Experiment: SBM - with RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1829 and SO1830 are separated by 51 nucleotides SO1830 and SO1831 are separated by 80 nucleotides SO1831 and ppiC-2 are separated by 154 nucleotides ppiC-2 and SO1833 are separated by 118 nucleotides
SO1829: SO1829 - TPR domain protein (NCBI ptt file), at 1,921,209 to 1,922,456
SO1829
SO1830: SO1830 - GGDEF domain protein (NCBI ptt file), at 1,922,508 to 1,923,017
SO1830
SO1831: SO1831 - conserved hypothetical protein (NCBI ptt file), at 1,923,098 to 1,924,927
SO1831
SO1832: ppiC-2 - peptidyl-prolyl cis-trans isomerase C (NCBI ptt file), at 1,925,082 to 1,925,360
ppiC-2
SO1833: SO1833 - ISSod7, transposase (NCBI ptt file), at 1,925,479 to 1,926,801
SO1833
Position (kb)
1923
1924
1925 Strain fitness (log2 ratio)
-2
-1
0
1 at 1922.537 kb on + strand at 1922.545 kb on - strand at 1922.802 kb on + strand, within SO1830 at 1922.802 kb on + strand, within SO1830 at 1922.891 kb on + strand, within SO1830 at 1922.906 kb on + strand, within SO1830 at 1922.911 kb on + strand, within SO1830 at 1922.918 kb on - strand, within SO1830 at 1922.918 kb on - strand, within SO1830 at 1922.940 kb on - strand, within SO1830 at 1923.066 kb on - strand at 1923.076 kb on + strand at 1923.148 kb on + strand at 1923.161 kb on - strand at 1923.161 kb on - strand at 1923.335 kb on - strand, within SO1831 at 1923.388 kb on - strand, within SO1831 at 1923.403 kb on + strand, within SO1831 at 1923.403 kb on + strand, within SO1831 at 1923.403 kb on + strand, within SO1831 at 1923.411 kb on - strand, within SO1831 at 1923.503 kb on + strand, within SO1831 at 1923.504 kb on - strand, within SO1831 at 1923.504 kb on - strand, within SO1831 at 1923.559 kb on + strand, within SO1831 at 1923.567 kb on - strand, within SO1831 at 1923.596 kb on + strand, within SO1831 at 1923.604 kb on - strand, within SO1831 at 1923.604 kb on - strand, within SO1831 at 1923.620 kb on - strand, within SO1831 at 1923.664 kb on - strand, within SO1831 at 1923.726 kb on - strand, within SO1831 at 1923.738 kb on + strand, within SO1831 at 1923.753 kb on - strand, within SO1831 at 1923.825 kb on - strand, within SO1831 at 1923.852 kb on + strand, within SO1831 at 1923.862 kb on + strand, within SO1831 at 1923.917 kb on + strand, within SO1831 at 1923.940 kb on - strand, within SO1831 at 1924.004 kb on - strand, within SO1831 at 1924.232 kb on - strand, within SO1831 at 1924.411 kb on + strand, within SO1831 at 1924.480 kb on + strand, within SO1831 at 1924.488 kb on - strand, within SO1831 at 1924.507 kb on + strand, within SO1831 at 1924.643 kb on + strand, within SO1831 at 1924.828 kb on + strand at 1924.857 kb on + strand at 1924.886 kb on + strand at 1924.886 kb on + strand at 1924.888 kb on + strand at 1924.949 kb on + strand at 1924.967 kb on - strand at 1924.967 kb on - strand at 1925.054 kb on + strand at 1925.245 kb on + strand, within ppiC-2 at 1925.245 kb on + strand, within ppiC-2
Per-strain Table
Position Strand Gene LocusTag Fraction SBM - with RNA protect remove 1,922,537 + -0.4 1,922,545 - +1.4 1,922,802 + SO1830 0.58 +0.2 1,922,802 + SO1830 0.58 +0.5 1,922,891 + SO1830 0.75 -0.3 1,922,906 + SO1830 0.78 +0.3 1,922,911 + SO1830 0.79 +0.1 1,922,918 - SO1830 0.80 -0.2 1,922,918 - SO1830 0.80 +0.7 1,922,940 - SO1830 0.85 -0.7 1,923,066 - +0.2 1,923,076 + -0.3 1,923,148 + -0.7 1,923,161 - -1.0 1,923,161 - -0.1 1,923,335 - SO1831 0.13 -0.7 1,923,388 - SO1831 0.16 -1.5 1,923,403 + SO1831 0.17 -0.1 1,923,403 + SO1831 0.17 +0.0 1,923,403 + SO1831 0.17 -0.3 1,923,411 - SO1831 0.17 -1.9 1,923,503 + SO1831 0.22 -0.5 1,923,504 - SO1831 0.22 -0.6 1,923,504 - SO1831 0.22 +1.5 1,923,559 + SO1831 0.25 +0.1 1,923,567 - SO1831 0.26 -0.4 1,923,596 + SO1831 0.27 -0.9 1,923,604 - SO1831 0.28 -0.0 1,923,604 - SO1831 0.28 -0.1 1,923,620 - SO1831 0.29 -0.5 1,923,664 - SO1831 0.31 -0.8 1,923,726 - SO1831 0.34 -0.4 1,923,738 + SO1831 0.35 -1.4 1,923,753 - SO1831 0.36 +0.3 1,923,825 - SO1831 0.40 +0.4 1,923,852 + SO1831 0.41 -0.0 1,923,862 + SO1831 0.42 -1.5 1,923,917 + SO1831 0.45 -0.3 1,923,940 - SO1831 0.46 -1.7 1,924,004 - SO1831 0.50 +0.6 1,924,232 - SO1831 0.62 -0.6 1,924,411 + SO1831 0.72 -1.3 1,924,480 + SO1831 0.76 -0.5 1,924,488 - SO1831 0.76 +0.0 1,924,507 + SO1831 0.77 +0.7 1,924,643 + SO1831 0.84 -1.6 1,924,828 + -0.5 1,924,857 + +0.2 1,924,886 + +0.0 1,924,886 + -1.5 1,924,888 + +0.2 1,924,949 + +0.4 1,924,967 - -0.4 1,924,967 - -1.1 1,925,054 + -1.5 1,925,245 + ppiC-2 SO1832 0.58 +0.5 1,925,245 + ppiC-2 SO1832 0.58 +0.3
Or see this region's nucleotide sequence