Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS12940

Experiment: RCH2_defined_glucose with MS_media 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS12935 and BWI76_RS12940 are separated by 77 nucleotidesBWI76_RS12940 and BWI76_RS12945 are separated by 57 nucleotidesBWI76_RS12945 and BWI76_RS12950 are separated by 334 nucleotides BWI76_RS12935: BWI76_RS12935 - fusaric acid resistance protein, at 2,693,919 to 2,695,958 _RS12935 BWI76_RS12940: BWI76_RS12940 - AraC family transcriptional regulator, at 2,696,036 to 2,696,857 _RS12940 BWI76_RS12945: BWI76_RS12945 - AraC family transcriptional regulator, at 2,696,915 to 2,697,310 _RS12945 BWI76_RS12950: BWI76_RS12950 - acriflavin resistance protein AcrA, at 2,697,645 to 2,698,820 _RS12950 Position (kb) 2696 2697Strain fitness (log2 ratio) -1 0 1at 2695.368 kb on - strand, within BWI76_RS12935at 2695.368 kb on - strand, within BWI76_RS12935at 2695.485 kb on + strand, within BWI76_RS12935at 2695.493 kb on - strand, within BWI76_RS12935at 2695.854 kb on + strandat 2695.885 kb on + strandat 2695.885 kb on + strandat 2696.060 kb on - strandat 2696.181 kb on + strand, within BWI76_RS12940at 2696.414 kb on + strand, within BWI76_RS12940at 2696.777 kb on - strandat 2696.859 kb on - strandat 2696.859 kb on - strandat 2697.835 kb on - strand, within BWI76_RS12950

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x
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2,695,368 - BWI76_RS12935 0.71 -0.5
2,695,368 - BWI76_RS12935 0.71 -0.4
2,695,485 + BWI76_RS12935 0.77 -0.6
2,695,493 - BWI76_RS12935 0.77 +0.1
2,695,854 + -0.3
2,695,885 + +1.3
2,695,885 + -0.0
2,696,060 - -0.1
2,696,181 + BWI76_RS12940 0.18 -0.9
2,696,414 + BWI76_RS12940 0.46 -0.9
2,696,777 - -0.3
2,696,859 - +0.6
2,696,859 - -0.3
2,697,835 - BWI76_RS12950 0.16 -0.7

Or see this region's nucleotide sequence