Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20375 and BWI76_RS20380 are separated by 27 nucleotides BWI76_RS20380 and BWI76_RS20385 overlap by 1 nucleotides BWI76_RS20385 and BWI76_RS20390 are separated by 62 nucleotides
BWI76_RS20375: BWI76_RS20375 - membrane protein, at 4,265,207 to 4,265,866
_RS20375
BWI76_RS20380: BWI76_RS20380 - tRNA pseudouridine(38-40) synthase TruA, at 4,265,894 to 4,266,706
_RS20380
BWI76_RS20385: BWI76_RS20385 - aspartate-semialdehyde dehydrogenase, at 4,266,706 to 4,267,719
_RS20385
BWI76_RS20390: BWI76_RS20390 - erythronate-4-phosphate dehydrogenase, at 4,267,782 to 4,268,918
_RS20390
Position (kb) 4266
4267
4268 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4265.756 kb on - strand, within BWI76_RS20375 at 4265.878 kb on + strand at 4265.909 kb on - strand at 4266.308 kb on + strand, within BWI76_RS20380 at 4266.788 kb on + strand at 4266.793 kb on + strand at 4266.921 kb on + strand, within BWI76_RS20385 at 4266.974 kb on - strand, within BWI76_RS20385 at 4267.035 kb on - strand, within BWI76_RS20385 at 4267.590 kb on - strand, within BWI76_RS20385 at 4267.854 kb on - strand at 4268.307 kb on + strand, within BWI76_RS20390 at 4268.380 kb on + strand, within BWI76_RS20390 at 4268.541 kb on - strand, within BWI76_RS20390
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 4,265,756 - BWI76_RS20375 0.83 +1.8 4,265,878 + -0.1 4,265,909 - +0.0 4,266,308 + BWI76_RS20380 0.51 -0.7 4,266,788 + -1.2 4,266,793 + -0.4 4,266,921 + BWI76_RS20385 0.21 -1.1 4,266,974 - BWI76_RS20385 0.26 -2.3 4,267,035 - BWI76_RS20385 0.32 -1.1 4,267,590 - BWI76_RS20385 0.87 -0.1 4,267,854 - +0.9 4,268,307 + BWI76_RS20390 0.46 -0.8 4,268,380 + BWI76_RS20390 0.53 -0.3 4,268,541 - BWI76_RS20390 0.67 -0.9
Or see this region's nucleotide sequence