Experiment: SBM - with RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0466 and SO0466.1 are separated by 596 nucleotides SO0466.1 and uvrD overlap by 35 nucleotides uvrD and ubiA are separated by 134 nucleotides
SO0466: SO0466 - hypothetical protein (NCBI ptt file), at 489,412 to 489,942
SO0466
SO0466.1: SO0466.1 - hypothetical protein (NCBI ptt file), at 490,539 to 490,811
SO0466.1
SO0467: uvrD - DNA helicase II (NCBI ptt file), at 490,777 to 492,945
uvrD
SO0468: ubiA - 4-hydroxybenzoate polyprenyl transferase (NCBI ptt file), at 493,080 to 493,940
ubiA
Position (kb) 490
491
492
493 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 489.899 kb on - strand at 489.932 kb on - strand at 489.935 kb on - strand at 489.937 kb on + strand at 489.937 kb on + strand at 489.937 kb on + strand at 489.937 kb on + strand at 489.937 kb on + strand at 489.937 kb on + strand at 489.937 kb on + strand at 489.937 kb on + strand at 489.937 kb on + strand at 489.937 kb on + strand at 489.945 kb on + strand at 489.945 kb on - strand at 489.945 kb on - strand at 489.945 kb on - strand at 489.945 kb on - strand at 489.945 kb on - strand at 489.945 kb on - strand at 489.945 kb on - strand at 489.945 kb on - strand at 489.945 kb on - strand at 489.945 kb on - strand at 489.945 kb on - strand at 489.947 kb on + strand at 489.947 kb on + strand at 489.947 kb on + strand at 489.947 kb on + strand at 489.955 kb on - strand at 489.955 kb on - strand at 489.955 kb on - strand at 489.956 kb on + strand at 489.958 kb on + strand at 489.962 kb on - strand at 489.966 kb on - strand at 489.966 kb on - strand at 489.974 kb on + strand at 489.980 kb on + strand at 489.988 kb on - strand at 490.012 kb on + strand at 490.012 kb on + strand at 490.018 kb on - strand at 490.019 kb on + strand at 490.020 kb on + strand at 490.020 kb on + strand at 490.020 kb on + strand at 490.020 kb on - strand at 490.020 kb on - strand at 490.026 kb on - strand at 490.027 kb on - strand at 490.027 kb on - strand at 490.027 kb on - strand at 490.027 kb on - strand at 490.027 kb on - strand at 490.028 kb on - strand at 490.029 kb on + strand at 490.037 kb on - strand at 490.045 kb on + strand at 490.046 kb on + strand at 490.073 kb on + strand at 490.074 kb on + strand at 490.089 kb on - strand at 490.110 kb on + strand at 490.113 kb on + strand at 490.113 kb on + strand at 490.116 kb on - strand at 490.120 kb on - strand at 490.121 kb on - strand at 490.141 kb on + strand at 490.170 kb on + strand at 490.170 kb on + strand at 490.178 kb on - strand at 490.178 kb on - strand at 490.188 kb on + strand at 490.188 kb on + strand at 490.188 kb on - strand at 490.190 kb on + strand at 490.194 kb on + strand at 490.196 kb on - strand at 490.198 kb on - strand at 490.252 kb on + strand at 490.260 kb on - strand at 490.299 kb on - strand at 490.299 kb on - strand at 490.302 kb on - strand at 490.309 kb on + strand at 490.396 kb on + strand at 490.396 kb on + strand at 490.432 kb on + strand at 490.439 kb on - strand at 490.477 kb on + strand at 490.479 kb on + strand at 490.479 kb on + strand at 490.479 kb on + strand at 490.479 kb on + strand at 490.479 kb on + strand at 490.479 kb on + strand at 490.479 kb on + strand at 490.479 kb on + strand at 490.479 kb on + strand at 490.479 kb on + strand at 490.479 kb on + strand at 490.479 kb on + strand at 490.479 kb on + strand at 490.479 kb on + strand at 490.479 kb on + strand at 490.479 kb on + strand at 490.479 kb on + strand at 490.479 kb on + strand at 490.479 kb on + strand at 490.479 kb on + strand at 490.479 kb on + strand at 490.479 kb on + strand