Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12185 and BWI76_RS12190 are separated by 7 nucleotides BWI76_RS12190 and BWI76_RS12195 are separated by 2 nucleotides BWI76_RS12195 and BWI76_RS12200 overlap by 50 nucleotides BWI76_RS12200 and BWI76_RS12205 overlap by 4 nucleotides
BWI76_RS12185: BWI76_RS12185 - hypothetical protein, at 2,552,514 to 2,552,792
_RS12185
BWI76_RS12190: BWI76_RS12190 - glycoside hydrolase family protein, at 2,552,800 to 2,553,429
_RS12190
BWI76_RS12195: BWI76_RS12195 - hypothetical protein, at 2,553,432 to 2,553,707
_RS12195
BWI76_RS12200: BWI76_RS12200 - hypothetical protein, at 2,553,658 to 2,553,852
_RS12200
BWI76_RS12205: BWI76_RS12205 - hypothetical protein, at 2,553,849 to 2,554,133
_RS12205
Position (kb)
2553
2554 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2553.015 kb on + strand at 2553.033 kb on + strand, within BWI76_RS12190 at 2553.033 kb on + strand, within BWI76_RS12190 at 2553.036 kb on - strand, within BWI76_RS12190 at 2553.036 kb on - strand, within BWI76_RS12190 at 2553.045 kb on + strand at 2553.047 kb on + strand, within BWI76_RS12190 at 2553.054 kb on + strand at 2553.081 kb on - strand, within BWI76_RS12190 at 2553.140 kb on - strand, within BWI76_RS12190 at 2553.140 kb on - strand, within BWI76_RS12190 at 2553.160 kb on - strand, within BWI76_RS12190 at 2553.160 kb on - strand, within BWI76_RS12190 at 2553.287 kb on - strand, within BWI76_RS12190 at 2553.287 kb on - strand, within BWI76_RS12190 at 2553.289 kb on + strand, within BWI76_RS12190 at 2553.400 kb on - strand at 2553.496 kb on + strand, within BWI76_RS12195 at 2553.504 kb on - strand, within BWI76_RS12195 at 2553.553 kb on - strand, within BWI76_RS12195 at 2553.578 kb on - strand, within BWI76_RS12195 at 2553.615 kb on - strand at 2553.627 kb on - strand, within BWI76_RS12195 at 2553.719 kb on + strand, within BWI76_RS12200 at 2553.751 kb on - strand, within BWI76_RS12200 at 2553.856 kb on + strand at 2553.879 kb on + strand, within BWI76_RS12205 at 2553.968 kb on + strand, within BWI76_RS12205 at 2554.104 kb on + strand, within BWI76_RS12205 at 2554.127 kb on + strand at 2554.152 kb on + strand at 2554.160 kb on - strand at 2554.160 kb on - strand at 2554.160 kb on - strand at 2554.237 kb on + strand at 2554.245 kb on - strand at 2554.255 kb on - strand at 2554.279 kb on + strand at 2554.287 kb on - strand at 2554.287 kb on - strand at 2554.293 kb on - strand at 2554.294 kb on + strand at 2554.303 kb on + strand at 2554.308 kb on + strand at 2554.391 kb on + strand at 2554.399 kb on - strand at 2554.406 kb on - strand at 2554.406 kb on - strand at 2554.469 kb on - strand at 2554.505 kb on - strand at 2554.511 kb on - strand at 2554.513 kb on - strand at 2554.513 kb on - strand at 2554.516 kb on + strand at 2554.516 kb on + strand at 2554.516 kb on + strand at 2554.516 kb on + strand at 2554.516 kb on + strand at 2554.533 kb on - strand at 2554.543 kb on - strand at 2554.565 kb on + strand at 2554.571 kb on + strand at 2554.580 kb on + strand at 2554.580 kb on + strand at 2554.588 kb on - strand at 2554.689 kb on + strand at 2554.690 kb on + strand at 2554.694 kb on + strand at 2554.694 kb on + strand at 2554.699 kb on + strand at 2554.720 kb on - strand at 2554.730 kb on - strand at 2554.744 kb on + strand at 2554.798 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 2,553,015 + +0.6 2,553,033 + BWI76_RS12190 0.37 -0.4 2,553,033 + BWI76_RS12190 0.37 +0.0 2,553,036 - BWI76_RS12190 0.37 -1.0 2,553,036 - BWI76_RS12190 0.37 -0.2 2,553,045 + +1.7 2,553,047 + BWI76_RS12190 0.39 +0.5 2,553,054 + +2.5 2,553,081 - BWI76_RS12190 0.45 -0.1 2,553,140 - BWI76_RS12190 0.54 -0.4 2,553,140 - BWI76_RS12190 0.54 -0.4 2,553,160 - BWI76_RS12190 0.57 -0.6 2,553,160 - BWI76_RS12190 0.57 -0.3 2,553,287 - BWI76_RS12190 0.77 +0.0 2,553,287 - BWI76_RS12190 0.77 -2.3 2,553,289 + BWI76_RS12190 0.78 -0.5 2,553,400 - -0.4 2,553,496 + BWI76_RS12195 0.23 -0.4 2,553,504 - BWI76_RS12195 0.26 -1.0 2,553,553 - BWI76_RS12195 0.44 -0.3 2,553,578 - BWI76_RS12195 0.53 +0.1 2,553,615 - +0.7 2,553,627 - BWI76_RS12195 0.71 -0.5 2,553,719 + BWI76_RS12200 0.31 -1.4 2,553,751 - BWI76_RS12200 0.48 -0.2 2,553,856 + +0.1 2,553,879 + BWI76_RS12205 0.11 +0.3 2,553,968 + BWI76_RS12205 0.42 +0.2 2,554,104 + BWI76_RS12205 0.89 +0.1 2,554,127 + -0.9 2,554,152 + -0.9 2,554,160 - +0.9 2,554,160 - +0.5 2,554,160 - -0.5 2,554,237 + -0.1 2,554,245 - -0.5 2,554,255 - -0.2 2,554,279 + -0.0 2,554,287 - -0.0 2,554,287 - -0.4 2,554,293 - -0.6 2,554,294 + -0.0 2,554,303 + -0.8 2,554,308 + -0.1 2,554,391 + +0.8 2,554,399 - +0.8 2,554,406 - -0.9 2,554,406 - -0.1 2,554,469 - -0.2 2,554,505 - -0.8 2,554,511 - -0.6 2,554,513 - -0.2 2,554,513 - +0.5 2,554,516 + -0.2 2,554,516 + -0.9 2,554,516 + +0.1 2,554,516 + -0.4 2,554,516 + -0.5 2,554,533 - +0.4 2,554,543 - +2.0 2,554,565 + -0.1 2,554,571 + -0.6 2,554,580 + -0.9 2,554,580 + -0.1 2,554,588 - -1.8 2,554,689 + -0.2 2,554,690 + -0.6 2,554,694 + +0.3 2,554,694 + -0.5 2,554,699 + -0.3 2,554,720 - +0.1 2,554,730 - +0.5 2,554,744 + -0.3 2,554,798 + +0.3
Or see this region's nucleotide sequence