Experiment: SBM - with RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0474 and SO0476 are separated by 245 nucleotides SO0476 and nrfF are separated by 16 nucleotides nrfF and ccmF-2 overlap by 4 nucleotides
SO0474: SO0474 - conserved hypothetical protein (NCBI ptt file), at 498,200 to 498,778
SO0474
SO0476: SO0476 - thioredoxin, putative (NCBI ptt file), at 499,024 to 499,608
SO0476
SO0477: nrfF - cytochrome c-type biogenesis protein NrfF precursor (NCBI ptt file), at 499,625 to 500,074
nrfF
SO0478: ccmF-2 - cytochrome c-type biogenesis protein CcmF (NCBI ptt file), at 500,071 to 502,128
ccmF-2
Position (kb) 499
500
501 Strain fitness (log2 ratio) -2
-1
0
1
2 at 498.631 kb on + strand, within SO0474 at 498.648 kb on + strand, within SO0474 at 498.757 kb on + strand at 498.844 kb on - strand at 498.944 kb on + strand at 498.944 kb on + strand at 498.944 kb on + strand at 498.952 kb on - strand at 499.021 kb on + strand at 499.029 kb on - strand at 499.064 kb on - strand at 499.160 kb on + strand, within SO0476 at 499.395 kb on + strand, within SO0476 at 499.395 kb on + strand, within SO0476 at 499.499 kb on - strand, within SO0476 at 499.501 kb on + strand, within SO0476 at 499.509 kb on - strand, within SO0476 at 499.540 kb on + strand, within SO0476 at 499.540 kb on + strand, within SO0476 at 499.540 kb on + strand, within SO0476 at 499.545 kb on + strand, within SO0476 at 499.550 kb on + strand at 499.558 kb on - strand at 499.563 kb on + strand at 499.571 kb on + strand at 499.579 kb on - strand at 499.585 kb on + strand at 499.618 kb on - strand at 499.618 kb on - strand at 499.676 kb on + strand, within nrfF at 499.684 kb on - strand, within nrfF at 499.688 kb on + strand, within nrfF at 499.688 kb on + strand, within nrfF at 499.688 kb on + strand, within nrfF at 499.696 kb on - strand, within nrfF at 499.696 kb on - strand, within nrfF at 499.696 kb on - strand, within nrfF at 499.696 kb on - strand, within nrfF at 499.781 kb on + strand, within nrfF at 499.898 kb on - strand, within nrfF at 499.903 kb on + strand, within nrfF at 499.911 kb on - strand, within nrfF at 499.911 kb on - strand, within nrfF at 499.911 kb on - strand, within nrfF at 499.911 kb on - strand, within nrfF at 499.926 kb on - strand, within nrfF at 499.981 kb on - strand, within nrfF at 500.014 kb on + strand, within nrfF at 500.045 kb on + strand at 500.045 kb on + strand at 500.096 kb on - strand at 500.103 kb on + strand at 500.103 kb on + strand at 500.108 kb on + strand at 500.108 kb on + strand at 500.111 kb on - strand at 500.127 kb on + strand at 500.325 kb on - strand, within ccmF-2 at 500.470 kb on + strand, within ccmF-2 at 500.478 kb on - strand, within ccmF-2 at 500.493 kb on - strand, within ccmF-2 at 500.499 kb on - strand, within ccmF-2 at 500.507 kb on + strand, within ccmF-2 at 500.507 kb on + strand, within ccmF-2 at 500.507 kb on + strand, within ccmF-2 at 500.507 kb on + strand, within ccmF-2 at 500.507 kb on + strand, within ccmF-2 at 500.515 kb on - strand, within ccmF-2 at 500.515 kb on - strand, within ccmF-2 at 500.570 kb on + strand, within ccmF-2 at 500.570 kb on + strand, within ccmF-2 at 500.607 kb on + strand, within ccmF-2 at 500.607 kb on + strand, within ccmF-2 at 500.615 kb on - strand, within ccmF-2 at 500.673 kb on + strand, within ccmF-2 at 500.673 kb on + strand, within ccmF-2 at 500.673 kb on + strand, within ccmF-2 at 500.673 kb on + strand, within ccmF-2 at 500.681 kb on - strand, within ccmF-2 at 500.681 kb on - strand, within ccmF-2 at 500.681 kb on - strand, within ccmF-2 at 500.681 kb on - strand, within ccmF-2 at 500.697 kb on + strand, within ccmF-2 at 500.716 kb on + strand, within ccmF-2 at 500.716 kb on + strand, within ccmF-2 at 500.732 kb on + strand, within ccmF-2 at 500.754 kb on - strand, within ccmF-2 at 500.769 kb on + strand, within ccmF-2 at 500.769 kb on + strand, within ccmF-2 at 500.777 kb on - strand, within ccmF-2 at 500.802 kb on + strand, within ccmF-2 at 500.810 kb on - strand, within ccmF-2 at 500.810 kb on - strand, within ccmF-2 at 500.810 kb on - strand, within ccmF-2 at 500.810 kb on - strand, within ccmF-2 at 500.810 kb on - strand, within ccmF-2 at 500.831 kb on + strand, within ccmF-2 at 500.839 kb on - strand, within ccmF-2 at 500.873 kb on + strand, within ccmF-2 at 500.884 kb on - strand, within ccmF-2 at 500.954 kb on + strand, within ccmF-2 at 500.956 kb on + strand, within ccmF-2 at 500.956 kb on + strand, within ccmF-2 at 500.987 kb on + strand, within ccmF-2 at 501.047 kb on - strand, within ccmF-2 at 501.058 kb on + strand, within ccmF-2 at 501.063 kb on + strand, within ccmF-2 at 501.066 kb on - strand, within ccmF-2 at 501.071 kb on - strand, within ccmF-2
Per-strain Table
Position Strand Gene LocusTag Fraction SBM - with RNA protect remove 498,631 + SO0474 0.74 +0.2 498,648 + SO0474 0.77 +0.2 498,757 + +0.8 498,844 - +0.1 498,944 + -1.5 498,944 + -0.1 498,944 + -0.0 498,952 - -1.3 499,021 + -0.1 499,029 - +0.0 499,064 - -0.1 499,160 + SO0476 0.23 +1.1 499,395 + SO0476 0.63 +1.5 499,395 + SO0476 0.63 +0.8 499,499 - SO0476 0.81 -0.4 499,501 + SO0476 0.82 +0.7 499,509 - SO0476 0.83 -0.4 499,540 + SO0476 0.88 -0.0 499,540 + SO0476 0.88 -0.2 499,540 + SO0476 0.88 +0.2 499,545 + SO0476 0.89 -0.1 499,550 + +0.7 499,558 - -0.5 