Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13640 and BWI76_RS13645 are separated by 76 nucleotides BWI76_RS13645 and BWI76_RS13650 are separated by 129 nucleotides BWI76_RS13650 and BWI76_RS13655 overlap by 4 nucleotides
BWI76_RS13640: BWI76_RS13640 - binding-protein-dependent transport system inner membrane protein, at 2,842,750 to 2,844,405
_RS13640
BWI76_RS13645: BWI76_RS13645 - N-hydroxyarylamine O-acetyltransferase, at 2,844,482 to 2,845,327
_RS13645
BWI76_RS13650: BWI76_RS13650 - respiratory nitrate reductase subunit gamma, at 2,845,457 to 2,846,137
_RS13650
BWI76_RS13655: BWI76_RS13655 - nitrate reductase molybdenum cofactor assembly chaperone, at 2,846,134 to 2,846,829
_RS13655
Position (kb)
2844
2845
2846 Strain fitness (log2 ratio)
-2
-1
0
1 at 2843.913 kb on + strand, within BWI76_RS13640 at 2843.990 kb on - strand, within BWI76_RS13640 at 2844.089 kb on - strand, within BWI76_RS13640 at 2844.310 kb on + strand at 2844.531 kb on + strand at 2844.714 kb on - strand, within BWI76_RS13645 at 2844.732 kb on + strand, within BWI76_RS13645 at 2844.986 kb on - strand, within BWI76_RS13645 at 2845.315 kb on - strand at 2845.477 kb on + strand at 2845.479 kb on + strand at 2845.512 kb on + strand at 2845.653 kb on - strand, within BWI76_RS13650 at 2846.090 kb on + strand at 2846.096 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 2,843,913 + BWI76_RS13640 0.70 +0.6 2,843,990 - BWI76_RS13640 0.75 +0.1 2,844,089 - BWI76_RS13640 0.81 -0.2 2,844,310 + -0.4 2,844,531 + +0.1 2,844,714 - BWI76_RS13645 0.27 -1.1 2,844,732 + BWI76_RS13645 0.30 -0.5 2,844,986 - BWI76_RS13645 0.60 -2.0 2,845,315 - -0.3 2,845,477 + +0.6 2,845,479 + -0.6 2,845,512 + -0.4 2,845,653 - BWI76_RS13650 0.29 +0.4 2,846,090 + -1.1 2,846,096 + +0.3
Or see this region's nucleotide sequence