Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14200

Experiment: RCH2_defined_glucose with MS_media 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14195 and BWI76_RS14200 overlap by 4 nucleotidesBWI76_RS14200 and BWI76_RS14205 are separated by 13 nucleotides BWI76_RS14195: BWI76_RS14195 - LysR family transcriptional regulator, at 2,970,615 to 2,971,838 _RS14195 BWI76_RS14200: BWI76_RS14200 - protocatechuate 4,5-dioxygenase, at 2,971,835 to 2,973,097 _RS14200 BWI76_RS14205: BWI76_RS14205 - aromatic acid/H+ symport family MFS transporter, at 2,973,111 to 2,974,480 _RS14205 Position (kb) 2971 2972 2973 2974Strain fitness (log2 ratio) -2 -1 0 1at 2970.835 kb on - strand, within BWI76_RS14195at 2970.877 kb on - strand, within BWI76_RS14195at 2970.882 kb on - strand, within BWI76_RS14195at 2970.892 kb on + strand, within BWI76_RS14195at 2970.906 kb on + strand, within BWI76_RS14195at 2970.923 kb on + strand, within BWI76_RS14195at 2970.931 kb on - strand, within BWI76_RS14195at 2970.988 kb on + strand, within BWI76_RS14195at 2971.011 kb on - strand, within BWI76_RS14195at 2971.016 kb on - strand, within BWI76_RS14195at 2971.018 kb on + strand, within BWI76_RS14195at 2971.026 kb on - strand, within BWI76_RS14195at 2971.047 kb on - strand, within BWI76_RS14195at 2971.216 kb on - strand, within BWI76_RS14195at 2971.391 kb on + strand, within BWI76_RS14195at 2971.480 kb on + strand, within BWI76_RS14195at 2971.571 kb on - strand, within BWI76_RS14195at 2971.627 kb on + strand, within BWI76_RS14195at 2972.042 kb on + strand, within BWI76_RS14200at 2972.572 kb on - strand, within BWI76_RS14200at 2972.596 kb on + strand, within BWI76_RS14200at 2972.635 kb on - strand, within BWI76_RS14200at 2973.711 kb on + strand, within BWI76_RS14205at 2973.745 kb on + strand, within BWI76_RS14205at 2973.745 kb on + strand, within BWI76_RS14205at 2973.745 kb on + strand, within BWI76_RS14205at 2973.745 kb on + strand, within BWI76_RS14205at 2973.753 kb on - strand, within BWI76_RS14205at 2973.753 kb on - strand, within BWI76_RS14205at 2973.753 kb on - strand, within BWI76_RS14205at 2973.753 kb on - strand, within BWI76_RS14205at 2973.866 kb on + strand, within BWI76_RS14205at 2973.997 kb on - strand, within BWI76_RS14205at 2974.002 kb on - strand, within BWI76_RS14205at 2974.027 kb on + strand, within BWI76_RS14205at 2974.056 kb on + strand, within BWI76_RS14205at 2974.056 kb on + strand, within BWI76_RS14205

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x
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2,970,835 - BWI76_RS14195 0.18 +0.2
2,970,877 - BWI76_RS14195 0.21 -0.4
2,970,882 - BWI76_RS14195 0.22 -0.8
2,970,892 + BWI76_RS14195 0.23 -0.4
2,970,906 + BWI76_RS14195 0.24 +0.4
2,970,923 + BWI76_RS14195 0.25 +0.5
2,970,931 - BWI76_RS14195 0.26 -0.2
2,970,988 + BWI76_RS14195 0.30 -0.6
2,971,011 - BWI76_RS14195 0.32 -0.2
2,971,016 - BWI76_RS14195 0.33 -1.6
2,971,018 + BWI76_RS14195 0.33 +0.2
2,971,026 - BWI76_RS14195 0.34 -0.5
2,971,047 - BWI76_RS14195 0.35 +0.2
2,971,216 - BWI76_RS14195 0.49 +0.6
2,971,391 + BWI76_RS14195 0.63 +0.4
2,971,480 + BWI76_RS14195 0.71 -0.6
2,971,571 - BWI76_RS14195 0.78 -0.3
2,971,627 + BWI76_RS14195 0.83 -0.5
2,972,042 + BWI76_RS14200 0.16 -0.3
2,972,572 - BWI76_RS14200 0.58 +0.2
2,972,596 + BWI76_RS14200 0.60 +0.3
2,972,635 - BWI76_RS14200 0.63 +0.9
2,973,711 + BWI76_RS14205 0.44 -0.4
2,973,745 + BWI76_RS14205 0.46 +0.2
2,973,745 + BWI76_RS14205 0.46 -2.0
2,973,745 + BWI76_RS14205 0.46 +0.3
2,973,745 + BWI76_RS14205 0.46 -0.4
2,973,753 - BWI76_RS14205 0.47 -0.2
2,973,753 - BWI76_RS14205 0.47 -0.5
2,973,753 - BWI76_RS14205 0.47 +0.2
2,973,753 - BWI76_RS14205 0.47 +0.0
2,973,866 + BWI76_RS14205 0.55 +0.2
2,973,997 - BWI76_RS14205 0.65 +0.3
2,974,002 - BWI76_RS14205 0.65 -0.8
2,974,027 + BWI76_RS14205 0.67 +0.0
2,974,056 + BWI76_RS14205 0.69 +0.1
2,974,056 + BWI76_RS14205 0.69 -0.2

Or see this region's nucleotide sequence