Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01035

Experiment: RCH2_defined_glucose with MS_media 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01030 and BWI76_RS01035 are separated by 18 nucleotidesBWI76_RS01035 and BWI76_RS01040 are separated by 221 nucleotides BWI76_RS01030: BWI76_RS01030 - cytosine/purine, uracil, thiamine, allantoin permease family protein, at 210,461 to 211,876 _RS01030 BWI76_RS01035: BWI76_RS01035 - ADP-ribosylation/crystallin J1, at 211,895 to 212,911 _RS01035 BWI76_RS01040: BWI76_RS01040 - transcriptional regulator, at 213,133 to 213,918 _RS01040 Position (kb) 211 212 213Strain fitness (log2 ratio) -1 0 1 2at 211.104 kb on + strand, within BWI76_RS01030at 211.256 kb on - strand, within BWI76_RS01030at 211.441 kb on + strand, within BWI76_RS01030at 211.482 kb on - strand, within BWI76_RS01030at 211.626 kb on + strand, within BWI76_RS01030at 211.856 kb on + strandat 211.886 kb on - strandat 211.990 kb on + strandat 212.493 kb on + strand, within BWI76_RS01035at 212.568 kb on - strand, within BWI76_RS01035at 212.690 kb on + strand, within BWI76_RS01035at 212.699 kb on + strand, within BWI76_RS01035at 212.703 kb on - strand, within BWI76_RS01035at 212.707 kb on - strand, within BWI76_RS01035at 212.815 kb on + strandat 212.815 kb on + strandat 212.823 kb on + strandat 213.173 kb on + strandat 213.192 kb on + strandat 213.192 kb on + strandat 213.192 kb on + strandat 213.204 kb on + strandat 213.290 kb on + strand, within BWI76_RS01040at 213.298 kb on - strand, within BWI76_RS01040at 213.315 kb on + strand, within BWI76_RS01040at 213.315 kb on + strand, within BWI76_RS01040at 213.336 kb on + strand, within BWI76_RS01040at 213.398 kb on + strand, within BWI76_RS01040at 213.398 kb on + strand, within BWI76_RS01040at 213.398 kb on + strand, within BWI76_RS01040at 213.406 kb on - strand, within BWI76_RS01040at 213.406 kb on - strandat 213.406 kb on - strand, within BWI76_RS01040at 213.527 kb on + strand, within BWI76_RS01040at 213.556 kb on + strand, within BWI76_RS01040at 213.556 kb on + strand, within BWI76_RS01040at 213.561 kb on + strand, within BWI76_RS01040at 213.562 kb on + strand, within BWI76_RS01040at 213.696 kb on + strand, within BWI76_RS01040at 213.770 kb on - strand, within BWI76_RS01040at 213.782 kb on + strand, within BWI76_RS01040at 213.803 kb on - strand, within BWI76_RS01040at 213.815 kb on + strand, within BWI76_RS01040at 213.821 kb on + strand, within BWI76_RS01040at 213.821 kb on + strand, within BWI76_RS01040at 213.829 kb on - strandat 213.864 kb on + strandat 213.870 kb on + strandat 213.878 kb on - strandat 213.887 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x
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211,104 + BWI76_RS01030 0.45 -0.4
211,256 - BWI76_RS01030 0.56 -0.4
211,441 + BWI76_RS01030 0.69 +0.0
211,482 - BWI76_RS01030 0.72 -1.1
211,626 + BWI76_RS01030 0.82 -0.2
211,856 + +0.1
211,886 - -0.4
211,990 + +0.1
212,493 + BWI76_RS01035 0.59 +2.7
212,568 - BWI76_RS01035 0.66 +0.2
212,690 + BWI76_RS01035 0.78 +0.7
212,699 + BWI76_RS01035 0.79 -0.2
212,703 - BWI76_RS01035 0.79 +0.2
212,707 - BWI76_RS01035 0.80 -0.3
212,815 + +0.1
212,815 + -0.8
212,823 + -1.3
213,173 + -0.9
213,192 + -0.6
213,192 + +0.1
213,192 + +0.3
213,204 + -0.3
213,290 + BWI76_RS01040 0.20 -0.4
213,298 - BWI76_RS01040 0.21 -1.5
213,315 + BWI76_RS01040 0.23 -0.3
213,315 + BWI76_RS01040 0.23 -0.8
213,336 + BWI76_RS01040 0.26 -1.1
213,398 + BWI76_RS01040 0.34 -1.4
213,398 + BWI76_RS01040 0.34 -0.3
213,398 + BWI76_RS01040 0.34 -0.6
213,406 - BWI76_RS01040 0.35 -0.0
213,406 - -1.3
213,406 - BWI76_RS01040 0.35 -0.2
213,527 + BWI76_RS01040 0.50 -0.2
213,556 + BWI76_RS01040 0.54 -0.2
213,556 + BWI76_RS01040 0.54 +0.7
213,561 + BWI76_RS01040 0.54 -0.1
213,562 + BWI76_RS01040 0.55 -0.3
213,696 + BWI76_RS01040 0.72 +0.2
213,770 - BWI76_RS01040 0.81 +0.3
213,782 + BWI76_RS01040 0.83 -0.2
213,803 - BWI76_RS01040 0.85 -0.1
213,815 + BWI76_RS01040 0.87 -0.1
213,821 + BWI76_RS01040 0.88 -0.2
213,821 + BWI76_RS01040 0.88 -0.6
213,829 - -0.8
213,864 + +0.2
213,870 + -0.1
213,878 - -0.3
213,887 + -0.2

Or see this region's nucleotide sequence