Experiment: SBM - with RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0459 and SO0461 are separated by 341 nucleotides SO0461 and SO0462 are separated by 12 nucleotides SO0462 and SO0463 are separated by 47 nucleotides SO0463 and SO0464 are separated by 34 nucleotides
SO0459: SO0459 - conserved hypothetical protein (NCBI ptt file), at 484,359 to 485,840
SO0459
SO0461: SO0461 - hypothetical protein (NCBI ptt file), at 486,182 to 486,544
SO0461
SO0462: SO0462 - hypothetical protein (NCBI ptt file), at 486,557 to 486,982
SO0462
SO0463: SO0463 - conserved hypothetical protein (NCBI ptt file), at 487,030 to 487,908
SO0463
SO0464: SO0464 - MutT/nudix family protein (NCBI ptt file), at 487,943 to 488,350
SO0464
Position (kb) 486
487 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 485.615 kb on + strand, within SO0459 at 485.621 kb on - strand, within SO0459 at 485.640 kb on - strand, within SO0459 at 485.714 kb on + strand at 485.714 kb on + strand at 485.846 kb on + strand at 485.854 kb on - strand at 485.865 kb on + strand at 485.865 kb on + strand at 485.865 kb on + strand at 485.865 kb on + strand at 485.865 kb on + strand at 485.877 kb on + strand at 485.920 kb on + strand at 485.933 kb on + strand at 485.981 kb on - strand at 485.981 kb on - strand at 485.981 kb on - strand at 485.981 kb on - strand at 485.981 kb on - strand at 486.258 kb on + strand, within SO0461 at 486.258 kb on + strand, within SO0461 at 486.266 kb on - strand, within SO0461 at 486.266 kb on - strand, within SO0461 at 486.266 kb on - strand, within SO0461 at 486.365 kb on - strand, within SO0461 at 486.371 kb on + strand, within SO0461 at 486.374 kb on - strand, within SO0461 at 486.374 kb on - strand, within SO0461 at 486.376 kb on + strand, within SO0461 at 486.379 kb on - strand, within SO0461 at 486.379 kb on - strand, within SO0461 at 486.384 kb on - strand, within SO0461 at 486.391 kb on + strand, within SO0461 at 486.391 kb on + strand, within SO0461 at 486.414 kb on - strand, within SO0461 at 486.443 kb on - strand, within SO0461 at 486.502 kb on + strand, within SO0461 at 486.512 kb on + strand at 486.520 kb on - strand at 486.520 kb on - strand at 486.569 kb on + strand at 486.569 kb on + strand at 486.570 kb on + strand at 486.580 kb on + strand at 486.685 kb on - strand, within SO0462 at 486.702 kb on + strand, within SO0462 at 486.726 kb on + strand, within SO0462 at 486.782 kb on + strand, within SO0462 at 486.810 kb on + strand, within SO0462 at 486.918 kb on - strand, within SO0462 at 487.119 kb on + strand, within SO0463 at 487.126 kb on - strand, within SO0463 at 487.354 kb on + strand, within SO0463 at 487.362 kb on - strand, within SO0463 at 487.375 kb on - strand, within SO0463 at 487.420 kb on - strand, within SO0463 at 487.462 kb on + strand, within SO0463 at 487.470 kb on - strand, within SO0463 at 487.561 kb on + strand, within SO0463 at 487.561 kb on + strand, within SO0463 at 487.561 kb on + strand, within SO0463 at 487.569 kb on - strand, within SO0463 at 487.569 kb on - strand, within SO0463 at 487.583 kb on + strand, within SO0463 at 487.803 kb on + strand, within SO0463 at 487.811 kb on + strand, within SO0463 at 487.940 kb on - strand at 487.966 kb on + strand at 487.967 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SBM - with RNA protect remove 485,615 + SO0459 0.85 -2.1 485,621 - SO0459 0.85 +1.3 485,640 - SO0459 0.86 +0.3 485,714 + -0.2 485,714 + -0.5 485,846 + +0.4 485,854 - +0.2 485,865 + -0.2 485,865 + -0.2 485,865 + -0.8 485,865 + -0.0 485,865 + -3.3 485,877 + +0.8 485,920 + +0.2 485,933 + -0.6 485,981 - -1.1 485,981 - +0.1 485,981 - -0.5 485,981 - +0.5 485,981 - +1.6 486,258 + SO0461 0.21 -1.1 486,258 + SO0461 0.21 -0.8 486,266 - SO0461 0.23 -0.3 486,266 - SO0461 0.23 +0.3 486,266 - SO0461 0.23 +0.6 486,365 - SO0461 0.50 +0.2 486,371 + SO0461 0.52 +0.2 486,374 - SO0461 0.53 -1.1 486,374 - SO0461 0.53 -1.0 486,376 + SO0461 0.53 +0.3 486,379 - SO0461 0.54 +0.4 486,379 - SO0461 0.54 +0.7 486,384 - SO0461 0.56 -0.7 486,391 + SO0461 0.58 +1.3 486,391 + SO0461 0.58 +0.9 486,414 - SO0461 0.64 -0.5 486,443 - SO0461 0.72 -0.0 486,502 + SO0461 0.88 -0.2 486,512 + -0.3 486,520 - +0.0 486,520 - -0.5 486,569 + +0.6 486,569 + +0.1 486,570 + +0.0 486,580 + -0.7 486,685 - SO0462 0.30 -0.2 486,702 + SO0462 0.34 -0.5 486,726 + SO0462 0.40 +0.5 486,782 + SO0462 0.53 -1.2 486,810 + SO0462 0.59 -0.8 486,918 - SO0462 0.85 -1.0 487,119 + SO0463 0.10 -0.8 487,126 - SO0463 0.11 +0.1 487,354 + SO0463 0.37 +0.9 487,362 - SO0463 0.38 -0.3 487,375 - SO0463 0.39 -0.8 487,420 - SO0463 0.44 -0.6 487,462 + SO0463 0.49 +0.2 487,470 - SO0463 0.50 +1.0 487,561 + SO0463 0.60 -1.2 487,561 + SO0463 0.60 -0.3 487,561 + SO0463 0.60 -0.9 487,569 - SO0463 0.61 -0.1 487,569 - SO0463 0.61 -0.3 487,583 + SO0463 0.63 +0.0 487,803 + SO0463 0.88 -3.0 487,811 + SO0463 0.89 -0.8 487,940 - -1.0 487,966 + -2.2 487,967 - -0.7
Or see this region's nucleotide sequence