Experiment: SBM - with RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt recN and pgpA are separated by 136 nucleotides pgpA and thiL overlap by 1 nucleotides thiL and nusB are separated by 78 nucleotides nusB and ribH are separated by 14 nucleotides
SO3462: recN - DNA repair protein RecN (NCBI ptt file), at 3,607,978 to 3,609,636
recN
SO3463: pgpA - phosphatidylglycerophosphatase A (NCBI ptt file), at 3,609,773 to 3,610,267
pgpA
SO3464: thiL - thiamin-monophosphate kinase (NCBI ptt file), at 3,610,267 to 3,611,223
thiL
SO3465: nusB - N utilization substance protein B (NCBI ptt file), at 3,611,302 to 3,611,706
nusB
SO3466: ribH - riboflavin synthase, beta subunit (NCBI ptt file), at 3,611,721 to 3,612,197
ribH
Position (kb) 3610
3611
3612 Strain fitness (log2 ratio) -1
0
1 at 3609.396 kb on - strand, within recN at 3609.413 kb on + strand, within recN at 3609.413 kb on + strand, within recN at 3609.413 kb on + strand, within recN at 3609.421 kb on - strand, within recN at 3609.421 kb on - strand, within recN at 3609.998 kb on - strand, within pgpA at 3610.027 kb on - strand, within pgpA
Per-strain Table
Position Strand Gene LocusTag Fraction SBM - with RNA protect remove 3,609,396 - recN SO3462 0.85 +0.4 3,609,413 + recN SO3462 0.86 -0.6 3,609,413 + recN SO3462 0.86 -0.3 3,609,413 + recN SO3462 0.86 +0.9 3,609,421 - recN SO3462 0.87 +0.0 3,609,421 - recN SO3462 0.87 -0.2 3,609,998 - pgpA SO3463 0.45 +1.4 3,610,027 - pgpA SO3463 0.51 +1.4
Or see this region's nucleotide sequence