Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01030 and BWI76_RS01035 are separated by 18 nucleotides BWI76_RS01035 and BWI76_RS01040 are separated by 221 nucleotides
BWI76_RS01030: BWI76_RS01030 - cytosine/purine, uracil, thiamine, allantoin permease family protein, at 210,461 to 211,876
_RS01030
BWI76_RS01035: BWI76_RS01035 - ADP-ribosylation/crystallin J1, at 211,895 to 212,911
_RS01035
BWI76_RS01040: BWI76_RS01040 - transcriptional regulator, at 213,133 to 213,918
_RS01040
Position (kb)
211
212
213 Strain fitness (log2 ratio)
-1
0
1
2 at 211.104 kb on + strand, within BWI76_RS01030 at 211.256 kb on - strand, within BWI76_RS01030 at 211.441 kb on + strand, within BWI76_RS01030 at 211.482 kb on - strand, within BWI76_RS01030 at 211.626 kb on + strand, within BWI76_RS01030 at 211.856 kb on + strand at 211.886 kb on - strand at 211.990 kb on + strand at 212.493 kb on + strand, within BWI76_RS01035 at 212.568 kb on - strand, within BWI76_RS01035 at 212.690 kb on + strand, within BWI76_RS01035 at 212.699 kb on + strand, within BWI76_RS01035 at 212.703 kb on - strand, within BWI76_RS01035 at 212.707 kb on - strand, within BWI76_RS01035 at 212.815 kb on + strand at 212.815 kb on + strand at 212.823 kb on + strand at 213.173 kb on + strand at 213.192 kb on + strand at 213.192 kb on + strand at 213.192 kb on + strand at 213.204 kb on + strand at 213.290 kb on + strand, within BWI76_RS01040 at 213.298 kb on - strand, within BWI76_RS01040 at 213.315 kb on + strand, within BWI76_RS01040 at 213.315 kb on + strand, within BWI76_RS01040 at 213.336 kb on + strand, within BWI76_RS01040 at 213.398 kb on + strand, within BWI76_RS01040 at 213.398 kb on + strand, within BWI76_RS01040 at 213.398 kb on + strand, within BWI76_RS01040 at 213.406 kb on - strand, within BWI76_RS01040 at 213.406 kb on - strand at 213.406 kb on - strand, within BWI76_RS01040 at 213.527 kb on + strand, within BWI76_RS01040 at 213.556 kb on + strand, within BWI76_RS01040 at 213.556 kb on + strand, within BWI76_RS01040 at 213.561 kb on + strand, within BWI76_RS01040 at 213.562 kb on + strand, within BWI76_RS01040 at 213.696 kb on + strand, within BWI76_RS01040 at 213.770 kb on - strand, within BWI76_RS01040 at 213.782 kb on + strand, within BWI76_RS01040 at 213.803 kb on - strand, within BWI76_RS01040 at 213.815 kb on + strand, within BWI76_RS01040 at 213.821 kb on + strand, within BWI76_RS01040 at 213.821 kb on + strand, within BWI76_RS01040 at 213.829 kb on - strand at 213.864 kb on + strand at 213.870 kb on + strand at 213.878 kb on - strand at 213.887 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 211,104 + BWI76_RS01030 0.45 -0.4 211,256 - BWI76_RS01030 0.56 -0.4 211,441 + BWI76_RS01030 0.69 +0.0 211,482 - BWI76_RS01030 0.72 -1.1 211,626 + BWI76_RS01030 0.82 -0.2 211,856 + +0.1 211,886 - -0.4 211,990 + +0.1 212,493 + BWI76_RS01035 0.59 +2.7 212,568 - BWI76_RS01035 0.66 +0.2 212,690 + BWI76_RS01035 0.78 +0.7 212,699 + BWI76_RS01035 0.79 -0.2 212,703 - BWI76_RS01035 0.79 +0.2 212,707 - BWI76_RS01035 0.80 -0.3 212,815 + +0.1 212,815 + -0.8 212,823 + -1.3 213,173 + -0.9 213,192 + -0.6 213,192 + +0.1 213,192 + +0.3 213,204 + -0.3 213,290 + BWI76_RS01040 0.20 -0.4 213,298 - BWI76_RS01040 0.21 -1.5 213,315 + BWI76_RS01040 0.23 -0.3 213,315 + BWI76_RS01040 0.23 -0.8 213,336 + BWI76_RS01040 0.26 -1.1 213,398 + BWI76_RS01040 0.34 -1.4 213,398 + BWI76_RS01040 0.34 -0.3 213,398 + BWI76_RS01040 0.34 -0.6 213,406 - BWI76_RS01040 0.35 -0.0 213,406 - -1.3 213,406 - BWI76_RS01040 0.35 -0.2 213,527 + BWI76_RS01040 0.50 -0.2 213,556 + BWI76_RS01040 0.54 -0.2 213,556 + BWI76_RS01040 0.54 +0.7 213,561 + BWI76_RS01040 0.54 -0.1 213,562 + BWI76_RS01040 0.55 -0.3 213,696 + BWI76_RS01040 0.72 +0.2 213,770 - BWI76_RS01040 0.81 +0.3 213,782 + BWI76_RS01040 0.83 -0.2 213,803 - BWI76_RS01040 0.85 -0.1 213,815 + BWI76_RS01040 0.87 -0.1 213,821 + BWI76_RS01040 0.88 -0.2 213,821 + BWI76_RS01040 0.88 -0.6 213,829 - -0.8 213,864 + +0.2 213,870 + -0.1 213,878 - -0.3 213,887 + -0.2
Or see this region's nucleotide sequence