Experiment: SBM - with RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hsdM-2 and SO4266 are separated by 127 nucleotides SO4266 and hsdR-2 are separated by 304 nucleotides
SO4265: hsdM-2 - type I restriction-modification system, M subunit (NCBI ptt file), at 4,441,110 to 4,442,723
hsdM-2
SO4266: SO4266 - conserved hypothetical protein (NCBI ptt file), at 4,442,851 to 4,443,969
SO4266
SO4267: hsdR-2 - type I restriction-modification system, R subunit (NCBI ptt file), at 4,444,274 to 4,447,840
hsdR-2
Position (kb)
4442
4443
4444 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4441.927 kb on - strand, within hsdM-2 at 4442.207 kb on + strand, within hsdM-2 at 4442.207 kb on + strand, within hsdM-2 at 4442.231 kb on + strand, within hsdM-2 at 4442.231 kb on + strand, within hsdM-2 at 4442.238 kb on + strand, within hsdM-2 at 4442.238 kb on + strand, within hsdM-2 at 4442.246 kb on - strand, within hsdM-2 at 4442.246 kb on - strand, within hsdM-2 at 4442.246 kb on - strand, within hsdM-2 at 4442.246 kb on - strand, within hsdM-2 at 4442.254 kb on + strand, within hsdM-2 at 4442.262 kb on - strand, within hsdM-2 at 4442.294 kb on - strand, within hsdM-2 at 4442.360 kb on + strand, within hsdM-2 at 4442.368 kb on - strand, within hsdM-2 at 4442.368 kb on - strand, within hsdM-2 at 4442.388 kb on + strand, within hsdM-2 at 4442.462 kb on + strand, within hsdM-2 at 4442.462 kb on + strand, within hsdM-2 at 4442.470 kb on - strand, within hsdM-2 at 4442.509 kb on - strand, within hsdM-2 at 4442.510 kb on - strand, within hsdM-2 at 4442.565 kb on + strand at 4442.668 kb on + strand at 4442.676 kb on - strand at 4442.760 kb on + strand at 4442.766 kb on + strand at 4442.774 kb on - strand at 4442.805 kb on - strand at 4442.809 kb on + strand at 4442.857 kb on - strand at 4442.872 kb on - strand at 4442.911 kb on - strand at 4442.953 kb on - strand at 4442.963 kb on - strand, within SO4266 at 4442.991 kb on + strand, within SO4266 at 4443.045 kb on - strand, within SO4266 at 4443.168 kb on - strand, within SO4266 at 4443.182 kb on + strand, within SO4266 at 4443.189 kb on + strand at 4443.189 kb on + strand, within SO4266 at 4443.190 kb on - strand, within SO4266 at 4443.190 kb on - strand, within SO4266 at 4443.190 kb on - strand, within SO4266 at 4443.190 kb on - strand, within SO4266 at 4443.285 kb on + strand, within SO4266 at 4443.312 kb on - strand, within SO4266 at 4443.350 kb on - strand, within SO4266 at 4443.355 kb on + strand, within SO4266 at 4443.374 kb on + strand, within SO4266 at 4443.383 kb on + strand, within SO4266 at 4443.383 kb on + strand, within SO4266 at 4443.552 kb on + strand, within SO4266 at 4443.575 kb on + strand, within SO4266 at 4443.584 kb on - strand, within SO4266 at 4443.584 kb on - strand, within SO4266 at 4443.644 kb on - strand at 4443.691 kb on - strand, within SO4266 at 4443.766 kb on + strand, within SO4266 at 4443.774 kb on - strand, within SO4266 at 4443.781 kb on - strand, within SO4266 at 4443.898 kb on - strand at 4443.931 kb on + strand at 4444.024 kb on - strand at 4444.127 kb on + strand at 4444.127 kb on + strand at 4444.135 kb on - strand at 4444.135 kb on - strand at 4444.197 kb on + strand at 4444.217 kb on + strand at 4444.217 kb on + strand at 4444.225 kb on - strand at 4444.232 kb on + strand at 4444.232 kb on + strand at 4444.242 kb on + strand at 4444.251 kb on + strand at 4444.296 kb on + strand at 4444.341 kb on + strand at 4444.349 kb on - strand at 4444.351 kb on - strand at 4444.391 kb on + strand at 4444.443 kb on + strand at 4444.494 kb on + strand at 4444.502 kb on - strand at 4444.502 