at 490.479 kb on + strand at 490.479 kb on + strand at 490.479 kb on + strand at 490.479 kb on + strand at 490.479 kb on + strand at 490.485 kb on - strand at 490.487 kb on - strand at 490.487 kb on - strand at 490.487 kb on - strand at 490.487 kb on - strand at 490.487 kb on - strand at 490.487 kb on - strand at 490.487 kb on - strand at 490.487 kb on - strand at 490.487 kb on - strand at 490.487 kb on - strand at 490.487 kb on - strand at 490.487 kb on - strand at 490.487 kb on - strand at 490.487 kb on - strand at 490.487 kb on - strand at 490.487 kb on - strand at 490.487 kb on - strand at 490.487 kb on - strand at 490.487 kb on - strand at 490.505 kb on - strand at 490.505 kb on - strand at 490.507 kb on - strand at 490.509 kb on + strand at 490.509 kb on + strand at 490.509 kb on + strand at 490.509 kb on + strand at 490.509 kb on + strand at 490.509 kb on + strand at 490.509 kb on + strand at 490.509 kb on + strand at 490.509 kb on + strand at 490.509 kb on + strand at 490.509 kb on + strand at 490.509 kb on + strand at 490.509 kb on + strand at 490.512 kb on - strand at 490.517 kb on - strand at 490.517 kb on - strand at 490.517 kb on - strand at 490.517 kb on - strand at 490.517 kb on - strand at 490.517 kb on - strand at 490.517 kb on - strand at 490.517 kb on - strand at 490.517 kb on - strand at 490.517 kb on - strand at 490.517 kb on - strand at 490.517 kb on - strand at 490.517 kb on - strand at 490.544 kb on - strand at 490.567 kb on + strand, within SO0466.1 at 490.585 kb on + strand, within SO0466.1 at 490.585 kb on - strand, within SO0466.1 at 490.589 kb on - strand, within SO0466.1 at 490.592 kb on - strand, within SO0466.1 at 490.592 kb on - strand, within SO0466.1 at 490.592 kb on - strand, within SO0466.1 at 490.592 kb on - strand, within SO0466.1 at 490.592 kb on - strand, within SO0466.1 at 490.601 kb on + strand, within SO0466.1 at 490.651 kb on - strand, within SO0466.1 at 490.688 kb on - strand, within SO0466.1 at 490.728 kb on + strand, within SO0466.1 at 490.743 kb on + strand, within SO0466.1 at 490.929 kb on + strand at 490.932 kb on - strand at 490.937 kb on - strand at 491.332 kb on - strand, within uvrD at 491.369 kb on + strand, within uvrD at 491.370 kb on + strand, within uvrD at 491.379 kb on + strand, within uvrD at 491.736 kb on + strand, within uvrD at 491.895 kb on - strand, within uvrD at 491.911 kb on + strand, within uvrD at 492.049 kb on - strand, within uvrD at 492.140 kb on + strand, within uvrD at 492.329 kb on + strand, within uvrD at 492.337 kb on - strand, within uvrD at 492.348 kb on - strand, within uvrD at 492.409 kb on + strand, within uvrD at 492.409 kb on + strand, within uvrD at 492.409 kb on + strand, within uvrD at 492.409 kb on + strand, within uvrD at 492.409 kb on + strand, within uvrD at 492.417 kb on - strand, within uvrD at 492.497 kb on + strand, within uvrD at 492.580 kb on + strand, within uvrD at 492.580 kb on + strand, within uvrD at 492.595 kb on - strand, within uvrD at 492.731 kb on - strand at 492.731 kb on - strand at 492.800 kb on + strand at 492.929 kb on + strand at 492.947 kb on - strand at 492.947 kb on - strand at 493.005 kb on + strand at 493.012 kb on + strand at 493.934 kb on + strand at 493.943 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SBM - with RNA protect remove 489,899 - -0.1 489,932 - -0.8 489,935 - +0.6 489,937 + +1.2 489,937 + +0.8 489,937 + +0.3 489,937 + +2.3 489,937 + -0.2 489,937 + -0.5 489,937 + -1.2 489,937 + +0.4 489,937 + +0.3 489,937 + +0.2 489,945 + +0.5 489,945 - +0.4 489,945 - +0.6 489,945 - -0.2 489,945 - +0.1 489,945 - -0.7 