Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS13645

Experiment: RCH2_defined_glucose with MS_media 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS13640 and BWI76_RS13645 are separated by 76 nucleotidesBWI76_RS13645 and BWI76_RS13650 are separated by 129 nucleotidesBWI76_RS13650 and BWI76_RS13655 overlap by 4 nucleotides BWI76_RS13640: BWI76_RS13640 - binding-protein-dependent transport system inner membrane protein, at 2,842,750 to 2,844,405 _RS13640 BWI76_RS13645: BWI76_RS13645 - N-hydroxyarylamine O-acetyltransferase, at 2,844,482 to 2,845,327 _RS13645 BWI76_RS13650: BWI76_RS13650 - respiratory nitrate reductase subunit gamma, at 2,845,457 to 2,846,137 _RS13650 BWI76_RS13655: BWI76_RS13655 - nitrate reductase molybdenum cofactor assembly chaperone, at 2,846,134 to 2,846,829 _RS13655 Position (kb) 2844 2845 2846Strain fitness (log2 ratio) -2 -1 0 1at 2843.913 kb on + strand, within BWI76_RS13640at 2843.990 kb on - strand, within BWI76_RS13640at 2844.089 kb on - strand, within BWI76_RS13640at 2844.310 kb on + strandat 2844.531 kb on + strandat 2844.714 kb on - strand, within BWI76_RS13645at 2844.732 kb on + strand, within BWI76_RS13645at 2844.986 kb on - strand, within BWI76_RS13645at 2845.315 kb on - strandat 2845.477 kb on + strandat 2845.479 kb on + strandat 2845.512 kb on + strandat 2845.653 kb on - strand, within BWI76_RS13650at 2846.090 kb on + strandat 2846.096 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x
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2,843,913 + BWI76_RS13640 0.70 +0.6
2,843,990 - BWI76_RS13640 0.75 +0.1
2,844,089 - BWI76_RS13640 0.81 -0.2
2,844,310 + -0.4
2,844,531 + +0.1
2,844,714 - BWI76_RS13645 0.27 -1.1
2,844,732 + BWI76_RS13645 0.30 -0.5
2,844,986 - BWI76_RS13645 0.60 -2.0
2,845,315 - -0.3
2,845,477 + +0.6
2,845,479 + -0.6
2,845,512 + -0.4
2,845,653 - BWI76_RS13650 0.29 +0.4
2,846,090 + -1.1
2,846,096 + +0.3

Or see this region's nucleotide sequence