Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS18945

Experiment: RCH2_defined_glucose with MS_media 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS18935 and BWI76_RS18940 overlap by 1 nucleotidesBWI76_RS18940 and BWI76_RS18945 overlap by 1 nucleotidesBWI76_RS18945 and BWI76_RS18950 overlap by 19 nucleotidesBWI76_RS18950 and BWI76_RS18955 overlap by 7 nucleotides BWI76_RS18935: BWI76_RS18935 - bifunctional imidazole glycerol-phosphate dehydratase/histidinol phosphatase, at 3,927,897 to 3,928,964 _RS18935 BWI76_RS18940: BWI76_RS18940 - imidazole glycerol phosphate synthase subunit HisH, at 3,928,964 to 3,929,554 _RS18940 BWI76_RS18945: BWI76_RS18945 - 1-(5-phosphoribosyl)-5-((5- phosphoribosylamino)methylideneamino)imidazole-4- carboxamide isomerase, at 3,929,554 to 3,930,291 _RS18945 BWI76_RS18950: BWI76_RS18950 - imidazole glycerol phosphate synthase cyclase subunit, at 3,930,273 to 3,931,049 _RS18950 BWI76_RS18955: BWI76_RS18955 - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase, at 3,931,043 to 3,931,642 _RS18955 Position (kb) 3929 3930 3931Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 3928.788 kb on + strand, within BWI76_RS18935at 3928.813 kb on + strand, within BWI76_RS18935at 3928.820 kb on - strand, within BWI76_RS18935at 3928.839 kb on - strand, within BWI76_RS18935at 3929.066 kb on + strand, within BWI76_RS18940at 3929.157 kb on - strand, within BWI76_RS18940at 3929.368 kb on - strand, within BWI76_RS18940at 3929.441 kb on + strand, within BWI76_RS18940at 3929.476 kb on + strand, within BWI76_RS18940at 3930.057 kb on + strand, within BWI76_RS18945at 3930.223 kb on - strandat 3930.377 kb on + strand, within BWI76_RS18950at 3930.426 kb on + strand, within BWI76_RS18950at 3930.445 kb on + strand, within BWI76_RS18950at 3930.909 kb on + strand, within BWI76_RS18950at 3931.121 kb on + strand, within BWI76_RS18955

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x
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3,928,788 + BWI76_RS18935 0.83 -6.4
3,928,813 + BWI76_RS18935 0.86 -5.1
3,928,820 - BWI76_RS18935 0.86 -2.1
3,928,839 - BWI76_RS18935 0.88 -5.3
3,929,066 + BWI76_RS18940 0.17 -2.9
3,929,157 - BWI76_RS18940 0.33 -3.7
3,929,368 - BWI76_RS18940 0.68 -3.9
3,929,441 + BWI76_RS18940 0.81 -4.4
3,929,476 + BWI76_RS18940 0.87 -3.1
3,930,057 + BWI76_RS18945 0.68 -1.6
3,930,223 - -3.2
3,930,377 + BWI76_RS18950 0.13 -6.0
3,930,426 + BWI76_RS18950 0.20 -3.9
3,930,445 + BWI76_RS18950 0.22 -5.2
3,930,909 + BWI76_RS18950 0.82 -3.6
3,931,121 + BWI76_RS18955 0.13 -5.6

Or see this region's nucleotide sequence