Experiment: SBM - with RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hmuU and hmuV are separated by 11 nucleotides hmuV and SO3676 are separated by 86 nucleotides SO3676 and SO3677 overlap by 4 nucleotides SO3677 and SO3678 are separated by 99 nucleotides
SO3674: hmuU - hemin ABC transporter, permease protein (NCBI ptt file), at 3,827,012 to 3,828,028
hmuU
SO_3675: hmuV - hemin importer ATP-binding subunit (RefSeq), at 3,828,040 to 3,828,858
hmuV
SO3676: SO3676 - hypothetical protein (NCBI ptt file), at 3,828,945 to 3,829,286
SO3676
SO3677: SO3677 - hypothetical protein (NCBI ptt file), at 3,829,283 to 3,829,651
SO3677
SO3678: SO3678 - conserved hypothetical protein (NCBI ptt file), at 3,829,751 to 3,831,220
SO3678
Position (kb)
3828
3829 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3827.067 kb on + strand at 3827.080 kb on - strand at 3827.134 kb on - strand, within hmuU at 3827.158 kb on - strand, within hmuU at 3827.203 kb on + strand, within hmuU at 3827.243 kb on + strand, within hmuU at 3827.340 kb on + strand, within hmuU at 3827.417 kb on - strand, within hmuU at 3827.462 kb on - strand, within hmuU at 3827.500 kb on + strand, within hmuU at 3827.510 kb on + strand, within hmuU at 3827.510 kb on + strand, within hmuU at 3827.524 kb on + strand, within hmuU at 3827.556 kb on + strand, within hmuU at 3827.556 kb on + strand, within hmuU at 3827.625 kb on + strand, within hmuU at 3827.713 kb on + strand, within hmuU at 3827.744 kb on + strand, within hmuU at 3827.744 kb on + strand, within hmuU at 3827.744 kb on + strand, within hmuU at 3827.752 kb on - strand, within hmuU at 3827.752 kb on - strand, within hmuU at 3827.815 kb on - strand, within hmuU at 3827.976 kb on - strand at 3828.025 kb on - strand at 3828.036 kb on + strand at 3828.043 kb on + strand at 3828.043 kb on + strand at 3828.043 kb on + strand at 3828.114 kb on + strand at 3828.147 kb on - strand, within hmuV at 3828.147 kb on - strand, within hmuV at 3828.147 kb on - strand, within hmuV at 3828.149 kb on + strand, within hmuV at 3828.208 kb on + strand, within hmuV at 3828.215 kb on - strand, within hmuV at 3828.222 kb on + strand, within hmuV at 3828.222 kb on + strand, within hmuV at 3828.222 kb on + strand, within hmuV at 3828.228 kb on - strand, within hmuV at 3828.228 kb on - strand, within hmuV at 3828.230 kb on - strand, within hmuV at 3828.230 kb on - strand, within hmuV at 3828.241 kb on + strand, within hmuV at 3828.261 kb on - strand, within hmuV at 3828.261 kb on - strand, within hmuV at 3828.261 kb on - strand, within hmuV at 3828.263 kb on + strand, within hmuV at 3828.271 kb on - strand, within hmuV at 3828.345 kb on - strand, within hmuV at 3828.347 kb on + strand, within hmuV at 3828.360 kb on - strand, within hmuV at 3828.367 kb on - strand, within hmuV at 3828.489 kb on - strand, within hmuV at 3828.517 kb on - strand, within hmuV at 3828.600 kb on + strand, within hmuV at 3828.622 kb on + strand, within hmuV at 3828.622 kb on + strand, within hmuV at 3828.630 kb on - strand, within hmuV at 3828.630 kb on - strand, within hmuV at 3828.725 kb on + strand, within hmuV at 3828.733 kb on - strand, within hmuV at 3828.733 kb on - strand, within hmuV at 3828.804 kb on - strand at 3828.837 kb on - strand at 3828.890 kb on + strand at 3828.898 kb on - strand at 3828.903 kb on - strand at 3828.903 kb on - strand at 3828.937 kb on - strand at 3829.030 kb on + strand, within SO3676 at 3829.164 kb on + strand, within SO3676 at 3829.245 kb on + strand, within SO3676 at 3829.456 kb on - strand, within SO3677 at 3829.500 kb on + strand, within SO3677 at 3829.526 kb on - strand, within SO3677 at 3829.536 kb on - strand, within SO3677 at 3829.578 kb on + strand, within SO3677 at 3829.654 kb on + strand at 3829.657 kb on + strand at 3829.659 kb on + strand at 3829.834 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SBM - with RNA protect remove 3,827,067 + -0.3 3,827,080 - +0.8 3,827,134 - hmuU SO3674 0.12 -0.6 3,827,158 - hmuU SO3674 0.14 +0.6 3,827,203 + hmuU SO3674 0.19 -0.3 3,827,243 + hmuU SO3674 0.23 -1.6 3,827,340 + hmuU SO3674 0.32 -1.1 3,827,417 - hmuU SO3674 0.40 -1.7 3,827,462 - hmuU SO3674 0.44 +1.3 3,827,500 + hmuU SO3674 0.48 +0.2 3,827,510 + hmuU SO3674 0.49 +0.7 3,827,510 + hmuU SO3674 0.49 -0.1 3,827,524 + hmuU SO3674 0.50 -0.6 3,827,556 + hmuU SO3674 0.53 -1.6 3,827,556 + hmuU SO3674 0.53 -0.2 3,827,625 + hmuU SO3674 0.60 -1.2 3,827,713 + hmuU SO3674 0.69 +1.1 3,827,744 + hmuU SO3674 0.72 +0.1 3,827,744 + hmuU SO3674 0.72 -0.8 3,827,744 + hmuU SO3674 0.72 -1.2 3,827,752 - hmuU SO3674 0.73 -0.1 3,827,752 - hmuU SO3674 0.73 -0.3 3,827,815 - hmuU SO3674 0.79 +0.4 3,827,976 - +0.7 3,828,025 - +0.9 3,828,036 + +1.0 3,828,043 + -0.7 3,828,043 + +0.5 3,828,043 + -1.0 3,828,114 + +0.5 3,828,147 - hmuV SO_3675 0.13 -0.5 3,828,147 - hmuV SO_3675 0.13 +1.4 3,828,147 - hmuV SO_3675 0.13 +0.1 3,828,149 + hmuV SO_3675 0.13 -0.6 3,828,208 + hmuV SO_3675 0.21 +0.1 3,828,215 - hmuV SO_3675 0.21 +1.4 3,828,222 + hmuV SO_3675 0.22 +0.3 3,828,222 + hmuV SO_3675 0.22 -0.3 3,828,222 + hmuV SO_3675 0.22 -0.1 3,828,228 - hmuV SO_3675 0.23 +1.0 3,828,228 - hmuV SO_3675 0.23 -0.0 3,828,230 - hmuV SO_3675 0.23 -0.7 3,828,230 - hmuV SO_3675 0.23 +0.7 3,828,241 + hmuV SO_3675 0.25 +0.6 3,828,261 - hmuV SO_3675 0.27 -1.5 3,828,261 - hmuV SO_3675 0.27 +0.6 3,828,261 - hmuV SO_3675 0.27 +1.4 3,828,263 + hmuV SO_3675 0.27 +0.1 3,828,271 - hmuV SO_3675 0.28 +1.3 3,828,345 - hmuV SO_3675 0.37 +0.3 3,828,347 + hmuV SO_3675 0.37 -0.3 3,828,360 - hmuV SO_3675 0.39 -0.7 3,828,367 - hmuV SO_3675 0.40 +1.2 3,828,489 - hmuV SO_3675 0.55 +0.5 3,828,517 - hmuV SO_3675 0.58 +1.2 3,828,600 + hmuV SO_3675 0.68 +0.3 3,828,622 + hmuV SO_3675 0.71 +0.7 3,828,622 + hmuV SO_3675 0.71 -0.1 3,828,630 - hmuV SO_3675 0.72 -2.0 3,828,630 - hmuV SO_3675 0.72 +0.4 3,828,725 + hmuV SO_3675 0.84 +1.4 3,828,733 - hmuV SO_3675 0.85 -0.3 3,828,733 - hmuV SO_3675 0.85 +0.4 3,828,804 - -1.7 3,828,837 - +0.0 3,828,890 + +0.3 3,828,898 - +0.2 3,828,903 - -1.5 3,828,903 - -0.2 3,828,937 - -0.3 3,829,030 + SO3676 0.25 +4.0 3,829,164 + SO3676 0.64 -0.6 3,829,245 + SO3676 0.88 +0.4 3,829,456 - SO3677 0.47 +0.9 3,829,500 + SO3677 0.59 +1.4 3,829,526 - SO3677 0.66 -0.3 3,829,536 - SO3677 0.69 -0.8 3,829,578 + SO3677 0.80 +0.1 3,829,654 + -0.1 3,829,657 + +0.2 3,829,659 + -0.0 3,829,834 - -0.4
Or see this region's nucleotide sequence