Experiment: SBM - with RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fdnI and fdhE overlap by 1 nucleotides fdhE and selA are separated by 57 nucleotides selA and selB overlap by 4 nucleotides
SO0103: fdnI - formate dehydrogenase, nitrate-inducible, cytochrome b556 subunit (NCBI ptt file), at 112,631 to 113,272
fdnI
SO0104: fdhE - fdhE protein (NCBI ptt file), at 113,272 to 114,180
fdhE
SO0105: selA - L-seryl-tRNA selenium transferase (NCBI ptt file), at 114,238 to 115,659
selA
SO0106: selB - selenocysteine-specific translation elongation factor (NCBI ptt file), at 115,656 to 117,677
selB
Position (kb)
114
115
116 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 113.299 kb on + strand at 113.332 kb on + strand at 113.340 kb on - strand at 113.349 kb on + strand at 113.349 kb on + strand at 113.349 kb on + strand at 113.349 kb on + strand at 113.349 kb on + strand at 113.357 kb on - strand at 113.357 kb on - strand at 113.357 kb on - strand at 113.364 kb on + strand, within fdhE at 113.428 kb on - strand, within fdhE at 113.428 kb on - strand, within fdhE at 113.472 kb on + strand, within fdhE at 113.583 kb on - strand, within fdhE at 113.608 kb on + strand, within fdhE at 113.645 kb on + strand, within fdhE at 113.669 kb on - strand, within fdhE at 113.694 kb on - strand, within fdhE at 113.770 kb on - strand, within fdhE at 113.780 kb on + strand, within fdhE at 113.790 kb on + strand, within fdhE at 113.803 kb on - strand, within fdhE at 113.830 kb on + strand, within fdhE at 113.870 kb on - strand, within fdhE at 113.872 kb on + strand, within fdhE at 113.880 kb on - strand, within fdhE at 113.886 kb on + strand, within fdhE at 113.929 kb on + strand, within fdhE at 113.949 kb on + strand, within fdhE at 113.949 kb on + strand, within fdhE at 113.949 kb on + strand, within fdhE at 113.957 kb on - strand, within fdhE at 113.981 kb on + strand, within fdhE at 114.009 kb on - strand, within fdhE at 114.104 kb on + strand at 114.117 kb on + strand at 114.133 kb on - strand at 114.149 kb on - strand at 114.203 kb on - strand at 114.262 kb on + strand at 114.268 kb on + strand at 114.268 kb on + strand at 114.268 kb on + strand at 114.276 kb on - strand at 114.276 kb on - strand at 114.276 kb on - strand at 114.414 kb on + strand, within selA at 114.422 kb on - strand, within selA at 114.488 kb on - strand, within selA at 114.565 kb on - strand, within selA at 114.584 kb on + strand, within selA at 114.592 kb on - strand, within selA at 114.676 kb on + strand, within selA at 114.846 kb on - strand, within selA at 114.871 kb on - strand, within selA at 114.879 kb on + strand, within selA at 114.884 kb on + strand, within selA at 114.886 kb on - strand, within selA at 114.886 kb on - strand, within selA at 114.886 kb on - strand, within selA at 114.887 kb on - strand, within selA at 114.895 kb on - strand, within selA at 114.897 kb on + strand, within selA at 114.939 kb on - strand, within selA at 114.971 kb on + strand, within selA at 114.980 kb on + strand, within selA at 114.981 kb on + strand, within selA at 114.988 kb on - strand, within