Experiment: SBM - with RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dnaQ-2 and rnhA are separated by 70 nucleotides rnhA and SO2561 are separated by 28 nucleotides SO2561 and SO2562 are separated by 23 nucleotides SO2562 and SO2563 are separated by 160 nucleotides
SO2559: dnaQ-2 - DNA polymerase III, epsilon subunit (NCBI ptt file), at 2,689,376 to 2,690,104
dnaQ-2
SO2560: rnhA - ribonuclease HI (NCBI ptt file), at 2,690,175 to 2,690,651
rnhA
SO2561: SO2561 - transcriptional regulator, LysR family (NCBI ptt file), at 2,690,680 to 2,691,579
SO2561
SO2562: SO2562 - conserved hypothetical protein (NCBI ptt file), at 2,691,603 to 2,692,337
SO2562
SO2563: SO2563 - metallo-beta-lactamase family protein (NCBI ptt file), at 2,692,498 to 2,693,301
SO2563
Position (kb)
2690
2691
2692 Strain fitness (log2 ratio)
-1
0
1
2 at 2690.549 kb on - strand, within rnhA at 2690.730 kb on + strand at 2690.802 kb on - strand, within SO2561 at 2690.812 kb on + strand, within SO2561 at 2690.820 kb on - strand, within SO2561 at 2691.002 kb on + strand, within SO2561 at 2691.010 kb on - strand, within SO2561 at 2691.130 kb on - strand, within SO2561 at 2691.389 kb on - strand, within SO2561 at 2691.485 kb on + strand, within SO2561 at 2691.512 kb on + strand at 2691.528 kb on - strand at 2691.530 kb on - strand at 2691.641 kb on - strand at 2691.761 kb on + strand, within SO2562 at 2691.769 kb on - strand, within SO2562 at 2691.800 kb on - strand, within SO2562 at 2691.850 kb on - strand, within SO2562 at 2691.852 kb on + strand, within SO2562 at 2691.857 kb on + strand, within SO2562 at 2691.857 kb on + strand, within SO2562 at 2691.860 kb on - strand, within SO2562 at 2691.909 kb on - strand, within SO2562 at 2691.909 kb on - strand, within SO2562 at 2691.954 kb on + strand, within SO2562 at 2692.312 kb on - strand at 2692.319 kb on + strand at 2692.321 kb on + strand at 2692.329 kb on - strand at 2692.408 kb on - strand at 2692.501 kb on - strand at 2692.579 kb on - strand, within SO2563
Per-strain Table
Position Strand Gene LocusTag Fraction SBM - with RNA protect remove 2,690,549 - rnhA SO2560 0.78 -1.1 2,690,730 + +0.8 2,690,802 - SO2561 0.14 +0.1 2,690,812 + SO2561 0.15 +0.6 2,690,820 - SO2561 0.16 +0.6 2,691,002 + SO2561 0.36 +0.0 2,691,010 - SO2561 0.37 +0.3 2,691,130 - SO2561 0.50 +1.7 2,691,389 - SO2561 0.79 +0.3 2,691,485 + SO2561 0.89 +0.3 2,691,512 + +1.2 2,691,528 - +2.2 2,691,530 - -1.4 2,691,641 - +0.8 2,691,761 + SO2562 0.21 +0.7 2,691,769 - SO2562 0.23 +0.2 2,691,800 - SO2562 0.27 +1.8 2,691,850 - SO2562 0.34 +0.3 2,691,852 + SO2562 0.34 +0.5 2,691,857 + SO2562 0.35 +0.5 2,691,857 + SO2562 0.35 +0.5 2,691,860 - SO2562 0.35 +0.1 2,691,909 - SO2562 0.42 +0.5 2,691,909 - SO2562 0.42 +1.0 2,691,954 + SO2562 0.48 +0.5 2,692,312 - +0.9 2,692,319 + -0.8 2,692,321 + +0.1 2,692,329 - +1.5 2,692,408 - -0.1 2,692,501 - -0.6 2,692,579 - SO2563 0.10 +0.2
Or see this region's nucleotide sequence