Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10365

Experiment: RCH2_defined_glucose with MS_media 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10360 and BWI76_RS10365 are separated by 433 nucleotidesBWI76_RS10365 and BWI76_RS10370 are separated by 15 nucleotidesBWI76_RS10370 and BWI76_RS10375 are separated by 16 nucleotides BWI76_RS10355: BWI76_RS10355 - spermidine/putrescine ABC transporter, at 2,192,997 to 2,194,092 _RS10355 BWI76_RS10360: BWI76_RS10360 - TetR family transcriptional regulator, at 2,194,068 to 2,194,646 _RS10360 BWI76_RS10365: BWI76_RS10365 - class IV aminotransferase, at 2,195,080 to 2,196,045 _RS10365 BWI76_RS10370: BWI76_RS10370 - ABC transporter-like protein, at 2,196,061 to 2,196,840 _RS10370 BWI76_RS10375: BWI76_RS10375 - polar amino acid ABC transporter inner membrane subunit, at 2,196,857 to 2,197,522 _RS10375 Position (kb) 2195 2196 2197Strain fitness (log2 ratio) -1 0 1at 2194.443 kb on + strand, within BWI76_RS10360at 2194.673 kb on - strandat 2194.742 kb on + strandat 2194.883 kb on + strandat 2194.967 kb on + strandat 2195.026 kb on + strandat 2195.139 kb on - strandat 2195.193 kb on + strand, within BWI76_RS10365at 2195.357 kb on - strand, within BWI76_RS10365at 2195.586 kb on + strand, within BWI76_RS10365at 2195.586 kb on + strand, within BWI76_RS10365at 2195.586 kb on + strand, within BWI76_RS10365at 2196.102 kb on - strandat 2196.210 kb on + strand, within BWI76_RS10370at 2196.293 kb on - strand, within BWI76_RS10370at 2196.369 kb on + strand, within BWI76_RS10370at 2196.452 kb on - strand, within BWI76_RS10370at 2196.630 kb on + strand, within BWI76_RS10370at 2196.630 kb on + strand, within BWI76_RS10370at 2196.630 kb on + strand, within BWI76_RS10370at 2196.638 kb on - strand, within BWI76_RS10370at 2196.638 kb on - strand, within BWI76_RS10370at 2196.638 kb on - strand, within BWI76_RS10370at 2196.844 kb on - strandat 2196.909 kb on + strandat 2196.924 kb on + strand, within BWI76_RS10375

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x
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2,194,443 + BWI76_RS10360 0.65 -0.4
2,194,673 - -0.3
2,194,742 + -0.1
2,194,883 + +0.2
2,194,967 + +0.7
2,195,026 + -0.1
2,195,139 - +0.1
2,195,193 + BWI76_RS10365 0.12 -0.4
2,195,357 - BWI76_RS10365 0.29 -0.4
2,195,586 + BWI76_RS10365 0.52 -0.8
2,195,586 + BWI76_RS10365 0.52 -1.5
2,195,586 + BWI76_RS10365 0.52 -0.7
2,196,102 - -0.6
2,196,210 + BWI76_RS10370 0.19 -1.1
2,196,293 - BWI76_RS10370 0.30 -0.6
2,196,369 + BWI76_RS10370 0.39 -0.3
2,196,452 - BWI76_RS10370 0.50 +0.6
2,196,630 + BWI76_RS10370 0.73 -0.0
2,196,630 + BWI76_RS10370 0.73 +0.4
2,196,630 + BWI76_RS10370 0.73 -1.5
2,196,638 - BWI76_RS10370 0.74 -0.3
2,196,638 - BWI76_RS10370 0.74 +0.8
2,196,638 - BWI76_RS10370 0.74 +0.8
2,196,844 - -0.2
2,196,909 + -1.1
2,196,924 + BWI76_RS10375 0.10 +0.1

Or see this region's nucleotide sequence