Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16790 and BWI76_RS16795 are separated by 100 nucleotides BWI76_RS16795 and BWI76_RS16800 overlap by 14 nucleotides BWI76_RS16800 and BWI76_RS16805 are separated by 65 nucleotides
BWI76_RS16790: BWI76_RS16790 - MFS transporter, at 3,481,340 to 3,482,545
_RS16790
BWI76_RS16795: BWI76_RS16795 - amino acid ABC transporter ATPase, at 3,482,646 to 3,483,368
_RS16795
BWI76_RS16800: BWI76_RS16800 - amino acid ABC transporter permease, at 3,483,355 to 3,484,119
_RS16800
BWI76_RS16805: BWI76_RS16805 - basic amino acid ABC transporter substrate-binding protein, at 3,484,185 to 3,484,952
_RS16805
Position (kb)
3482
3483
3484 Strain fitness (log2 ratio)
-1
0
1 at 3481.739 kb on + strand, within BWI76_RS16790 at 3482.057 kb on - strand, within BWI76_RS16790 at 3482.057 kb on - strand, within BWI76_RS16790 at 3482.144 kb on + strand, within BWI76_RS16790 at 3482.144 kb on + strand, within BWI76_RS16790 at 3482.144 kb on + strand, within BWI76_RS16790 at 3482.258 kb on + strand, within BWI76_RS16790 at 3482.537 kb on + strand at 3482.553 kb on - strand at 3482.718 kb on - strand at 3482.878 kb on - strand, within BWI76_RS16795 at 3482.894 kb on + strand, within BWI76_RS16795 at 3483.505 kb on - strand, within BWI76_RS16800 at 3484.146 kb on + strand at 3484.161 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 3,481,739 + BWI76_RS16790 0.33 +0.2 3,482,057 - BWI76_RS16790 0.59 -0.2 3,482,057 - BWI76_RS16790 0.59 -1.3 3,482,144 + BWI76_RS16790 0.67 -0.2 3,482,144 + BWI76_RS16790 0.67 +0.5 3,482,144 + BWI76_RS16790 0.67 -0.5 3,482,258 + BWI76_RS16790 0.76 -0.4 3,482,537 + +0.1 3,482,553 - -0.1 3,482,718 - -0.3 3,482,878 - BWI76_RS16795 0.32 +0.7 3,482,894 + BWI76_RS16795 0.34 +0.1 3,483,505 - BWI76_RS16800 0.20 -0.1 3,484,146 + -0.0 3,484,161 + -1.7
Or see this region's nucleotide sequence