Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01925

Experiment: RCH2_defined_glucose with MS_media 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01920 and BWI76_RS01925 are separated by 214 nucleotidesBWI76_RS01925 and BWI76_RS01930 are separated by 197 nucleotides BWI76_RS01920: BWI76_RS01920 - alanine racemase, at 391,002 to 392,081 _RS01920 BWI76_RS01925: BWI76_RS01925 - aromatic amino acid aminotransferase, at 392,296 to 393,489 _RS01925 BWI76_RS01930: BWI76_RS01930 - acid phosphatase/phosphotransferase, at 393,687 to 394,400 _RS01930 Position (kb) 392 393 394Strain fitness (log2 ratio) -2 -1 0 1at 391.301 kb on - strand, within BWI76_RS01920at 391.301 kb on - strand, within BWI76_RS01920at 391.417 kb on + strand, within BWI76_RS01920at 391.429 kb on + strand, within BWI76_RS01920at 391.439 kb on + strand, within BWI76_RS01920at 391.439 kb on + strand, within BWI76_RS01920at 391.447 kb on - strand, within BWI76_RS01920at 391.555 kb on + strand, within BWI76_RS01920at 391.590 kb on + strand, within BWI76_RS01920at 391.830 kb on + strand, within BWI76_RS01920at 392.257 kb on - strandat 392.267 kb on - strandat 392.508 kb on + strand, within BWI76_RS01925at 392.563 kb on - strand, within BWI76_RS01925at 392.596 kb on - strand, within BWI76_RS01925at 392.840 kb on + strand, within BWI76_RS01925at 392.860 kb on + strand, within BWI76_RS01925at 392.988 kb on - strand, within BWI76_RS01925at 393.187 kb on + strand, within BWI76_RS01925at 393.212 kb on - strand, within BWI76_RS01925at 393.263 kb on + strand, within BWI76_RS01925at 393.273 kb on + strand, within BWI76_RS01925at 393.280 kb on - strand, within BWI76_RS01925at 393.281 kb on + strand, within BWI76_RS01925at 393.369 kb on + strand, within BWI76_RS01925at 393.371 kb on - strandat 393.371 kb on - strandat 393.371 kb on - strandat 393.390 kb on + strandat 393.457 kb on - strandat 393.457 kb on - strandat 393.463 kb on + strandat 393.484 kb on + strandat 393.493 kb on - strandat 393.504 kb on + strandat 393.512 kb on - strandat 393.531 kb on + strandat 393.531 kb on + strandat 393.531 kb on + strandat 393.539 kb on - strandat 393.583 kb on - strandat 393.699 kb on + strandat 393.706 kb on + strandat 393.823 kb on - strand, within BWI76_RS01930at 393.823 kb on - strand, within BWI76_RS01930at 393.838 kb on - strand, within BWI76_RS01930at 394.227 kb on + strand, within BWI76_RS01930at 394.285 kb on + strand, within BWI76_RS01930at 394.369 kb on + strandat 394.377 kb on - strandat 394.473 kb on + strandat 394.488 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x
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391,301 - BWI76_RS01920 0.28 -0.3
391,301 - BWI76_RS01920 0.28 -0.4
391,417 + BWI76_RS01920 0.38 -0.3
391,429 + BWI76_RS01920 0.40 -0.5
391,439 + BWI76_RS01920 0.40 +0.6
391,439 + BWI76_RS01920 0.40 +0.8
391,447 - BWI76_RS01920 0.41 +1.6
391,555 + BWI76_RS01920 0.51 -0.2
391,590 + BWI76_RS01920 0.54 -0.2
391,830 + BWI76_RS01920 0.77 +1.4
392,257 - -0.1
392,267 - -1.1
392,508 + BWI76_RS01925 0.18 -0.2
392,563 - BWI76_RS01925 0.22 -1.1
392,596 - BWI76_RS01925 0.25 -1.9
392,840 + BWI76_RS01925 0.46 -0.7
392,860 + BWI76_RS01925 0.47 -0.9
392,988 - BWI76_RS01925 0.58 -1.2
393,187 + BWI76_RS01925 0.75 -0.7
393,212 - BWI76_RS01925 0.77 -0.4
393,263 + BWI76_RS01925 0.81 -0.7
393,273 + BWI76_RS01925 0.82 -0.2
393,280 - BWI76_RS01925 0.82 -0.6
393,281 + BWI76_RS01925 0.82 -1.9
393,369 + BWI76_RS01925 0.90 -1.3
393,371 - -1.1
393,371 - -0.6
393,371 - -0.1
393,390 + -0.4
393,457 - -1.1
393,457 - -1.6
393,463 + -0.4
393,484 + +0.4
393,493 - -1.0
393,504 + -0.2
393,512 - -1.6
393,531 + -0.2
393,531 + +0.6
393,531 + +0.0
393,539 - -0.2
393,583 - +0.1
393,699 + +0.2
393,706 + +1.2
393,823 - BWI76_RS01930 0.19 -0.5
393,823 - BWI76_RS01930 0.19 +0.2
393,838 - BWI76_RS01930 0.21 -0.2
394,227 + BWI76_RS01930 0.76 +0.0
394,285 + BWI76_RS01930 0.84 -0.1
394,369 + +0.0
394,377 - -0.3
394,473 + +0.6
394,488 - -0.6

Or see this region's nucleotide sequence