selA at 114.990 kb on + strand, within selA at 114.996 kb on + strand, within selA at 115.004 kb on - strand, within selA at 115.023 kb on - strand, within selA at 115.054 kb on + strand, within selA at 115.265 kb on - strand, within selA at 115.281 kb on + strand, within selA at 115.343 kb on - strand, within selA at 115.418 kb on - strand, within selA at 115.475 kb on + strand, within selA at 115.511 kb on + strand, within selA at 115.519 kb on - strand at 115.519 kb on - strand at 115.645 kb on + strand at 115.688 kb on + strand at 115.694 kb on + strand at 115.699 kb on + strand at 115.702 kb on - strand at 115.702 kb on - strand at 115.890 kb on + strand, within selB at 115.896 kb on + strand, within selB at 115.904 kb on - strand, within selB at 115.904 kb on - strand, within selB at 115.970 kb on - strand, within selB at 115.986 kb on - strand, within selB at 115.986 kb on - strand, within selB at 115.986 kb on - strand, within selB at 115.997 kb on + strand, within selB at 116.041 kb on + strand, within selB at 116.041 kb on + strand, within selB at 116.049 kb on - strand, within selB at 116.067 kb on + strand, within selB at 116.075 kb on - strand, within selB at 116.093 kb on - strand, within selB at 116.136 kb on - strand, within selB at 116.136 kb on - strand, within selB at 116.191 kb on + strand, within selB at 116.199 kb on - strand, within selB at 116.199 kb on - strand, within selB at 116.215 kb on + strand, within selB at 116.229 kb on + strand, within selB at 116.229 kb on + strand, within selB at 116.237 kb on - strand, within selB at 116.237 kb on - strand, within selB at 116.281 kb on - strand, within selB at 116.291 kb on + strand, within selB at 116.297 kb on - strand, within selB at 116.360 kb on + strand, within selB at 116.369 kb on - strand, within selB at 116.369 kb on - strand, within selB at 116.391 kb on - strand, within selB at 116.447 kb on + strand, within selB at 116.452 kb on + strand, within selB at 116.455 kb on - strand, within selB at 116.473 kb on - strand, within selB at 116.475 kb on + strand, within selB at 116.507 kb on - strand, within selB at 116.507 kb on - strand, within selB at 116.507 kb on - strand, within selB at 116.507 kb on - strand, within selB at 116.541 kb on + strand, within selB at 116.547 kb on + strand, within selB at 116.591 kb on - strand, within selB
Per-strain Table
Position Strand Gene LocusTag Fraction SBM - with RNA protect remove 113,299 + -0.7 113,332 + -1.7 113,340 - +0.7 113,349 + +0.3 113,349 + +0.0 113,349 + +0.9 113,349 + +1.1 113,349 + +0.6 113,357 - +0.9 113,357 - +2.0 113,357 - +0.1 113,364 + fdhE SO0104 0.10 +0.9 113,428 - fdhE SO0104 0.17 +1.2 113,428 - fdhE SO0104 0.17 +0.1 113,472 + fdhE SO0104 0.22 +0.2 113,583 - fdhE SO0104 0.34 -0.9 113,608 + fdhE SO0104 0.37 +0.6 113,645 + fdhE SO0104 0.41 -0.3 113,669 - fdhE SO0104 0.44 +1.5 113,694 - fdhE SO0104 0.46 +0.4 113,770 - fdhE SO0104 0.55 +0.1 113,780 + fdhE SO0104 0.56 +0.2 113,790 + fdhE SO0104 0.57 +0.5 113,803 - fdhE SO0104 0.58 +0.0 113,830 + fdhE SO0104 0.61 -0.1 113,870 - fdhE SO0104 0.66 +0.5 113,872 + fdhE