489,945 - +0.8 489,945 - -0.4 489,945 - +0.1 489,945 - -0.2 489,945 - -0.4 489,945 - -0.1 489,947 + +0.6 489,947 + +0.2 489,947 + -0.3 489,947 + -1.0 489,955 - -0.6 489,955 - -0.0 489,955 - +0.3 489,956 + +0.2 489,958 + -1.7 489,962 - -0.3 489,966 - -0.3 489,966 - -0.1 489,974 + +0.5 489,980 + -0.1 489,988 - +0.6 490,012 + -0.7 490,012 + +0.4 490,018 - -0.2 490,019 + +1.0 490,020 + -0.2 490,020 + +0.7 490,020 + +0.3 490,020 - -0.9 490,020 - -0.6 490,026 - -0.5 490,027 - -0.5 490,027 - -0.4 490,027 - +0.4 490,027 - -0.4 490,027 - -0.7 490,028 - -0.3 490,029 + -0.3 490,037 - +0.9 490,045 + -0.1 490,046 + -0.8 490,073 + +0.1 490,074 + -0.1 490,089 - +0.5 490,110 + -0.4 490,113 + +0.4 490,113 + +1.6 490,116 - -0.8 490,120 - +0.2 490,121 - -0.1 490,141 + +0.5 490,170 + +0.6 490,170 + -0.6 490,178 - -0.6 490,178 - +0.1 490,188 + +0.6 490,188 + +0.0 490,188 - -1.6 490,190 + +0.0 490,194 + -2.3 490,196 - -0.8 490,198 - +0.1 490,252 + +0.5 490,260 - +0.3 490,299 - -0.1 490,299 - +2.7 490,302 - -0.2 490,309 + +0.3 490,396 + -0.5 490,396 + +0.1 490,432 + -0.5 490,439 - -0.0 490,477 + -0.4 490,479 + -0.3 490,479 + -0.2 490,479 + +0.7 490,479 + -0.1 490,479 + -0.1 490,479 + -0.5 490,479 + -0.2 490,479 + -0.1 490,479 + -0.8 490,479 + +0.3 490,479 + -0.2 490,479 + +2.5 490,479 + -1.4 490,479 + +1.5 490,479 + +0.5 490,479 + +0.5 490,479 + -0.2 490,479 + +0.9 490,479 + -0.0 490,479 + +1.5 490,479 + -0.4 490,479 + -0.4 490,479 + +1.4 490,479 + -1.1 490,479 + -0.1 490,479 + +0.4 490,479 + +0.2 490,485 - -0.8 490,487 - -0.4 490,487 - -0.1 490,487 - -0.6 490,487 - +0.0 490,487 - -0.5 490,487 - -3.0 490,487 - -0.5 490,487 - -0.3 490,487 - -0.1 490,487 - -0.3 490,487 - +0.5 490,487 - +1.0 490,487 - -0.4 490,487 - +0.1 490,487 - -1.1 490,487 - -1.1 490,487 - -0.5 490,487 - -1.3 490,487 - -1.6 490,505 - +0.3 490,505 - +0.3 490,507 - -0.1 490,509 + +0.3 490,509 + -0.3 490,509 + -0.5 490,509 + -0.2 490,509 + -0.2 490,509 + +0.2 490,509 + -0.3 490,509 + -0.2 490,509 + -0.9 490,509 + +0.5 490,509 + -0.5 490,509 + +0.5 490,509 + +1.2 490,512 - -0.9 490,517 - -0.2 490,517 - +0.1 490,517 - -0.3 490,517 - +0.3 490,517 - -1.2 490,517 - -0.8 490,517 - +0.2 490,517 - -0.1 490,517 - -0.2 490,517 - -0.4 490,517 - +0.9 490,517 - +1.2 490,517 - -0.3 490,544 - -0.5 490,567 + SO0466.1 0.10 -0.3 490,585 + SO0466.1 0.17 +0.7 490,585 - SO0466.1 0.17 +1.4 490,589 - SO0466.1 0.18 +0.4 490,592 - SO0466.1 0.19 -2.4 490,592 - SO0466.1 0.19 -0.4 490,592 - SO0466.1 0.19 -0.6 490,592 - SO0466.1 0.19 -2.6 490,592 - SO0466.1 0.19 -0.5 490,601 + SO0466.1 0.23 +0.1 490,651 - SO0466.1 0.41 -0.4 490,688 - SO0466.1 0.55 -0.5 490,728 + SO0466.1 0.69 -0.1 490,743 + SO0466.1 0.75 -0.3 490,929 + +1.5 490,932 - -0.5 490,937 - +1.5 491,332 - uvrD SO0467 0.26 +0.2 491,369 + uvrD SO0467 0.27 +1.5 491,370 + uvrD SO0467 0.27 +1.5 491,379 + uvrD SO0467 0.28 +0.5 491,736 + uvrD SO0467 0.44 -0.1 491,895 - uvrD SO0467 0.52 +0.5 491,911 + uvrD SO0467 0.52 -0.1 492,049 - uvrD SO0467 0.59 +0.5 492,140 + uvrD SO0467 0.63 +1.8 492,329 + uvrD SO0467 0.72 +1.5 492,337 - uvrD SO0467 0.72 +0.6 492,348 - uvrD SO0467 0.72 +1.5 492,409 + uvrD SO0467 0.75 -0.8 492,409 + uvrD SO0467 0.75 -0.5 492,409 + uvrD SO0467 0.75 -0.1 492,409 + uvrD SO0467 0.75 -0.7 492,409 + uvrD SO0467 0.75 +0.1 492,417 - uvrD SO0467 0.76 -2.9 492,497 + uvrD SO0467 0.79 -1.3 492,580 + uvrD SO0467 0.83 +0.5 492,580 + uvrD SO0467 0.83 +0.5 492,595 - uvrD SO0467 0.84 +1.5 492,731 - -1.2 492,731 - +0.6 492,800 + +0.8 492,929 + +1.2 492,947 - +1.5 492,947 - -1.4 493,005 + -2.4 493,012 + -0.4 493,934 + +0.1 493,943 + -0.6
Or see this region's nucleotide sequence