Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS22640

Experiment: RCH2_defined_glucose with MS_media 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS22635 and BWI76_RS22640 overlap by 4 nucleotidesBWI76_RS22640 and BWI76_RS22645 are separated by 65 nucleotides BWI76_RS22635: BWI76_RS22635 - MFS transporter, at 4,718,449 to 4,719,663 _RS22635 BWI76_RS22640: BWI76_RS22640 - hypothetical protein, at 4,719,660 to 4,720,037 _RS22640 BWI76_RS22645: BWI76_RS22645 - RNA polymerase sigma factor RpoS, at 4,720,103 to 4,721,095 _RS22645 Position (kb) 4719 4720 4721Strain fitness (log2 ratio) -1 0 1at 4718.763 kb on - strand, within BWI76_RS22635at 4719.000 kb on + strand, within BWI76_RS22635at 4719.391 kb on + strand, within BWI76_RS22635at 4719.399 kb on - strand, within BWI76_RS22635at 4719.458 kb on - strand, within BWI76_RS22635at 4719.536 kb on + strand, within BWI76_RS22635at 4719.652 kb on - strandat 4719.904 kb on + strand, within BWI76_RS22640at 4719.917 kb on - strand, within BWI76_RS22640at 4720.041 kb on - strandat 4720.112 kb on + strandat 4720.220 kb on + strand, within BWI76_RS22645at 4720.220 kb on + strand, within BWI76_RS22645at 4720.258 kb on + strand, within BWI76_RS22645at 4720.279 kb on - strand, within BWI76_RS22645at 4720.785 kb on - strand, within BWI76_RS22645at 4720.919 kb on - strand, within BWI76_RS22645at 4721.005 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x
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4,718,763 - BWI76_RS22635 0.26 -0.6
4,719,000 + BWI76_RS22635 0.45 -0.1
4,719,391 + BWI76_RS22635 0.78 +0.0
4,719,399 - BWI76_RS22635 0.78 -0.6
4,719,458 - BWI76_RS22635 0.83 -0.1
4,719,536 + BWI76_RS22635 0.89 -0.9
4,719,652 - -0.3
4,719,904 + BWI76_RS22640 0.65 -1.2
4,719,917 - BWI76_RS22640 0.68 -0.5
4,720,041 - +0.6
4,720,112 + +0.5
4,720,220 + BWI76_RS22645 0.12 +0.2
4,720,220 + BWI76_RS22645 0.12 -0.1
4,720,258 + BWI76_RS22645 0.16 -0.2
4,720,279 - BWI76_RS22645 0.18 -0.0
4,720,785 - BWI76_RS22645 0.69 +0.5
4,720,919 - BWI76_RS22645 0.82 -0.3
4,721,005 - +0.0

Or see this region's nucleotide sequence