI76_RS25950at 5366.136 kb on - strand, within BWI76_RS25950at 5366.453 kb on + strandat 5366.499 kb on - strand, within BWI76_RS25955at 5366.516 kb on + strand, within BWI76_RS25955at 5366.521 kb on + strand, within BWI76_RS25955at 5366.522 kb on + strand, within BWI76_RS25955at 5366.564 kb on - strand, within BWI76_RS25955at 5366.619 kb on - strand, within BWI76_RS25955at 5366.624 kb on - strand, within BWI76_RS25955at 5366.705 kb on - strand, within BWI76_RS25955at 5366.760 kb on + strand, within BWI76_RS25955at 5366.782 kb on + strand, within BWI76_RS25955at 5366.782 kb on + strand, within BWI76_RS25955at 5366.788 kb on - strand, within BWI76_RS25955at 5366.790 kb on - strand, within BWI76_RS25955at 5366.876 kb on + strand, within BWI76_RS25955at 5367.144 kb on + strandat 5367.392 kb on + strandat 5367.392 kb on + strandat 5367.485 kb on + strandat 5367.493 kb on - strand, within BWI76_RS25960at 5367.603 kb on + strand, within BWI76_RS25960at 5367.739 kb on + strand, within BWI76_RS25960at 5367.800 kb on - strand, within BWI76_RS25960at 5367.877 kb on + strand, within BWI76_RS25960at 5367.900 kb on + strand, within BWI76_RS25960at 5367.914 kb on - strand, within BWI76_RS25960at 5367.963 kb on - strand, within BWI76_RS25960at 5368.096 kb on - strandat 5368.122 kb on + strandat 5368.130 kb on - strandat 5368.197 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x
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5,365,408 + BWI76_RS25950 0.38 +0.1
5,365,416 - BWI76_RS25950 0.39 -0.5
5,365,686 + BWI76_RS25950 0.57 -0.5
5,365,724 + BWI76_RS25950 0.60 +0.4
5,365,724 + BWI76_RS25950 0.60 +0.5
5,365,727 - BWI76_RS25950 0.60 +0.2
5,365,727 - BWI76_RS25950 0.60 +1.4
5,365,732 - BWI76_RS25950 0.60 +0.3
5,365,870 - BWI76_RS25950 0.69 +0.6
5,365,934 - BWI76_RS25950 0.74 -0.0
5,366,113 + BWI76_RS25950 0.86 +0.7
5,366,136 - BWI76_RS25950 0.87 -0.6
5,366,453 + +0.1
5,366,499 - BWI76_RS25955 0.19 +0.6
5,366,516 + BWI76_RS25955 0.21 +0.2
5,366,521 + BWI76_RS25955 0.22 -0.5
5,366,522 + BWI76_RS25955 0.22 +0.3
5,366,564 - BWI76_RS25955 0.27 -0.3
5,366,619 - BWI76_RS25955 0.33 +0.1
5,366,624 - BWI76_RS25955 0.34 +0.5
5,366,705 - BWI76_RS25955 0.43 +1.1
5,366,760 + BWI76_RS25955 0.49 +0.0
5,366,782 + BWI76_RS25955 0.52 -0.4
5,366,782 + BWI76_RS25955 0.52 +0.5
5,366,788 - BWI76_RS25955 0.53 +0.1
5,366,790 - BWI76_RS25955 0.53 +1.3
5,366,876 + BWI76_RS25955 0.63 -0.3
5,367,144 + -0.3
5,367,392 + +0.1
5,367,392 + +0.3
5,367,485 + +0.8
5,367,493 - BWI76_RS25960 0.13 +0.4
5,367,603 + BWI76_RS25960 0.29 +0.8
5,367,739 + BWI76_RS25960 0.48 +0.6
5,367,800 - BWI76_RS25960 0.56 -1.2
5,367,877 + BWI76_RS25960 0.67 -1.1
5,367,900 + BWI76_RS25960 0.70 +0.1
5,367,914 - BWI76_RS25960 0.72 +0.5
5,367,963 - BWI76_RS25960 0.79 +0.9
5,368,096 - +0.1
5,368,122 + +0.1
5,368,130 - +0.1
5,368,197 + -1.2

Or see this region's nucleotide sequence