Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS15745

Experiment: RCH2_defined_glucose with MS_media 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS15730 and BWI76_RS15735 overlap by 8 nucleotidesBWI76_RS15735 and BWI76_RS15740 overlap by 4 nucleotidesBWI76_RS15740 and BWI76_RS15745 overlap by 1 nucleotidesBWI76_RS15745 and BWI76_RS15750 overlap by 4 nucleotidesBWI76_RS15750 and BWI76_RS15755 are separated by 20 nucleotidesBWI76_RS15755 and BWI76_RS15760 overlap by 4 nucleotidesBWI76_RS15760 and BWI76_RS15765 overlap by 17 nucleotides BWI76_RS15730: BWI76_RS15730 - hypothetical protein, at 3,280,237 to 3,280,467 _RS15730 BWI76_RS15735: BWI76_RS15735 - hypothetical protein, at 3,280,460 to 3,281,005 _RS15735 BWI76_RS15740: BWI76_RS15740 - HNH endonuclease, at 3,281,002 to 3,281,382 _RS15740 BWI76_RS15745: BWI76_RS15745 - hypothetical protein, at 3,281,382 to 3,282,116 _RS15745 BWI76_RS15750: BWI76_RS15750 - hypothetical protein, at 3,282,113 to 3,282,430 _RS15750 BWI76_RS15755: BWI76_RS15755 - hypothetical protein, at 3,282,451 to 3,282,711 _RS15755 BWI76_RS15760: BWI76_RS15760 - hypothetical protein, at 3,282,708 to 3,282,899 _RS15760 BWI76_RS15765: BWI76_RS15765 - hypothetical protein, at 3,282,883 to 3,283,599 _RS15765 Position (kb) 3281 3282 3283Strain fitness (log2 ratio) -2 -1 0 1at 3280.459 kb on - strandat 3280.712 kb on + strand, within BWI76_RS15735at 3280.717 kb on - strand, within BWI76_RS15735at 3280.976 kb on - strandat 3281.124 kb on - strand, within BWI76_RS15740at 3281.236 kb on + strand, within BWI76_RS15740at 3281.238 kb on + strand, within BWI76_RS15740at 3281.520 kb on - strand, within BWI76_RS15745at 3281.692 kb on + strand, within BWI76_RS15745at 3281.700 kb on - strand, within BWI76_RS15745at 3281.917 kb on + strand, within BWI76_RS15745at 3281.925 kb on - strand, within BWI76_RS15745at 3282.080 kb on + strandat 3282.127 kb on + strandat 3282.163 kb on + strand, within BWI76_RS15750at 3282.548 kb on - strand, within BWI76_RS15755at 3282.604 kb on - strand, within BWI76_RS15755at 3282.604 kb on - strand, within BWI76_RS15755at 3282.604 kb on - strand, within BWI76_RS15755at 3282.604 kb on - strand, within BWI76_RS15755at 3282.606 kb on - strand, within BWI76_RS15755at 3282.654 kb on + strand, within BWI76_RS15755at 3282.687 kb on + strandat 3282.729 kb on - strand, within BWI76_RS15760at 3282.890 kb on + strandat 3282.928 kb on - strandat 3283.036 kb on - strand, within BWI76_RS15765

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x
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3,280,459 - +0.2
3,280,712 + BWI76_RS15735 0.46 -0.4
3,280,717 - BWI76_RS15735 0.47 -0.2
3,280,976 - -0.4
3,281,124 - BWI76_RS15740 0.32 -1.1
3,281,236 + BWI76_RS15740 0.61 -1.4
3,281,238 + BWI76_RS15740 0.62 +0.2
3,281,520 - BWI76_RS15745 0.19 -0.1
3,281,692 + BWI76_RS15745 0.42 -1.1
3,281,700 - BWI76_RS15745 0.43 -1.4
3,281,917 + BWI76_RS15745 0.73 -2.1
3,281,925 - BWI76_RS15745 0.74 -1.9
3,282,080 + +0.6
3,282,127 + +0.0
3,282,163 + BWI76_RS15750 0.16 -0.2
3,282,548 - BWI76_RS15755 0.37 +0.8
3,282,604 - BWI76_RS15755 0.59 +0.9
3,282,604 - BWI76_RS15755 0.59 -0.1
3,282,604 - BWI76_RS15755 0.59 -0.3
3,282,604 - BWI76_RS15755 0.59 +0.7
3,282,606 - BWI76_RS15755 0.59 -0.2
3,282,654 + BWI76_RS15755 0.78 +0.1
3,282,687 + +0.1
3,282,729 - BWI76_RS15760 0.11 +0.0
3,282,890 + +1.3
3,282,928 - +1.0
3,283,036 - BWI76_RS15765 0.21 -0.6

Or see this region's nucleotide sequence