Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20930 and BWI76_RS20935 are separated by 28 nucleotides BWI76_RS20935 and BWI76_RS20940 are separated by 71 nucleotides BWI76_RS20940 and BWI76_RS20945 are separated by 95 nucleotides BWI76_RS20945 and BWI76_RS20950 overlap by 63 nucleotides BWI76_RS20950 and BWI76_RS20955 are separated by 54 nucleotides
BWI76_RS20930: BWI76_RS20930 - arsenate reductase (glutaredoxin), at 4,376,392 to 4,376,751
_RS20930
BWI76_RS20935: BWI76_RS20935 - DnaA regulatory inactivator Hda, at 4,376,780 to 4,377,505
_RS20935
BWI76_RS20940: BWI76_RS20940 - uracil/xanthine transporter, at 4,377,577 to 4,378,866
_RS20940
BWI76_RS20945: BWI76_RS20945 - uracil phosphoribosyltransferase, at 4,378,962 to 4,379,588
_RS20945
BWI76_RS20950: BWI76_RS20950 - hypothetical protein, at 4,379,526 to 4,379,716
_RS20950
BWI76_RS20955: BWI76_RS20955 - 6-phospho-beta-glucosidase, at 4,379,771 to 4,381,201
_RS20955
Position (kb)
4377
4378
4379 Strain fitness (log2 ratio)
-2
-1
0
1 at 4376.761 kb on + strand at 4377.517 kb on + strand at 4377.548 kb on - strand at 4377.568 kb on + strand at 4377.576 kb on - strand at 4377.703 kb on - strand at 4377.768 kb on - strand, within BWI76_RS20940 at 4378.272 kb on + strand, within BWI76_RS20940 at 4378.730 kb on + strand, within BWI76_RS20940 at 4378.758 kb on - strand at 4379.050 kb on + strand, within BWI76_RS20945 at 4379.060 kb on - strand, within BWI76_RS20945 at 4379.261 kb on + strand, within BWI76_RS20945 at 4379.477 kb on - strand, within BWI76_RS20945 at 4379.701 kb on + strand at 4379.701 kb on + strand at 4379.709 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 4,376,761 + +0.2 4,377,517 + -1.1 4,377,548 - -0.3 4,377,568 + -0.4 4,377,576 - +0.2 4,377,703 - -0.7 4,377,768 - BWI76_RS20940 0.15 -0.4 4,378,272 + BWI76_RS20940 0.54 -2.2 4,378,730 + BWI76_RS20940 0.89 -0.7 4,378,758 - -2.0 4,379,050 + BWI76_RS20945 0.14 -0.5 4,379,060 - BWI76_RS20945 0.16 -0.3 4,379,261 + BWI76_RS20945 0.48 +0.2 4,379,477 - BWI76_RS20945 0.82 +0.9 4,379,701 + -0.7 4,379,701 + -0.3 4,379,709 - -0.2
Or see this region's nucleotide sequence