Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00370 and BWI76_RS00375 are separated by 3 nucleotides BWI76_RS00375 and BWI76_RS00380 are separated by 2 nucleotides BWI76_RS00380 and BWI76_RS00385 are separated by 258 nucleotides
BWI76_RS00370: BWI76_RS00370 - GTPase-activating protein, at 78,551 to 79,057
_RS00370
BWI76_RS00375: BWI76_RS00375 - hypothetical protein, at 79,061 to 79,243
_RS00375
BWI76_RS00380: BWI76_RS00380 - oxygen-independent coproporphyrinogen III oxidase, at 79,246 to 80,619
_RS00380
BWI76_RS00385: BWI76_RS00385 - nitrogen assimilation regulatory protein, at 80,878 to 82,287
_RS00385
Position (kb) 79
80
81 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 78.268 kb on - strand at 78.290 kb on + strand at 78.369 kb on - strand at 78.391 kb on - strand at 78.532 kb on + strand at 78.572 kb on - strand at 78.835 kb on - strand, within BWI76_RS00370 at 78.885 kb on + strand at 78.885 kb on - strand, within BWI76_RS00370 at 78.912 kb on + strand, within BWI76_RS00370 at 78.912 kb on + strand, within BWI76_RS00370 at 78.922 kb on + strand, within BWI76_RS00370 at 78.930 kb on - strand, within BWI76_RS00370 at 78.987 kb on - strand, within BWI76_RS00370 at 79.102 kb on + strand, within BWI76_RS00375 at 79.110 kb on - strand, within BWI76_RS00375 at 79.110 kb on - strand, within BWI76_RS00375 at 79.234 kb on + strand at 79.250 kb on - strand at 79.270 kb on + strand at 79.271 kb on + strand at 79.273 kb on - strand at 79.308 kb on + strand at 79.316 kb on - strand at 79.316 kb on - strand at 79.316 kb on - strand at 79.482 kb on + strand, within BWI76_RS00380 at 79.482 kb on + strand, within BWI76_RS00380 at 79.482 kb on + strand, within BWI76_RS00380 at 79.482 kb on + strand, within BWI76_RS00380 at 79.490 kb on - strand, within BWI76_RS00380 at 79.490 kb on - strand, within BWI76_RS00380 at 79.490 kb on - strand, within BWI76_RS00380 at 79.490 kb on - strand at 79.583 kb on - strand, within BWI76_RS00380 at 79.628 kb on - strand, within BWI76_RS00380 at 79.657 kb on - strand, within BWI76_RS00380 at 79.754 kb on - strand, within BWI76_RS00380 at 79.754 kb on - strand, within BWI76_RS00380 at 79.756 kb on - strand, within BWI76_RS00380 at 79.811 kb on - strand, within BWI76_RS00380 at 79.850 kb on + strand, within BWI76_RS00380 at 79.858 kb on - strand, within BWI76_RS00380 at 79.938 kb on + strand, within BWI76_RS00380 at 79.946 kb on - strand, within BWI76_RS00380 at 80.038 kb on - strand, within BWI76_RS00380 at 80.157 kb on - strand, within BWI76_RS00380 at 80.259 kb on + strand, within BWI76_RS00380 at 80.422 kb on + strand, within BWI76_RS00380 at 80.524 kb on + strand at 80.636 kb on + strand at 80.686 kb on - strand at 80.720 kb on - strand at 80.724 kb on + strand at 80.812 kb on - strand at 80.913 kb on + strand at 80.959 kb on + strand at 80.983 kb on + strand at 80.989 kb on - strand at 81.125 kb on + strand, within BWI76_RS00385 at 81.163 kb on + strand, within BWI76_RS00385 at 81.163 kb on + strand, within BWI76_RS00385 at 81.163 kb on + strand, within BWI76_RS00385 at 81.171 kb on - strand, within BWI76_RS00385 at 81.221 kb on - strand, within BWI76_RS00385 at 81.241 kb on + strand, within BWI76_RS00385 at 81.432 kb on + strand, within BWI76_RS00385 at 81.432 kb on + strand, within BWI76_RS00385 at 81.600 kb on + strand, within BWI76_RS00385
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 78,268 - +0.0 78,290 + -0.4 78,369 - -0.1 78,391 - +0.3 78,532 + +0.3 78,572 - -0.1 78,835 - BWI76_RS00370 0.56 +0.1 78,885 + -1.6 78,885 - BWI76_RS00370 0.66 -0.1 78,912 + BWI76_RS00370 0.71 -0.8 78,912 + BWI76_RS00370 0.71 +0.4 78,922 + BWI76_RS00370 0.73 -0.7 78,930 - BWI76_RS00370 0.75 -0.3 78,987 - BWI76_RS00370 0.86 +0.1 79,102 + BWI76_RS00375 0.22 +0.1 79,110 - BWI76_RS00375 0.27 -0.9 79,110 - BWI76_RS00375 0.27 -0.1 79,234 + -0.6 79,250 - -1.8 79,270 + -0.2 79,271 + -1.3 79,273 - +0.7 79,308 + -1.2 79,316 - -1.8 79,316 - -1.1 79,316 - -2.6 79,482 + BWI76_RS00380 0.17 -1.1 79,482 + BWI76_RS00380 0.17 -0.2 79,482 + BWI76_RS00380 0.17 -0.3 79,482 + BWI76_RS00380 0.17 -1.3 79,490 - BWI76_RS00380 0.18 -1.3 79,490 - BWI76_RS00380 0.18 -1.6 79,490 - BWI76_RS00380 0.18 -0.3 79,490 - +0.3 79,583 - BWI76_RS00380 0.25 -1.6 79,628 - BWI76_RS00380 0.28 -1.5 79,657 - BWI76_RS00380 0.30 -1.8 79,754 - BWI76_RS00380 0.37 -1.1 79,754 - BWI76_RS00380 0.37 -0.9 79,756 - BWI76_RS00380 0.37 -1.6 79,811 - BWI76_RS00380 0.41 -0.5 79,850 + BWI76_RS00380 0.44 -1.9 79,858 - BWI76_RS00380 0.45 -0.9 79,938 + BWI76_RS00380 0.50 -0.7 79,946 - BWI76_RS00380 0.51 -0.3 80,038 - BWI76_RS00380 0.58 -0.9 80,157 - BWI76_RS00380 0.66 -0.4 80,259 + BWI76_RS00380 0.74 -2.0 80,422 + BWI76_RS00380 0.86 -2.1 80,524 + -2.0 80,636 + -0.5 80,686 - -0.2 80,720 - -0.4 80,724 + -1.0 80,812 - +0.5 80,913 + -0.9 80,959 + -0.8 80,983 + -1.3 80,989 - -0.6 81,125 + BWI76_RS00385 0.18 -0.3 81,163 + BWI76_RS00385 0.20 -1.2 81,163 + BWI76_RS00385 0.20 -1.2 81,163 + BWI76_RS00385 0.20 -1.5 81,171 - BWI76_RS00385 0.21 -3.1 81,221 - BWI76_RS00385 0.24 -2.3 81,241 + BWI76_RS00385 0.26 -0.8 81,432 + BWI76_RS00385 0.39 -0.9 81,432 + BWI76_RS00385 0.39 -1.4 81,600 + BWI76_RS00385 0.51 -1.3
Or see this region's nucleotide sequence