Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13395 and BWI76_RS13400 are separated by 326 nucleotides BWI76_RS13400 and BWI76_RS13405 are separated by 365 nucleotides
BWI76_RS13395: BWI76_RS13395 - cytochrome D ubiquinol oxidase subunit I, at 2,792,601 to 2,793,998
_RS13395
BWI76_RS13400: BWI76_RS13400 - GntR family transcriptional regulator, at 2,794,325 to 2,795,737
_RS13400
BWI76_RS13405: BWI76_RS13405 - spermidine/putrescine ABC transporter substrate-binding protein, at 2,796,103 to 2,797,248
_RS13405
Position (kb)
2794
2795
2796 Strain fitness (log2 ratio)
-1
0
1 at 2793.425 kb on - strand, within BWI76_RS13395 at 2793.509 kb on - strand, within BWI76_RS13395 at 2793.623 kb on - strand, within BWI76_RS13395 at 2793.650 kb on + strand, within BWI76_RS13395 at 2794.031 kb on + strand at 2794.147 kb on - strand at 2794.252 kb on - strand at 2794.381 kb on + strand at 2794.722 kb on - strand, within BWI76_RS13400 at 2795.088 kb on + strand, within BWI76_RS13400 at 2795.096 kb on - strand, within BWI76_RS13400 at 2795.389 kb on + strand, within BWI76_RS13400 at 2795.401 kb on + strand, within BWI76_RS13400 at 2795.601 kb on - strand at 2795.663 kb on + strand at 2795.736 kb on + strand at 2795.751 kb on + strand at 2795.756 kb on + strand at 2795.772 kb on + strand at 2795.783 kb on + strand at 2796.023 kb on - strand at 2796.092 kb on + strand at 2796.141 kb on + strand at 2796.362 kb on + strand, within BWI76_RS13405 at 2796.370 kb on - strand, within BWI76_RS13405 at 2796.510 kb on + strand, within BWI76_RS13405
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 2,793,425 - BWI76_RS13395 0.59 -1.2 2,793,509 - BWI76_RS13395 0.65 +0.4 2,793,623 - BWI76_RS13395 0.73 +0.2 2,793,650 + BWI76_RS13395 0.75 -1.0 2,794,031 + +0.0 2,794,147 - -0.6 2,794,252 - +0.6 2,794,381 + +0.0 2,794,722 - BWI76_RS13400 0.28 +1.5 2,795,088 + BWI76_RS13400 0.54 +0.6 2,795,096 - BWI76_RS13400 0.55 +0.3 2,795,389 + BWI76_RS13400 0.75 -0.2 2,795,401 + BWI76_RS13400 0.76 -0.1 2,795,601 - +0.1 2,795,663 + -0.0 2,795,736 + +0.1 2,795,751 + -0.6 2,795,756 + +0.0 2,795,772 + -1.2 2,795,783 + -0.2 2,796,023 - +0.7 2,796,092 + +0.8 2,796,141 + +0.2 2,796,362 + BWI76_RS13405 0.23 -0.5 2,796,370 - BWI76_RS13405 0.23 -1.5 2,796,510 + BWI76_RS13405 0.36 -0.3
Or see this region's nucleotide sequence