Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10360 and BWI76_RS10365 are separated by 433 nucleotides BWI76_RS10365 and BWI76_RS10370 are separated by 15 nucleotides BWI76_RS10370 and BWI76_RS10375 are separated by 16 nucleotides
BWI76_RS10355: BWI76_RS10355 - spermidine/putrescine ABC transporter, at 2,192,997 to 2,194,092
_RS10355
BWI76_RS10360: BWI76_RS10360 - TetR family transcriptional regulator, at 2,194,068 to 2,194,646
_RS10360
BWI76_RS10365: BWI76_RS10365 - class IV aminotransferase, at 2,195,080 to 2,196,045
_RS10365
BWI76_RS10370: BWI76_RS10370 - ABC transporter-like protein, at 2,196,061 to 2,196,840
_RS10370
BWI76_RS10375: BWI76_RS10375 - polar amino acid ABC transporter inner membrane subunit, at 2,196,857 to 2,197,522
_RS10375
Position (kb)
2195
2196
2197 Strain fitness (log2 ratio)
-1
0
1 at 2194.443 kb on + strand, within BWI76_RS10360 at 2194.673 kb on - strand at 2194.742 kb on + strand at 2194.883 kb on + strand at 2194.967 kb on + strand at 2195.026 kb on + strand at 2195.139 kb on - strand at 2195.193 kb on + strand, within BWI76_RS10365 at 2195.357 kb on - strand, within BWI76_RS10365 at 2195.586 kb on + strand, within BWI76_RS10365 at 2195.586 kb on + strand, within BWI76_RS10365 at 2195.586 kb on + strand, within BWI76_RS10365 at 2196.102 kb on - strand at 2196.210 kb on + strand, within BWI76_RS10370 at 2196.293 kb on - strand, within BWI76_RS10370 at 2196.369 kb on + strand, within BWI76_RS10370 at 2196.452 kb on - strand, within BWI76_RS10370 at 2196.630 kb on + strand, within BWI76_RS10370 at 2196.630 kb on + strand, within BWI76_RS10370 at 2196.630 kb on + strand, within BWI76_RS10370 at 2196.638 kb on - strand, within BWI76_RS10370 at 2196.638 kb on - strand, within BWI76_RS10370 at 2196.638 kb on - strand, within BWI76_RS10370 at 2196.844 kb on - strand at 2196.909 kb on + strand at 2196.924 kb on + strand, within BWI76_RS10375
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 2,194,443 + BWI76_RS10360 0.65 -0.4 2,194,673 - -0.3 2,194,742 + -0.1 2,194,883 + +0.2 2,194,967 + +0.7 2,195,026 + -0.1 2,195,139 - +0.1 2,195,193 + BWI76_RS10365 0.12 -0.4 2,195,357 - BWI76_RS10365 0.29 -0.4 2,195,586 + BWI76_RS10365 0.52 -0.8 2,195,586 + BWI76_RS10365 0.52 -1.5 2,195,586 + BWI76_RS10365 0.52 -0.7 2,196,102 - -0.6 2,196,210 + BWI76_RS10370 0.19 -1.1 2,196,293 - BWI76_RS10370 0.30 -0.6 2,196,369 + BWI76_RS10370 0.39 -0.3 2,196,452 - BWI76_RS10370 0.50 +0.6 2,196,630 + BWI76_RS10370 0.73 -0.0 2,196,630 + BWI76_RS10370 0.73 +0.4 2,196,630 + BWI76_RS10370 0.73 -1.5 2,196,638 - BWI76_RS10370 0.74 -0.3 2,196,638 - BWI76_RS10370 0.74 +0.8 2,196,638 - BWI76_RS10370 0.74 +0.8 2,196,844 - -0.2 2,196,909 + -1.1 2,196,924 + BWI76_RS10375 0.10 +0.1
Or see this region's nucleotide sequence