Experiment: SBM - with RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sfcA and SO3856 are separated by 73 nucleotides SO3856 and SO3857 are separated by 23 nucleotides SO3857 and SO3858 are separated by 118 nucleotides
SO3855: sfcA - malate oxidoreductase (NCBI ptt file), at 4,000,788 to 4,002,476
sfcA
SO3856: SO3856 - hypothetical protein (NCBI ptt file), at 4,002,550 to 4,002,984
SO3856
SO3857: SO3857 - PAP2 family protein (NCBI ptt file), at 4,003,008 to 4,003,535
SO3857
SO3858: SO3858 - ISSod4, transposase (NCBI ptt file), at 4,003,654 to 4,004,856
SO3858
Position (kb)
4002
4003 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4001.619 kb on - strand, within sfcA at 4001.647 kb on + strand, within sfcA at 4001.655 kb on - strand, within sfcA at 4001.685 kb on - strand, within sfcA at 4001.705 kb on - strand, within sfcA at 4001.812 kb on + strand, within sfcA at 4001.939 kb on - strand, within sfcA at 4001.939 kb on - strand, within sfcA at 4001.953 kb on + strand, within sfcA at 4001.953 kb on + strand, within sfcA at 4001.961 kb on - strand, within sfcA at 4001.996 kb on - strand, within sfcA at 4002.090 kb on + strand, within sfcA at 4002.158 kb on + strand, within sfcA at 4002.176 kb on - strand, within sfcA at 4002.197 kb on - strand, within sfcA at 4002.243 kb on + strand, within sfcA at 4002.294 kb on + strand, within sfcA at 4002.378 kb on + strand at 4002.378 kb on + strand at 4002.574 kb on + strand at 4002.585 kb on - strand at 4002.635 kb on - strand, within SO3856 at 4002.635 kb on - strand, within SO3856 at 4002.635 kb on - strand, within SO3856 at 4002.770 kb on + strand, within SO3856 at 4002.778 kb on - strand, within SO3856 at 4002.808 kb on - strand, within SO3856 at 4002.813 kb on - strand, within SO3856 at 4002.813 kb on - strand, within SO3856 at 4002.863 kb on - strand, within SO3856 at 4002.961 kb on - strand at 4003.099 kb on + strand, within SO3857 at 4003.167 kb on + strand, within SO3857 at 4003.247 kb on + strand, within SO3857 at 4003.256 kb on + strand, within SO3857 at 4003.276 kb on - strand, within SO3857 at 4003.325 kb on + strand, within SO3857 at 4003.333 kb on - strand, within SO3857 at 4003.333 kb on - strand, within SO3857 at 4003.334 kb on - strand, within SO3857 at 4003.350 kb on + strand, within SO3857 at 4003.358 kb on + strand, within SO3857 at 4003.358 kb on - strand, within SO3857 at 4003.368 kb on + strand, within SO3857 at 4003.391 kb on - strand, within SO3857 at 4003.440 kb on - strand, within SO3857 at 4003.550 kb on + strand at 4003.577 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SBM - with RNA protect remove 4,001,619 - sfcA SO3855 0.49 -0.1 4,001,647 + sfcA SO3855 0.51 -0.2 4,001,655 - sfcA SO3855 0.51 -0.7 4,001,685 - sfcA SO3855 0.53 +0.1 4,001,705 - sfcA SO3855 0.54 -0.8 4,001,812 + sfcA SO3855 0.61 -0.3 4,001,939 - sfcA SO3855 0.68 +0.1 4,001,939 - sfcA SO3855 0.68 +0.5 4,001,953 + sfcA SO3855 0.69 -1.3 4,001,953 + sfcA SO3855 0.69 -0.2 4,001,961 - sfcA SO3855 0.69 -0.1 4,001,996 - sfcA SO3855 0.72 -0.1 4,002,090 + sfcA SO3855 0.77 +1.1 4,002,158 + sfcA SO3855 0.81 -1.3 4,002,176 - sfcA SO3855 0.82 +2.7 4,002,197 - sfcA SO3855 0.83 -0.3 4,002,243 + sfcA SO3855 0.86 +0.2 4,002,294 + sfcA SO3855 0.89 +0.0 4,002,378 + -0.4 4,002,378 + +0.6 4,002,574 + -0.0 4,002,585 - -0.2 4,002,635 - SO3856 0.20 +0.5 4,002,635 - SO3856 0.20 -0.5 4,002,635 - SO3856 0.20 -0.4 4,002,770 + SO3856 0.51 -0.7 4,002,778 - SO3856 0.52 -0.9 4,002,808 - SO3856 0.59 -0.2 4,002,813 - SO3856 0.60 -2.1 4,002,813 - SO3856 0.60 +0.4 4,002,863 - SO3856 0.72 -1.2 4,002,961 - +0.5 4,003,099 + SO3857 0.17 +0.3 4,003,167 + SO3857 0.30 +1.5 4,003,247 + SO3857 0.45 -0.1 4,003,256 + SO3857 0.47 +0.9 4,003,276 - SO3857 0.51 -0.0 4,003,325 + SO3857 0.60 -1.9 4,003,333 - SO3857 0.62 +0.2 4,003,333 - SO3857 0.62 +0.0 4,003,334 - SO3857 0.62 +0.5 4,003,350 + SO3857 0.65 -0.9 4,003,358 + SO3857 0.66 +0.2 4,003,358 - SO3857 0.66 -1.7 4,003,368 + SO3857 0.68 -0.5 4,003,391 - SO3857 0.73 +0.6 4,003,440 - SO3857 0.82 +0.3 4,003,550 + +0.8 4,003,577 - +1.1
Or see this region's nucleotide sequence