Strain Fitness in Shewanella oneidensis MR-1 around SO2264

Experiment: SBM - with RNA protect

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcysE and SO2263 are separated by 353 nucleotidesSO2263 and iscS are separated by 78 nucleotidesiscS and SO2265 are separated by 37 nucleotidesSO2265 and SO2266 are separated by 14 nucleotidesSO2266 and hscB are separated by 15 nucleotides SO2262: cysE - serine acetyltransferase (NCBI ptt file), at 2,377,236 to 2,378,057 cysE SO2263: SO2263 - Rrf2 family protein (NCBI ptt file), at 2,378,411 to 2,378,872 SO2263 SO2264: iscS - cysteine desulfurase (NCBI ptt file), at 2,378,951 to 2,380,165 iscS SO2265: SO2265 - NifU family protein (NCBI ptt file), at 2,380,203 to 2,380,586 SO2265 SO2266: SO2266 - HesB/YadR/YfhF family protein (NCBI ptt file), at 2,380,601 to 2,380,924 SO2266 SO2267: hscB - co-chaperone Hsc20 (NCBI ptt file), at 2,380,940 to 2,381,464 hscB Position (kb) 2378 2379 2380 2381Strain fitness (log2 ratio) -1 0 1at 2378.046 kb on - strandat 2378.168 kb on - strandat 2378.172 kb on + strandat 2378.316 kb on + strandat 2378.657 kb on + strand, within SO2263at 2378.657 kb on + strand, within SO2263at 2378.657 kb on + strand, within SO2263at 2378.843 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SBM - with RNA protect
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2,378,046 - +1.1
2,378,168 - +0.2
2,378,172 + +1.7
2,378,316 + -0.7
2,378,657 + SO2263 0.53 +0.5
2,378,657 + SO2263 0.53 -0.9
2,378,657 + SO2263 0.53 -1.2
2,378,843 + -0.1

Or see this region's nucleotide sequence