Experiment: SBM - with RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt chaA and SO1002 are separated by 195 nucleotides SO1002 and SO1003 are separated by 163 nucleotides
SO1001: chaA - calcium/proton antiporter (NCBI ptt file), at 1,032,741 to 1,033,838
chaA
SO1002: SO1002 - hypothetical protein (NCBI ptt file), at 1,034,034 to 1,036,346
SO1002
SO1003: SO1003 - hypothetical protein (NCBI ptt file), at 1,036,510 to 1,038,243
SO1003
Position (kb)
1034
1035
1036
1037 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1033.169 kb on + strand, within chaA at 1033.175 kb on + strand, within chaA at 1033.189 kb on - strand, within chaA at 1033.192 kb on - strand, within chaA at 1033.248 kb on - strand, within chaA at 1033.293 kb on - strand, within chaA at 1033.327 kb on + strand, within chaA at 1033.346 kb on - strand, within chaA at 1033.386 kb on - strand, within chaA at 1033.445 kb on + strand, within chaA at 1033.448 kb on - strand, within chaA at 1033.469 kb on + strand, within chaA at 1033.477 kb on - strand, within chaA at 1033.631 kb on + strand, within chaA at 1033.698 kb on + strand, within chaA at 1033.771 kb on + strand at 1033.789 kb on - strand at 1033.795 kb on + strand at 1034.031 kb on + strand at 1034.072 kb on + strand at 1034.072 kb on + strand at 1034.082 kb on + strand at 1034.183 kb on + strand at 1034.191 kb on - strand at 1034.232 kb on - strand at 1034.259 kb on + strand at 1034.321 kb on - strand, within SO1002 at 1034.328 kb on + strand, within SO1002 at 1034.336 kb on - strand, within SO1002 at 1034.336 kb on - strand, within SO1002 at 1034.369 kb on - strand, within SO1002 at 1034.472 kb on - strand, within SO1002 at 1034.472 kb on - strand, within SO1002 at 1034.573 kb on + strand, within SO1002 at 1034.638 kb on + strand, within SO1002 at 1034.638 kb on + strand, within SO1002 at 1034.638 kb on + strand, within SO1002 at 1034.638 kb on + strand, within SO1002 at 1034.638 kb on + strand, within SO1002 at 1034.650 kb on + strand, within SO1002 at 1034.695 kb on + strand, within SO1002 at 1034.787 kb on - strand, within SO1002 at 1034.789 kb on + strand, within SO1002 at 1034.789 kb on + strand, within SO1002 at 1034.789 kb on + strand, within SO1002 at 1034.789 kb on + strand, within SO1002 at 1034.797 kb on - strand, within SO1002 at 1034.797 kb on - strand, within SO1002 at 1034.797 kb on - strand, within SO1002 at 1034.797 kb on - strand, within SO1002 at 1034.804 kb on + strand, within SO1002 at 1034.825 kb on - strand, within SO1002 at 1034.984 kb on - strand, within SO1002 at 1035.022 kb on - strand, within SO1002 at 1035.022 kb on - strand, within SO1002 at 1035.030 kb on - strand, within SO1002 at 1035.030 kb on - strand, within SO1002 at 1035.056 kb on - strand, within SO1002 at 1035.107 kb on + strand, within SO1002 at 1035.107 kb on + strand, within SO1002 at 1035.107 kb on + strand, within SO1002 at 1035.107 kb on + strand, within SO1002 at 1035.107 kb on + strand, within SO1002 at 1035.115 kb on - strand, within SO1002 at 1035.179 kb on + strand, within SO1002 at 1035.179 kb on + strand, within SO1002 at 1035.397 kb on + strand, within SO1002 at 1035.397 kb on + strand, within SO1002 at 1035.580 kb on + strand, within SO1002 at 1035.580 kb on + strand, within SO1002 at 1035.586 kb on + strand, within SO1002 at 1035.595 kb on - strand, within SO1002 at 1035.595 kb on - strand, within SO1002 at 1035.607 kb on + strand, within SO1002 at 1035.640 kb on - strand, within SO1002 at 1035.720 kb on + strand, within SO1002 at 1035.728 kb on - strand, within SO1002 at 1036.151 kb on - strand at 1036.273 kb on + strand at 1036.280 kb on + strand at 1036.290 kb on + strand at 1036.290 kb on + strand at 1036.400 kb on - strand at 1036.443 kb on + strand at 1036.459 kb on - strand at 1036.468 kb on + strand at 1036.468 kb on + strand at 1036.475 kb on + strand at 1036.475 kb on + strand at 1036.476 kb on - strand