Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14540

Experiment: RCH2_defined_glucose with MS_media 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14535 and BWI76_RS14540 overlap by 4 nucleotidesBWI76_RS14540 and BWI76_RS14545 overlap by 4 nucleotidesBWI76_RS14545 and BWI76_RS14550 are separated by 3 nucleotides BWI76_RS14535: BWI76_RS14535 - polyketide synthase, at 3,042,203 to 3,051,679 _RS14535 BWI76_RS14540: BWI76_RS14540 - yersiniabactin biosynthetic protein YbtU, at 3,051,676 to 3,052,773 _RS14540 BWI76_RS14545: BWI76_RS14545 - thioesterase, at 3,052,770 to 3,053,573 _RS14545 BWI76_RS14550: BWI76_RS14550 - 2,3-dihydroxybenzoate-AMP ligase, at 3,053,577 to 3,055,154 _RS14550 Position (kb) 3051 3052 3053Strain fitness (log2 ratio) -1 0 1at 3050.710 kb on + strand, within BWI76_RS14535at 3050.788 kb on + strandat 3050.838 kb on + strandat 3050.875 kb on - strandat 3051.608 kb on + strandat 3051.616 kb on - strandat 3051.749 kb on + strandat 3051.848 kb on + strand, within BWI76_RS14540at 3052.055 kb on + strand, within BWI76_RS14540at 3052.411 kb on + strand, within BWI76_RS14540at 3052.830 kb on + strandat 3052.914 kb on + strand, within BWI76_RS14545at 3052.948 kb on + strand, within BWI76_RS14545at 3053.344 kb on + strand, within BWI76_RS14545at 3053.384 kb on - strand, within BWI76_RS14545at 3053.562 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x
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3,050,710 + BWI76_RS14535 0.90 +0.6
3,050,788 + -0.3
3,050,838 + +0.6
3,050,875 - -1.2
3,051,608 + -0.1
3,051,616 - -1.0
3,051,749 + -0.7
3,051,848 + BWI76_RS14540 0.16 -0.9
3,052,055 + BWI76_RS14540 0.35 -0.4
3,052,411 + BWI76_RS14540 0.67 -0.8
3,052,830 + +0.2
3,052,914 + BWI76_RS14545 0.18 -0.0
3,052,948 + BWI76_RS14545 0.22 -0.2
3,053,344 + BWI76_RS14545 0.71 -0.4
3,053,384 - BWI76_RS14545 0.76 -0.2
3,053,562 + -0.5

Or see this region's nucleotide sequence