Experiment: SBM - with RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ivd and SO1898 are separated by 144 nucleotides SO1898 and SO1899 are separated by 260 nucleotides SO1899 and SO_1900 are separated by 169 nucleotides
SO1897: ivd - isovaleryl-CoA dehydrogenase (NCBI ptt file), at 1,995,149 to 1,996,318
ivd
SO1898: SO1898 - transcriptional regulator, putative (NCBI ptt file), at 1,996,463 to 1,996,867
SO1898
SO1899: SO1899 - conserved hypothetical protein (NCBI ptt file), at 1,997,128 to 1,998,843
SO1899
SO_1900: SO_1900 - no description, at 1,999,013 to 2,000,932
_1900
Position (kb)
1997
1998
1999 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1996.264 kb on + strand at 1996.299 kb on + strand at 1996.309 kb on - strand at 1996.534 kb on - strand, within SO1898 at 1996.599 kb on + strand, within SO1898 at 1996.599 kb on + strand, within SO1898 at 1996.599 kb on + strand, within SO1898 at 1996.599 kb on + strand, within SO1898 at 1996.607 kb on - strand, within SO1898 at 1996.622 kb on - strand, within SO1898 at 1996.622 kb on - strand, within SO1898 at 1996.668 kb on - strand, within SO1898 at 1996.693 kb on + strand, within SO1898 at 1996.693 kb on + strand, within SO1898 at 1996.717 kb on + strand, within SO1898 at 1996.723 kb on - strand, within SO1898 at 1996.774 kb on - strand, within SO1898 at 1996.873 kb on - strand at 1996.873 kb on - strand at 1997.057 kb on + strand at 1997.087 kb on - strand at 1997.087 kb on - strand at 1997.134 kb on - strand at 1997.134 kb on - strand at 1997.134 kb on - strand at 1997.138 kb on + strand at 1997.146 kb on - strand at 1997.146 kb on - strand at 1997.185 kb on + strand at 1997.193 kb on - strand at 1997.193 kb on - strand at 1997.351 kb on - strand, within SO1899 at 1997.400 kb on - strand, within SO1899 at 1997.439 kb on - strand, within SO1899 at 1997.468 kb on + strand, within SO1899 at 1997.468 kb on + strand, within SO1899 at 1997.547 kb on + strand, within SO1899 at 1997.592 kb on - strand, within SO1899 at 1997.844 kb on - strand, within SO1899 at 1997.915 kb on - strand, within SO1899 at 1997.917 kb on + strand, within SO1899 at 1997.917 kb on + strand, within SO1899 at 1997.917 kb on + strand, within SO1899 at 1997.930 kb on - strand, within SO1899 at 1997.942 kb on - strand, within SO1899 at 1997.996 kb on - strand, within SO1899 at 1998.128 kb on - strand, within SO1899 at 1998.171 kb on + strand, within SO1899 at 1998.198 kb on - strand, within SO1899 at 1998.324 kb on + strand, within SO1899 at 1998.324 kb on + strand, within SO1899 at 1998.447 kb on - strand, within SO1899 at 1998.517 kb on - strand, within SO1899 at 1998.527 kb on - strand, within SO1899 at 1998.527 kb on - strand, within SO1899 at 1998.561 kb on - strand, within SO1899 at 1998.598 kb on + strand, within SO1899 at 1998.606 kb on - strand, within SO1899 at 1998.606 kb on - strand, within SO1899 at 1998.633 kb on + strand, within