at 1036.476 kb on - strand at 1036.483 kb on - strand at 1036.483 kb on - strand at 1036.502 kb on + strand at 1036.508 kb on + strand at 1036.516 kb on - strand at 1036.610 kb on - strand at 1036.651 kb on - strand at 1036.651 kb on - strand at 1036.684 kb on + strand, within SO1003 at 1036.686 kb on + strand, within SO1003 at 1036.692 kb on - strand, within SO1003 at 1036.694 kb on - strand, within SO1003 at 1036.701 kb on + strand, within SO1003 at 1036.760 kb on + strand, within SO1003 at 1036.760 kb on + strand, within SO1003 at 1036.934 kb on - strand, within SO1003 at 1036.972 kb on - strand, within SO1003 at 1036.988 kb on - strand, within SO1003 at 1037.071 kb on - strand, within SO1003 at 1037.151 kb on + strand, within SO1003 at 1037.159 kb on - strand, within SO1003 at 1037.242 kb on + strand, within SO1003
Per-strain Table
Position Strand Gene LocusTag Fraction SBM - with RNA protect remove 1,033,169 + chaA SO1001 0.39 -2.4 1,033,175 + chaA SO1001 0.40 +0.7 1,033,189 - chaA SO1001 0.41 +0.2 1,033,192 - chaA SO1001 0.41 +2.0 1,033,248 - chaA SO1001 0.46 +1.4 1,033,293 - chaA SO1001 0.50 +0.4 1,033,327 + chaA SO1001 0.53 +1.1 1,033,346 - chaA SO1001 0.55 -0.3 1,033,386 - chaA SO1001 0.59 -0.4 1,033,445 + chaA SO1001 0.64 -2.4 1,033,448 - chaA SO1001 0.64 +0.1 1,033,469 + chaA SO1001 0.66 +0.5 1,033,477 - chaA SO1001 0.67 -1.6 1,033,631 + chaA SO1001 0.81 +0.1 1,033,698 + chaA SO1001 0.87 -0.9 1,033,771 + -0.1 1,033,789 - -0.5 1,033,795 + -0.2 1,034,031 + +0.1 1,034,072 + +0.7 1,034,072 + -0.3 1,034,082 + +0.7 1,034,183 + -1.1 1,034,191 - +0.2 1,034,232 - -2.1 1,034,259 + -0.2 1,034,321 - SO1002 0.12 +0.6 1,034,328 + SO1002 0.13 +0.6 1,034,336 - SO1002 0.13 -1.0 1,034,336 - SO1002 0.13 -0.8 1,034,369 - SO1002 0.14 +0.2 1,034,472 - SO1002 0.19 +1.1 1,034,472 - SO1002 0.19 +0.3 1,034,573 + SO1002 0.23 +0.1 1,034,638 + SO1002 0.26 -0.0 1,034,638 + SO1002 0.26 +0.8 1,034,638 + SO1002 0.26 -0.1 1,034,638 + SO1002 0.26 +1.1 1,034,638 + SO1002 0.26 -0.0 1,034,650 + SO1002 0.27 +1.0 1,034,695 + SO1002 0.29 +0.1 1,034,787 - SO1002 0.33 +0.5 1,034,789 + SO1002 0.33 -2.1 1,034,789 + SO1002 0.33 +0.8 1,034,789 + SO1002 0.33 -0.2 1,034,789 + SO1002 0.33 +0.2 1,034,797 - SO1002 0.33 +0.6 1,034,797 - SO1002 0.33 +0.9 1,034,797 - SO1002 0.33 -0.8 1,034,797 - SO1002 0.33 -2.0 1,034,804 + SO1002 0.33 +1.0 1,034,825 - SO1002 0.34 +1.5 1,034,984 - SO1002 0.41 +2.4 1,035,022 - SO1002 0.43 -0.3 1,035,022 - SO1002 0.43 +0.1 1,035,030 - SO1002 0.43 +0.8 1,035,030 - SO1002 0.43 +1.3 1,035,056 - SO1002 0.44 +0.7 1,035,107 + SO1002 0.46 -0.3 1,035,107 + SO1002 0.46 -0.1 1,035,107 + SO1002 0.46 +0.6 1,035,107 + SO1002 0.46 +1.4 1,035,107 + SO1002 0.46 +0.8 1,035,115 - SO1002 0.47 +0.8 1,035,179 + SO1002 0.50 +0.4 1,035,179 + SO1002 0.50 +0.0 1,035,397 + SO1002 0.59 -0.0 1,035,397 + SO1002 0.59 +1.0 1,035,580 + SO1002 0.67 +0.7 1,035,580 + SO1002 0.67 +0.6 1,035,586 + SO1002 0.67 +0.1 1,035,595 - SO1002 0.67 -0.4 1,035,595 - SO1002 0.67 -0.2 1,035,607 + SO1002 0.68 +2.2 1,035,640 - SO1002 0.69 +0.4 1,035,720 + SO1002 0.73 -1.0 1,035,728 - SO1002 0.73 +2.2 1,036,151 - +0.3 1,036,273 + +0.4 1,036,280 + -0.0 1,036,290 + +0.0 1,036,290 + +2.4 1,036,400 - -0.9 1,036,443 + +0.9 1,036,459 - -0.3 1,036,468 + +0.4 1,036,468 + -1.3 1,036,475 + +1.0 1,036,475 + -0.3 1,036,476 - +1.4 1,036,476 - +0.2 1,036,483 - +2.3 1,036,483 - +0.3 1,036,502 + -1.7 1,036,508 + -0.6 1,036,516 - +0.8 1,036,610 - -0.2 1,036,651 - +1.0 1,036,651 - -0.0 1,036,684 + SO1003 0.10 -0.1 1,036,686 + SO1003 0.10 +1.0 1,036,692 - SO1003 0.10 +0.1 1,036,694 - SO1003 0.11 +0.3 1,036,701 + SO1003 0.11 +0.1 1,036,760 + SO1003 0.14 -0.8 1,036,760 + SO1003 0.14 -1.2 1,036,934 - SO1003 0.24 -0.1 1,036,972 - SO1003 0.27 +0.0 1,036,988 - SO1003 0.28 +0.7 1,037,071 - SO1003 0.32 -0.4 1,037,151 + SO1003 0.37 -1.4 1,037,159 - SO1003 0.37 +0.5 1,037,242 + SO1003 0.42 +0.5
Or see this region's nucleotide sequence