Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS09715

Experiment: RCH2_defined_glucose with MS_media 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS09705 and BWI76_RS09710 overlap by 1 nucleotidesBWI76_RS09710 and BWI76_RS09715 are separated by 12 nucleotidesBWI76_RS09715 and BWI76_RS09720 are separated by 17 nucleotidesBWI76_RS09720 and BWI76_RS09725 are separated by 122 nucleotides BWI76_RS09705: BWI76_RS09705 - transketolase, at 2,034,570 to 2,035,481 _RS09705 BWI76_RS09710: BWI76_RS09710 - transketolase, at 2,035,481 to 2,036,278 _RS09710 BWI76_RS09715: BWI76_RS09715 - hypothetical protein, at 2,036,291 to 2,037,556 _RS09715 BWI76_RS09720: BWI76_RS09720 - PTS ascorbate transporter subunit IIB, at 2,037,574 to 2,037,849 _RS09720 BWI76_RS09725: BWI76_RS09725 - MurR/RpiR family transcriptional regulator, at 2,037,972 to 2,038,829 _RS09725 Position (kb) 2036 2037 2038Strain fitness (log2 ratio) -1 0 1 2at 2035.382 kb on + strand, within BWI76_RS09705at 2035.448 kb on + strandat 2035.605 kb on + strand, within BWI76_RS09710at 2035.965 kb on + strand, within BWI76_RS09710at 2036.065 kb on + strand, within BWI76_RS09710at 2036.263 kb on - strandat 2036.407 kb on + strandat 2036.608 kb on - strand, within BWI76_RS09715at 2036.608 kb on - strand, within BWI76_RS09715at 2036.663 kb on - strand, within BWI76_RS09715at 2036.895 kb on + strand, within BWI76_RS09715at 2036.998 kb on - strand, within BWI76_RS09715at 2037.174 kb on + strand, within BWI76_RS09715at 2037.228 kb on - strand, within BWI76_RS09715at 2037.605 kb on + strand, within BWI76_RS09720at 2037.623 kb on - strand, within BWI76_RS09720at 2037.628 kb on - strand, within BWI76_RS09720at 2037.728 kb on + strand, within BWI76_RS09720at 2037.855 kb on + strandat 2038.181 kb on + strand, within BWI76_RS09725at 2038.544 kb on + strand, within BWI76_RS09725

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x
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2,035,382 + BWI76_RS09705 0.89 +1.4
2,035,448 + -0.4
2,035,605 + BWI76_RS09710 0.16 -0.1
2,035,965 + BWI76_RS09710 0.61 +0.0
2,036,065 + BWI76_RS09710 0.73 +0.6
2,036,263 - -0.8
2,036,407 + -0.1
2,036,608 - BWI76_RS09715 0.25 +0.3
2,036,608 - BWI76_RS09715 0.25 +0.2
2,036,663 - BWI76_RS09715 0.29 +0.5
2,036,895 + BWI76_RS09715 0.48 +0.3
2,036,998 - BWI76_RS09715 0.56 +1.9
2,037,174 + BWI76_RS09715 0.70 -0.5
2,037,228 - BWI76_RS09715 0.74 -0.2
2,037,605 + BWI76_RS09720 0.11 -0.5
2,037,623 - BWI76_RS09720 0.18 -0.2
2,037,628 - BWI76_RS09720 0.20 -0.6
2,037,728 + BWI76_RS09720 0.56 -0.0
2,037,855 + -0.7
2,038,181 + BWI76_RS09725 0.24 +0.4
2,038,544 + BWI76_RS09725 0.67 -0.9

Or see this region's nucleotide sequence