Strain Fitness in Shewanella oneidensis MR-1 around SO2561

Experiment: SBM - with RNA protect

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdnaQ-2 and rnhA are separated by 70 nucleotidesrnhA and SO2561 are separated by 28 nucleotidesSO2561 and SO2562 are separated by 23 nucleotidesSO2562 and SO2563 are separated by 160 nucleotides SO2559: dnaQ-2 - DNA polymerase III, epsilon subunit (NCBI ptt file), at 2,689,376 to 2,690,104 dnaQ-2 SO2560: rnhA - ribonuclease HI (NCBI ptt file), at 2,690,175 to 2,690,651 rnhA SO2561: SO2561 - transcriptional regulator, LysR family (NCBI ptt file), at 2,690,680 to 2,691,579 SO2561 SO2562: SO2562 - conserved hypothetical protein (NCBI ptt file), at 2,691,603 to 2,692,337 SO2562 SO2563: SO2563 - metallo-beta-lactamase family protein (NCBI ptt file), at 2,692,498 to 2,693,301 SO2563 Position (kb) 2690 2691 2692Strain fitness (log2 ratio) -1 0 1 2at 2690.549 kb on - strand, within rnhAat 2690.730 kb on + strandat 2690.802 kb on - strand, within SO2561at 2690.812 kb on + strand, within SO2561at 2690.820 kb on - strand, within SO2561at 2691.002 kb on + strand, within SO2561at 2691.010 kb on - strand, within SO2561at 2691.130 kb on - strand, within SO2561at 2691.389 kb on - strand, within SO2561at 2691.485 kb on + strand, within SO2561at 2691.512 kb on + strandat 2691.528 kb on - strandat 2691.530 kb on - strandat 2691.641 kb on - strandat 2691.761 kb on + strand, within SO2562at 2691.769 kb on - strand, within SO2562at 2691.800 kb on - strand, within SO2562at 2691.850 kb on - strand, within SO2562at 2691.852 kb on + strand, within SO2562at 2691.857 kb on + strand, within SO2562at 2691.857 kb on + strand, within SO2562at 2691.860 kb on - strand, within SO2562at 2691.909 kb on - strand, within SO2562at 2691.909 kb on - strand, within SO2562at 2691.954 kb on + strand, within SO2562at 2692.312 kb on - strandat 2692.319 kb on + strandat 2692.321 kb on + strandat 2692.329 kb on - strandat 2692.408 kb on - strandat 2692.501 kb on - strandat 2692.579 kb on - strand, within SO2563

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Per-strain Table

Position Strand Gene LocusTag Fraction SBM - with RNA protect
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2,690,549 - rnhA SO2560 0.78 -1.1
2,690,730 + +0.8
2,690,802 - SO2561 0.14 +0.1
2,690,812 + SO2561 0.15 +0.6
2,690,820 - SO2561 0.16 +0.6
2,691,002 + SO2561 0.36 +0.0
2,691,010 - SO2561 0.37 +0.3
2,691,130 - SO2561 0.50 +1.7
2,691,389 - SO2561 0.79 +0.3
2,691,485 + SO2561 0.89 +0.3
2,691,512 + +1.2
2,691,528 - +2.2
2,691,530 - -1.4
2,691,641 - +0.8
2,691,761 + SO2562 0.21 +0.7
2,691,769 - SO2562 0.23 +0.2
2,691,800 - SO2562 0.27 +1.8
2,691,850 - SO2562 0.34 +0.3
2,691,852 + SO2562 0.34 +0.5
2,691,857 + SO2562 0.35 +0.5
2,691,857 + SO2562 0.35 +0.5
2,691,860 - SO2562 0.35 +0.1
2,691,909 - SO2562 0.42 +0.5
2,691,909 - SO2562 0.42 +1.0
2,691,954 + SO2562 0.48 +0.5
2,692,312 - +0.9
2,692,319 + -0.8
2,692,321 + +0.1
2,692,329 - +1.5
2,692,408 - -0.1
2,692,501 - -0.6
2,692,579 - SO2563 0.10 +0.2

Or see this region's nucleotide sequence