499,563 + -0.3 499,571 + +0.8 499,579 - -0.3 499,585 + -2.6 499,618 - -0.7 499,618 - +0.5 499,676 + nrfF SO0477 0.11 +0.4 499,684 - nrfF SO0477 0.13 -0.4 499,688 + nrfF SO0477 0.14 +1.1 499,688 + nrfF SO0477 0.14 +0.7 499,688 + nrfF SO0477 0.14 +0.9 499,696 - nrfF SO0477 0.16 +0.1 499,696 - nrfF SO0477 0.16 +0.3 499,696 - nrfF SO0477 0.16 +0.5 499,696 - nrfF SO0477 0.16 +1.1 499,781 + nrfF SO0477 0.35 +1.3 499,898 - nrfF SO0477 0.61 -0.1 499,903 + nrfF SO0477 0.62 +0.7 499,911 - nrfF SO0477 0.64 +0.5 499,911 - nrfF SO0477 0.64 +0.4 499,911 - nrfF SO0477 0.64 +0.7 499,911 - nrfF SO0477 0.64 -0.3 499,926 - nrfF SO0477 0.67 -1.5 499,981 - nrfF SO0477 0.79 -0.1 500,014 + nrfF SO0477 0.86 +0.5 500,045 + +0.1 500,045 + +1.1 500,096 - +0.0 500,103 + +0.1 500,103 + -0.1 500,108 + -0.0 500,108 + +0.2 500,111 - +0.5 500,127 + -0.1 500,325 - ccmF-2 SO0478 0.12 -0.5 500,470 + ccmF-2 SO0478 0.19 -0.3 500,478 - ccmF-2 SO0478 0.20 +0.7 500,493 - ccmF-2 SO0478 0.21 -0.4 500,499 - ccmF-2 SO0478 0.21 +0.2 500,507 + ccmF-2 SO0478 0.21 +0.1 500,507 + ccmF-2 SO0478 0.21 +0.7 500,507 + ccmF-2 SO0478 0.21 -0.2 500,507 + ccmF-2 SO0478 0.21 -1.1 500,507 + ccmF-2 SO0478 0.21 +1.6 500,515 - ccmF-2 SO0478 0.22 +0.2 500,515 - ccmF-2 SO0478 0.22 +0.5 500,570 + ccmF-2 SO0478 0.24 -0.0 500,570 + ccmF-2 SO0478 0.24 -0.2 500,607 + ccmF-2 SO0478 0.26 +0.8 500,607 + ccmF-2 SO0478 0.26 -0.3 500,615 - ccmF-2 SO0478 0.26 +0.5 500,673 + ccmF-2 SO0478 0.29 +0.5 500,673 + ccmF-2 SO0478 0.29 +0.6 500,673 + ccmF-2 SO0478 0.29 +0.4 500,673 + ccmF-2 SO0478 0.29 +0.2 500,681 - ccmF-2 SO0478 0.30 +0.6 500,681 - ccmF-2 SO0478 0.30 +0.2 500,681 - ccmF-2 SO0478 0.30 -0.1 500,681 - ccmF-2 SO0478 0.30 +0.6 500,697 + ccmF-2 SO0478 0.30 +0.3 500,716 + ccmF-2 SO0478 0.31 +1.0 500,716 + ccmF-2 SO0478 0.31 +1.5 500,732 + ccmF-2 SO0478 0.32 -1.0 500,754 - ccmF-2 SO0478 0.33 +0.3 500,769 + ccmF-2 SO0478 0.34 -0.3 500,769 + ccmF-2 SO0478 0.34 +0.5 500,777 - ccmF-2 SO0478 0.34 +1.1 500,802 + ccmF-2 SO0478 0.36 -0.1 500,810 - ccmF-2 SO0478 0.36 -0.4 500,810 - ccmF-2 SO0478 0.36 -0.4 500,810 - ccmF-2 SO0478 0.36 +0.3 500,810 - ccmF-2 SO0478 0.36 -0.5 500,810 - ccmF-2 SO0478 0.36 +0.5 500,831 + ccmF-2 SO0478 0.37 +0.7 500,839 - ccmF-2 SO0478 0.37 +0.6 500,873 + ccmF-2 SO0478 0.39 -0.1 500,884 - ccmF-2 SO0478 0.40 +0.0 500,954 + ccmF-2 SO0478 0.43 +0.2 500,956 + ccmF-2 SO0478 0.43 -0.0 500,956 + ccmF-2 SO0478 0.43 -0.0 500,987 + ccmF-2 SO0478 0.45 +0.1 501,047 - ccmF-2 SO0478 0.47 -1.5 501,058 + ccmF-2 SO0478 0.48 +2.1 501,063 + ccmF-2 SO0478 0.48 -1.5 501,066 - ccmF-2 SO0478 0.48 -0.2 501,071 - ccmF-2 SO0478 0.49 +0.5
Or see this region's nucleotide sequence