SO1899 at 1998.641 kb on + strand, within SO1899 at 1998.652 kb on - strand, within SO1899 at 1998.662 kb on - strand, within SO1899 at 1998.715 kb on + strand at 1998.721 kb on + strand at 1998.903 kb on + strand at 1998.978 kb on + strand at 1999.005 kb on + strand at 1999.100 kb on + strand at 1999.100 kb on + strand at 1999.108 kb on - strand at 1999.108 kb on - strand at 1999.108 kb on - strand at 1999.108 kb on - strand at 1999.178 kb on - strand at 1999.205 kb on + strand, within SO_1900 at 1999.347 kb on + strand, within SO_1900 at 1999.505 kb on - strand, within SO_1900 at 1999.544 kb on + strand, within SO_1900 at 1999.676 kb on + strand, within SO_1900 at 1999.747 kb on - strand, within SO_1900 at 1999.766 kb on + strand, within SO_1900
Per-strain Table
Position Strand Gene LocusTag Fraction SBM - with RNA protect remove 1,996,264 + +0.2 1,996,299 + -1.3 1,996,309 - -0.2 1,996,534 - SO1898 0.18 -0.8 1,996,599 + SO1898 0.34 -1.1 1,996,599 + SO1898 0.34 -0.7 1,996,599 + SO1898 0.34 -1.4 1,996,599 + SO1898 0.34 -0.7 1,996,607 - SO1898 0.36 -1.2 1,996,622 - SO1898 0.39 -1.2 1,996,622 - SO1898 0.39 -0.7 1,996,668 - SO1898 0.51 -2.3 1,996,693 + SO1898 0.57 -0.3 1,996,693 + SO1898 0.57 -1.1 1,996,717 + SO1898 0.63 -2.6 1,996,723 - SO1898 0.64 -2.3 1,996,774 - SO1898 0.77 -1.5 1,996,873 - -0.6 1,996,873 - -0.4 1,997,057 + +0.1 1,997,087 - +0.2 1,997,087 - -0.3 1,997,134 - +0.5 1,997,134 - -0.5 1,997,134 - +0.4 1,997,138 + -0.4 1,997,146 - +0.4 1,997,146 - +1.3 1,997,185 + +0.8 1,997,193 - -0.2 1,997,193 - +0.5 1,997,351 - SO1899 0.13 +0.4 1,997,400 - SO1899 0.16 +0.4 1,997,439 - SO1899 0.18 +0.3 1,997,468 + SO1899 0.20 +0.6 1,997,468 + SO1899 0.20 +2.2 1,997,547 + SO1899 0.24 +0.3 1,997,592 - SO1899 0.27 +2.1 1,997,844 - SO1899 0.42 -0.1 1,997,915 - SO1899 0.46 +0.7 1,997,917 + SO1899 0.46 +0.3 1,997,917 + SO1899 0.46 +1.4 1,997,917 + SO1899 0.46 +0.1 1,997,930 - SO1899 0.47 +1.3 1,997,942 - SO1899 0.47 +1.3 1,997,996 - SO1899 0.51 +0.1 1,998,128 - SO1899 0.58 +1.2 1,998,171 + SO1899 0.61 +0.6 1,998,198 - SO1899 0.62 +0.2 1,998,324 + SO1899 0.70 +1.0 1,998,324 + SO1899 0.70 +0.3 1,998,447 - SO1899 0.77 +0.9 1,998,517 - SO1899 0.81 +1.2 1,998,527 - SO1899 0.82 +0.2 1,998,527 - SO1899 0.82 +1.0 1,998,561 - SO1899 0.84 +0.4 1,998,598 + SO1899 0.86 +0.4 1,998,606 - SO1899 0.86 +0.1 1,998,606 - SO1899 0.86 +1.0 1,998,633 + SO1899 0.88 -0.0 1,998,641 + SO1899 0.88 +1.5 1,998,652 - SO1899 0.89 +1.0 1,998,662 - SO1899 0.89 -0.7 1,998,715 + -0.2 1,998,721 + +1.3 1,998,903 + +0.6 1,998,978 + -0.3 1,999,005 + -0.4 1,999,100 + +1.3 1,999,100 + +0.1 1,999,108 - -0.0 1,999,108 - -0.6 1,999,108 - -0.6 1,999,108 - +0.7 1,999,178 - -0.1 1,999,205 + SO_1900 0.10 -0.3 1,999,347 + SO_1900 0.17 -1.6 1,999,505 - SO_1900 0.26 +1.1 1,999,544 + SO_1900 0.28 +0.6 1,999,676 + SO_1900 0.35 +2.1 1,999,747 - SO_1900 0.38 +1.3 1,999,766 + SO_1900 0.39 -1.3
Or see this region's nucleotide sequence