Experiment: SBM - with RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aspC-2 and SO2407 are separated by 401 nucleotides SO2407 and SO2408 are separated by 22 nucleotides SO2408 and SO2409 overlap by 4 nucleotides
SO2406: aspC-2 - aspartate aminotransferase (NCBI ptt file), at 2,509,451 to 2,510,641
aspC-2
SO2407: SO2407 - conserved hypothetical protein (NCBI ptt file), at 2,511,043 to 2,512,602
SO2407
SO2408: SO2408 - radical activating enzyme (NCBI ptt file), at 2,512,625 to 2,513,485
SO2408
SO2409: SO2409 - transcriptional regulator, LysR family (NCBI ptt file), at 2,513,482 to 2,514,402
SO2409
Position (kb)
2511
2512
2513 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2510.064 kb on + strand, within aspC-2 at 2510.178 kb on - strand, within aspC-2 at 2510.275 kb on + strand, within aspC-2 at 2510.275 kb on + strand, within aspC-2 at 2510.275 kb on + strand, within aspC-2 at 2510.301 kb on - strand, within aspC-2 at 2510.370 kb on + strand, within aspC-2 at 2510.378 kb on - strand, within aspC-2 at 2510.388 kb on - strand, within aspC-2 at 2510.562 kb on + strand at 2510.572 kb on + strand at 2510.580 kb on - strand at 2510.815 kb on + strand at 2511.087 kb on + strand at 2511.095 kb on - strand at 2511.136 kb on + strand at 2511.212 kb on + strand, within SO2407 at 2511.212 kb on + strand, within SO2407 at 2511.220 kb on - strand, within SO2407 at 2512.099 kb on - strand, within SO2407 at 2512.233 kb on + strand, within SO2407 at 2512.253 kb on - strand, within SO2407 at 2512.298 kb on - strand, within SO2407 at 2512.368 kb on + strand, within SO2407 at 2512.526 kb on - strand at 2512.824 kb on - strand, within SO2408 at 2512.852 kb on + strand, within SO2408 at 2512.898 kb on - strand, within SO2408 at 2512.962 kb on + strand, within SO2408 at 2512.967 kb on + strand, within SO2408 at 2513.135 kb on - strand, within SO2408 at 2513.135 kb on - strand, within SO2408 at 2513.170 kb on - strand, within SO2408 at 2513.295 kb on - strand, within SO2408 at 2513.441 kb on - strand at 2513.441 kb on - strand at 2513.489 kb on + strand at 2513.532 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SBM - with RNA protect remove 2,510,064 + aspC-2 SO2406 0.51 -0.7 2,510,178 - aspC-2 SO2406 0.61 +0.4 2,510,275 + aspC-2 SO2406 0.69 -1.6 2,510,275 + aspC-2 SO2406 0.69 -1.7 2,510,275 + aspC-2 SO2406 0.69 -2.0 2,510,301 - aspC-2 SO2406 0.71 -1.8 2,510,370 + aspC-2 SO2406 0.77 -1.5 2,510,378 - aspC-2 SO2406 0.78 -1.2 2,510,388 - aspC-2 SO2406 0.79 -0.7 2,510,562 + -2.1 2,510,572 + -0.2 2,510,580 - -2.2 2,510,815 + +0.2 2,511,087 + -1.0 2,511,095 - +0.2 2,511,136 + -1.0 2,511,212 + SO2407 0.11 -0.1 2,511,212 + SO2407 0.11 +1.2 2,511,220 - SO2407 0.11 -0.2 2,512,099 - SO2407 0.68 +1.4 2,512,233 + SO2407 0.76 +2.0 2,512,253 - SO2407 0.78 +0.7 2,512,298 - SO2407 0.80 +0.0 2,512,368 + SO2407 0.85 -0.7 2,512,526 - -0.6 2,512,824 - SO2408 0.23 -0.3 2,512,852 + SO2408 0.26 -0.7 2,512,898 - SO2408 0.32 -0.2 2,512,962 + SO2408 0.39 +1.6 2,512,967 + SO2408 0.40 -0.2 2,513,135 - SO2408 0.59 -0.8 2,513,135 - SO2408 0.59 -0.3 2,513,170 - SO2408 0.63 +1.7 2,513,295 - SO2408 0.78 +0.9 2,513,441 - -0.7 2,513,441 - -0.6 2,513,489 + -0.7 2,513,532 + +0.1
Or see this region's nucleotide sequence