kb on - strand at 4444.510 kb on + strand at 4444.518 kb on - strand at 4444.518 kb on - strand at 4444.528 kb on - strand at 4444.622 kb on + strand at 4444.630 kb on - strand at 4444.657 kb on + strand, within hsdR-2 at 4444.699 kb on - strand, within hsdR-2 at 4444.703 kb on + strand, within hsdR-2 at 4444.711 kb on - strand, within hsdR-2 at 4444.711 kb on - strand, within hsdR-2 at 4444.752 kb on - strand, within hsdR-2 at 4444.822 kb on + strand, within hsdR-2 at 4444.830 kb on - strand, within hsdR-2 at 4444.830 kb on - strand, within hsdR-2 at 4444.837 kb on + strand, within hsdR-2 at 4444.852 kb on - strand, within hsdR-2 at 4444.856 kb on - strand, within hsdR-2 at 4444.890 kb on + strand, within hsdR-2
Per-strain Table
Position Strand Gene LocusTag Fraction SBM - with RNA protect remove 4,441,927 - hsdM-2 SO4265 0.51 +0.5 4,442,207 + hsdM-2 SO4265 0.68 +0.2 4,442,207 + hsdM-2 SO4265 0.68 +0.8 4,442,231 + hsdM-2 SO4265 0.69 -0.5 4,442,231 + hsdM-2 SO4265 0.69 -0.5 4,442,238 + hsdM-2 SO4265 0.70 -0.1 4,442,238 + hsdM-2 SO4265 0.70 +0.7 4,442,246 - hsdM-2 SO4265 0.70 -0.8 4,442,246 - hsdM-2 SO4265 0.70 -0.1 4,442,246 - hsdM-2 SO4265 0.70 +0.3 4,442,246 - hsdM-2 SO4265 0.70 -0.7 4,442,254 + hsdM-2 SO4265 0.71 -0.5 4,442,262 - hsdM-2 SO4265 0.71 -1.9 4,442,294 - hsdM-2 SO4265 0.73 -0.5 4,442,360 + hsdM-2 SO4265 0.77 -0.5 4,442,368 - hsdM-2 SO4265 0.78 +0.0 4,442,368 - hsdM-2 SO4265 0.78 -0.4 4,442,388 + hsdM-2 SO4265 0.79 +0.3 4,442,462 + hsdM-2 SO4265 0.84 -0.5 4,442,462 + hsdM-2 SO4265 0.84 +1.6 4,442,470 - hsdM-2 SO4265 0.84 +0.6 4,442,509 - hsdM-2 SO4265 0.87 -0.1 4,442,510 - hsdM-2 SO4265 0.87 +0.7 4,442,565 + -0.7 4,442,668 + +0.8 4,442,676 - -0.1 4,442,760 + +0.7 4,442,766 + +0.7 4,442,774 - +0.0 4,442,805 - -0.3 4,442,809 + +0.5 4,442,857 - +0.9 4,442,872 - -0.2 4,442,911 - +1.0 4,442,953 - +1.5 4,442,963 - SO4266 0.10 -0.1 4,442,991 + SO4266 0.13 +1.3 4,443,045 - SO4266 0.17 +0.5 4,443,168 - SO4266 0.28 -0.4 4,443,182 + SO4266 0.30 +0.3 4,443,189 + -1.9 4,443,189 + SO4266 0.30 +1.0 4,443,190 - SO4266 0.30 +1.2 4,443,190 - SO4266 0.30 -0.9 4,443,190 - SO4266 0.30 +0.4 4,443,190 - SO4266 0.30 +0.6 4,443,285 + SO4266 0.39 -0.3 4,443,312 - SO4266 0.41 -1.0 4,443,350 - SO4266 0.45 +1.0 4,443,355 + SO4266 0.45 -0.0 4,443,374 + SO4266 0.47 +0.6 4,443,383 + SO4266 0.48 +0.6 4,443,383 + SO4266 0.48 +1.0 4,443,552 + SO4266 0.63 +0.3 4,443,575 + SO4266 0.65 -0.1 4,443,584 - SO4266 0.66 +0.1 4,443,584 - SO4266 0.66 +0.9 4,443,644 - -0.1 4,443,691 - SO4266 0.75 +0.0 4,443,766 + SO4266 0.82 -0.2 4,443,774 - SO4266 0.82 +0.4 4,443,781 - SO4266 0.83 +0.5 4,443,898 - +0.5 4,443,931 + +0.4 4,444,024 - -0.4 4,444,127 + +0.2 4,444,127 + -0.5 4,444,135 - -0.6 4,444,135 - +2.0 4,444,197 + -0.1 4,444,217 + +1.0 4,444,217 + -1.1 4,444,225 - -0.8 4,444,232 + -0.1 4,444,232 + +0.1 4,444,242 + +0.5 4,444,251 + +2.8 4,444,296 + +0.5 4,444,341 + -0.0 4,444,349 - +0.3 4,444,351 - -1.0 4,444,391 + +0.6 4,444,443 + -1.0 4,444,494 + -0.4 4,444,502 - -0.3 4,444,502 - +1.8 4,444,510 + +0.1 4,444,518 - -0.2 4,444,518 - -0.3 4,444,528 - +0.2 4,444,622 + -0.8 4,444,630 - +1.1 4,444,657 + hsdR-2 SO4267 0.11 -0.3 4,444,699 - hsdR-2 SO4267 0.12 +0.1 4,444,703 + hsdR-2 SO4267 0.12 -0.2 4,444,711 - hsdR-2 SO4267 0.12 +0.9 4,444,711 - hsdR-2 SO4267 0.12 -0.8 4,444,752 - hsdR-2 SO4267 0.13 -0.1 4,444,822 + hsdR-2 SO4267 0.15 -0.1 4,444,830 - hsdR-2 SO4267 0.16 -0.5 4,444,830 - hsdR-2 SO4267 0.16 -0.9 4,444,837 + hsdR-2 SO4267 0.16 +0.3 4,444,852 - hsdR-2 SO4267 0.16 +1.1 4,444,856 - hsdR-2 SO4267 0.16 -0.8 4,444,890 + hsdR-2 SO4267 0.17 +2.1
Or see this region's nucleotide sequence