Experiment: SBM - with RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysE and SO2263 are separated by 353 nucleotides SO2263 and iscS are separated by 78 nucleotides iscS and SO2265 are separated by 37 nucleotides SO2265 and SO2266 are separated by 14 nucleotides SO2266 and hscB are separated by 15 nucleotides
SO2262: cysE - serine acetyltransferase (NCBI ptt file), at 2,377,236 to 2,378,057
cysE
SO2263: SO2263 - Rrf2 family protein (NCBI ptt file), at 2,378,411 to 2,378,872
SO2263
SO2264: iscS - cysteine desulfurase (NCBI ptt file), at 2,378,951 to 2,380,165
iscS
SO2265: SO2265 - NifU family protein (NCBI ptt file), at 2,380,203 to 2,380,586
SO2265
SO2266: SO2266 - HesB/YadR/YfhF family protein (NCBI ptt file), at 2,380,601 to 2,380,924
SO2266
SO2267: hscB - co-chaperone Hsc20 (NCBI ptt file), at 2,380,940 to 2,381,464
hscB
Position (kb)
2378
2379
2380
2381 Strain fitness (log2 ratio)
-1
0
1 at 2378.046 kb on - strand at 2378.168 kb on - strand at 2378.172 kb on + strand at 2378.316 kb on + strand at 2378.657 kb on + strand, within SO2263 at 2378.657 kb on + strand, within SO2263 at 2378.657 kb on + strand, within SO2263 at 2378.843 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SBM - with RNA protect remove 2,378,046 - +1.1 2,378,168 - +0.2 2,378,172 + +1.7 2,378,316 + -0.7 2,378,657 + SO2263 0.53 +0.5 2,378,657 + SO2263 0.53 -0.9 2,378,657 + SO2263 0.53 -1.2 2,378,843 + -0.1
Or see this region's nucleotide sequence