SO0104 0.66 +0.4 113,880 - fdhE SO0104 0.67 +0.2 113,886 + fdhE SO0104 0.68 +0.7 113,929 + fdhE SO0104 0.72 +1.5 113,949 + fdhE SO0104 0.74 -1.2 113,949 + fdhE SO0104 0.74 +0.4 113,949 + fdhE SO0104 0.74 -0.4 113,957 - fdhE SO0104 0.75 -0.3 113,981 + fdhE SO0104 0.78 +0.7 114,009 - fdhE SO0104 0.81 -0.7 114,104 + -1.6 114,117 + -0.5 114,133 - +1.0 114,149 - -0.1 114,203 - +0.5 114,262 + +0.6 114,268 + +1.5 114,268 + +2.2 114,268 + +0.4 114,276 - -0.2 114,276 - -0.1 114,276 - +0.2 114,414 + selA SO0105 0.12 -0.1 114,422 - selA SO0105 0.13 +0.6 114,488 - selA SO0105 0.18 +0.7 114,565 - selA SO0105 0.23 +0.4 114,584 + selA SO0105 0.24 +0.4 114,592 - selA SO0105 0.25 +0.2 114,676 + selA SO0105 0.31 +0.3 114,846 - selA SO0105 0.43 +0.2 114,871 - selA SO0105 0.45 +1.4 114,879 + selA SO0105 0.45 +0.2 114,884 + selA SO0105 0.45 +1.7 114,886 - selA SO0105 0.46 -1.0 114,886 - selA SO0105 0.46 +1.2 114,886 - selA SO0105 0.46 -0.4 114,887 - selA SO0105 0.46 +1.5 114,895 - selA SO0105 0.46 -0.0 114,897 + selA SO0105 0.46 +0.1 114,939 - selA SO0105 0.49 -0.1 114,971 + selA SO0105 0.52 +0.2 114,980 + selA SO0105 0.52 +2.2 114,981 + selA SO0105 0.52 +0.4 114,988 - selA SO0105 0.53 +0.6 114,990 + selA SO0105 0.53 -0.3 114,996 + selA SO0105 0.53 +1.1 115,004 - selA SO0105 0.54 +0.8 115,023 - selA SO0105 0.55 +1.2 115,054 + selA SO0105 0.57 +1.3 115,265 - selA SO0105 0.72 -0.9 115,281 + selA SO0105 0.73 +0.2 115,343 - selA SO0105 0.78 -3.4 115,418 - selA SO0105 0.83 +0.3 115,475 + selA SO0105 0.87 +0.1 115,511 + selA SO0105 0.90 -0.3 115,519 - -0.4 115,519 - -0.1 115,645 + +0.5 115,688 + +0.4 115,694 + +0.5 115,699 + +0.2 115,702 - +0.6 115,702 - +0.1 115,890 + selB SO0106 0.12 +1.4 115,896 + selB SO0106 0.12 -0.1 115,904 - selB SO0106 0.12 -0.6 115,904 - selB SO0106 0.12 -1.3 115,970 - selB SO0106 0.16 +0.0 115,986 - selB SO0106 0.16 -0.4 115,986 - selB SO0106 0.16 +0.2 115,986 - selB SO0106 0.16 -0.5 115,997 + selB SO0106 0.17 +1.4 116,041 + selB SO0106 0.19 -0.2 116,041 + selB SO0106 0.19 +0.9 116,049 - selB SO0106 0.19 -0.1 116,067 + selB SO0106 0.20 -0.0 116,075 - selB SO0106 0.21 -0.8 116,093 - selB SO0106 0.22 +0.1 116,136 - selB SO0106 0.24 +0.3 116,136 - selB SO0106 0.24 +0.3 116,191 + selB SO0106 0.26 +0.7 116,199 - selB SO0106 0.27 -0.3 116,199 - selB SO0106 0.27 -0.2 116,215 + selB SO0106 0.28 +0.5 116,229 + selB SO0106 0.28 -0.2 116,229 + selB SO0106 0.28 -0.2 116,237 - selB SO0106 0.29 +0.4 116,237 - selB SO0106 0.29 +0.3 116,281 - selB SO0106 0.31 +0.4 116,291 + selB SO0106 0.31 +0.3 116,297 - selB SO0106 0.32 +1.5 116,360 + selB SO0106 0.35 -0.9 116,369 - selB SO0106 0.35 -0.0 116,369 - selB SO0106 0.35 -0.1 116,391 - selB SO0106 0.36 +1.1 116,447 + selB SO0106 0.39 -2.6 116,452 + selB SO0106 0.39 +0.5 116,455 - selB SO0106 0.40 +0.4 116,473 - selB SO0106 0.40 +1.1 116,475 + selB SO0106 0.41 +0.2 116,507 - selB SO0106 0.42 -0.2 116,507 - selB SO0106 0.42 -0.5 116,507 - selB SO0106 0.42 -1.3 116,507 - selB SO0106 0.42 -0.3 116,541 + selB SO0106 0.44 +0.3 116,547 + selB SO0106 0.44 -0.3 116,591 - selB SO0106 0.46 -0.1
Or see this region's nucleotide sequence