Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20940

Experiment: RCH2_defined_glucose with MS_media 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20930 and BWI76_RS20935 are separated by 28 nucleotidesBWI76_RS20935 and BWI76_RS20940 are separated by 71 nucleotidesBWI76_RS20940 and BWI76_RS20945 are separated by 95 nucleotidesBWI76_RS20945 and BWI76_RS20950 overlap by 63 nucleotidesBWI76_RS20950 and BWI76_RS20955 are separated by 54 nucleotides BWI76_RS20930: BWI76_RS20930 - arsenate reductase (glutaredoxin), at 4,376,392 to 4,376,751 _RS20930 BWI76_RS20935: BWI76_RS20935 - DnaA regulatory inactivator Hda, at 4,376,780 to 4,377,505 _RS20935 BWI76_RS20940: BWI76_RS20940 - uracil/xanthine transporter, at 4,377,577 to 4,378,866 _RS20940 BWI76_RS20945: BWI76_RS20945 - uracil phosphoribosyltransferase, at 4,378,962 to 4,379,588 _RS20945 BWI76_RS20950: BWI76_RS20950 - hypothetical protein, at 4,379,526 to 4,379,716 _RS20950 BWI76_RS20955: BWI76_RS20955 - 6-phospho-beta-glucosidase, at 4,379,771 to 4,381,201 _RS20955 Position (kb) 4377 4378 4379Strain fitness (log2 ratio) -2 -1 0 1at 4376.761 kb on + strandat 4377.517 kb on + strandat 4377.548 kb on - strandat 4377.568 kb on + strandat 4377.576 kb on - strandat 4377.703 kb on - strandat 4377.768 kb on - strand, within BWI76_RS20940at 4378.272 kb on + strand, within BWI76_RS20940at 4378.730 kb on + strand, within BWI76_RS20940at 4378.758 kb on - strandat 4379.050 kb on + strand, within BWI76_RS20945at 4379.060 kb on - strand, within BWI76_RS20945at 4379.261 kb on + strand, within BWI76_RS20945at 4379.477 kb on - strand, within BWI76_RS20945at 4379.701 kb on + strandat 4379.701 kb on + strandat 4379.709 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x
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4,376,761 + +0.2
4,377,517 + -1.1
4,377,548 - -0.3
4,377,568 + -0.4
4,377,576 - +0.2
4,377,703 - -0.7
4,377,768 - BWI76_RS20940 0.15 -0.4
4,378,272 + BWI76_RS20940 0.54 -2.2
4,378,730 + BWI76_RS20940 0.89 -0.7
4,378,758 - -2.0
4,379,050 + BWI76_RS20945 0.14 -0.5
4,379,060 - BWI76_RS20945 0.16 -0.3
4,379,261 + BWI76_RS20945 0.48 +0.2
4,379,477 - BWI76_RS20945 0.82 +0.9
4,379,701 + -0.7
4,379,701 + -0.3
4,379,709 - -0.2

Or see this region's nucleotide sequence