Experiment: SBM - with RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1241 and SO1242 are separated by 215 nucleotides SO1242 and SO1244 are separated by 512 nucleotides
SO1241: SO1241 - hypothetical protein (NCBI ptt file), at 1,292,011 to 1,292,988
SO1241
SO1242: SO1242 - hypothetical protein (NCBI ptt file), at 1,293,204 to 1,293,812
SO1242
SO1244: SO1244 - ISSod3, transposase (NCBI ptt file), at 1,294,325 to 1,295,539
SO1244
Position (kb)
1293
1294 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1293.152 kb on + strand at 1293.152 kb on + strand at 1293.225 kb on + strand at 1293.275 kb on + strand, within SO1242 at 1293.275 kb on + strand, within SO1242 at 1293.450 kb on + strand, within SO1242 at 1293.554 kb on + strand, within SO1242 at 1293.562 kb on - strand, within SO1242 at 1293.600 kb on - strand, within SO1242 at 1293.609 kb on + strand, within SO1242 at 1293.609 kb on + strand, within SO1242 at 1293.609 kb on + strand, within SO1242 at 1293.617 kb on - strand, within SO1242 at 1293.627 kb on - strand, within SO1242 at 1293.656 kb on + strand, within SO1242 at 1293.689 kb on + strand, within SO1242 at 1293.717 kb on - strand, within SO1242 at 1293.738 kb on + strand, within SO1242 at 1293.738 kb on + strand, within SO1242 at 1293.746 kb on - strand, within SO1242 at 1293.807 kb on + strand at 1293.910 kb on - strand at 1293.927 kb on + strand at 1293.935 kb on + strand at 1293.954 kb on + strand at 1293.976 kb on + strand at 1294.017 kb on + strand at 1294.018 kb on - strand at 1294.018 kb on - strand at 1294.043 kb on + strand at 1294.051 kb on - strand at 1294.109 kb on - strand at 1294.141 kb on - strand at 1294.220 kb on - strand at 1294.304 kb on + strand at 1294.320 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SBM - with RNA protect remove 1,293,152 + +1.7 1,293,152 + +0.2 1,293,225 + -1.8 1,293,275 + SO1242 0.12 +0.2 1,293,275 + SO1242 0.12 -0.0 1,293,450 + SO1242 0.40 +1.7 1,293,554 + SO1242 0.57 +0.3 1,293,562 - SO1242 0.59 +0.2 1,293,600 - SO1242 0.65 -0.5 1,293,609 + SO1242 0.67 +0.3 1,293,609 + SO1242 0.67 -0.1 1,293,609 + SO1242 0.67 +0.1 1,293,617 - SO1242 0.68 -0.6 1,293,627 - SO1242 0.69 -0.0 1,293,656 + SO1242 0.74 +0.0 1,293,689 + SO1242 0.80 +0.3 1,293,717 - SO1242 0.84 +1.0 1,293,738 + SO1242 0.88 +0.4 1,293,738 + SO1242 0.88 +1.9 1,293,746 - SO1242 0.89 +0.7 1,293,807 + +0.1 1,293,910 - +0.1 1,293,927 + +0.1 1,293,935 + +0.1 1,293,954 + -0.3 1,293,976 + +0.7 1,294,017 + -1.3 1,294,018 - +0.4 1,294,018 - +0.4 1,294,043 + +0.1 1,294,051 - -0.1 1,294,109 - +0.2 1,294,141 - +0.0 1,294,220 - -0.2 1,294,304 + -0.4 1,294,320 - -0.5
Or see this region's nucleotide sequence