Experiment: SBM - with RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1258 and SO1259 are separated by 135 nucleotides SO1259 and SO1260 are separated by 22 nucleotides
SO1258: SO1258 - adenylosuccinate synthetase, putative (NCBI ptt file), at 1,306,434 to 1,307,690
SO1258
SO1259: SO1259 - transcriptional regulator, LysR family (NCBI ptt file), at 1,307,826 to 1,308,731
SO1259
SO1260: SO1260 - hypothetical amino acid ABC transporter (NCBI ptt file), at 1,308,754 to 1,309,575
SO1260
Position (kb)
1307
1308
1309 Strain fitness (log2 ratio)
-2
-1
0
1 at 1306.938 kb on + strand, within SO1258 at 1306.938 kb on + strand, within SO1258 at 1306.938 kb on + strand, within SO1258 at 1306.938 kb on + strand, within SO1258 at 1306.938 kb on + strand, within SO1258 at 1306.946 kb on - strand, within SO1258 at 1306.946 kb on - strand, within SO1258 at 1306.946 kb on - strand, within SO1258 at 1307.070 kb on - strand, within SO1258 at 1307.150 kb on + strand, within SO1258 at 1307.167 kb on + strand, within SO1258 at 1307.284 kb on + strand, within SO1258 at 1307.301 kb on + strand, within SO1258 at 1307.364 kb on + strand, within SO1258 at 1307.374 kb on + strand, within SO1258 at 1307.379 kb on + strand, within SO1258 at 1307.379 kb on + strand, within SO1258 at 1307.379 kb on + strand, within SO1258 at 1307.379 kb on + strand, within SO1258 at 1307.379 kb on + strand, within SO1258 at 1307.379 kb on + strand, within SO1258 at 1307.382 kb on - strand, within SO1258 at 1307.384 kb on + strand, within SO1258 at 1307.384 kb on + strand, within SO1258 at 1307.537 kb on + strand, within SO1258 at 1307.687 kb on + strand at 1307.903 kb on + strand at 1307.903 kb on + strand at 1307.903 kb on + strand at 1307.903 kb on + strand at 1307.911 kb on - strand at 1307.911 kb on - strand at 1307.911 kb on - strand at 1307.911 kb on - strand at 1307.911 kb on - strand at 1307.911 kb on - strand at 1307.913 kb on - strand at 1307.965 kb on + strand, within SO1259 at 1307.989 kb on + strand, within SO1259 at 1307.997 kb on - strand, within SO1259 at 1308.053 kb on - strand, within SO1259 at 1308.097 kb on + strand, within SO1259 at 1308.111 kb on - strand, within SO1259 at 1308.111 kb on - strand, within SO1259 at 1308.129 kb on + strand, within SO1259 at 1308.217 kb on + strand, within SO1259 at 1308.217 kb on + strand, within SO1259 at 1308.217 kb on + strand, within SO1259 at 1308.217 kb on + strand, within SO1259 at 1308.217 kb on + strand, within SO1259 at 1308.225 kb on - strand, within SO1259 at 1308.225 kb on - strand, within SO1259 at 1308.317 kb on - strand, within SO1259 at 1308.444 kb on + strand, within SO1259 at 1308.444 kb on + strand, within SO1259 at 1308.452 kb on - strand, within SO1259 at 1308.452 kb on - strand, within SO1259 at 1308.493 kb on + strand, within SO1259 at 1308.808 kb on + strand at 1308.808 kb on + strand at 1308.816 kb on - strand at 1308.816 kb on - strand at 1308.874 kb on - strand, within SO1260 at 1309.188 kb on - strand, within SO1260 at 1309.293 kb on - strand, within SO1260 at 1309.425 kb on + strand, within SO1260 at 1309.539 kb on + strand at 1309.595 kb on + strand at 1309.709 kb on - strand at 1309.714 kb on + strand at 1309.722 kb on + strand at 1309.722 kb on + strand at 1309.722 kb on + strand at 1309.730 kb on + strand at 1309.730 kb on - strand at 1309.730 kb on - strand at 1309.730 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SBM - with RNA protect remove 1,306,938 + SO1258 0.40 +1.1 1,306,938 + SO1258 0.40 -2.2 1,306,938 + SO1258 0.40 +0.9 1,306,938 + SO1258 0.40 -0.7 1,306,938 + SO1258 0.40 -1.1 1,306,946 - SO1258 0.41 -0.5 1,306,946 - SO1258 0.41 +0.3 1,306,946 - SO1258 0.41 -0.4 1,307,070 - SO1258 0.51 -0.3 1,307,150 + SO1258 0.57 +0.4 1,307,167 + SO1258 0.58 +1.2 1,307,284 + SO1258 0.68 -1.2 1,307,301 + SO1258 0.69 -1.4 1,307,364 + SO1258 0.74 +0.3 1,307,374 + SO1258 0.75 +0.6 1,307,379 + SO1258 0.75 +1.2 1,307,379 + SO1258 0.75 -1.1 1,307,379 + SO1258 0.75 -0.8 1,307,379 + SO1258 0.75 +0.6 1,307,379 + SO1258 0.75 -0.2 1,307,379 + SO1258 0.75 +0.4 1,307,382 - SO1258 0.75 -0.8 1,307,384 + SO1258 0.76 +0.2 1,307,384 + SO1258 0.76 -0.5 1,307,537 + SO1258 0.88 +0.0 1,307,687 + +0.4 1,307,903 + -0.5 1,307,903 + +0.3 1,307,903 + +0.4 1,307,903 + +1.3 1,307,911 - -1.1 1,307,911 - -0.8 1,307,911 - -0.4 1,307,911 - +0.0 1,307,911 - -0.2 1,307,911 - -0.5 1,307,913 - +0.9 1,307,965 + SO1259 0.15 +0.2 1,307,989 + SO1259 0.18 -0.6 1,307,997 - SO1259 0.19 +0.2 1,308,053 - SO1259 0.25 +0.1 1,308,097 + SO1259 0.30 -0.4 1,308,111 - SO1259 0.31 -0.6 1,308,111 - SO1259 0.31 +0.5 1,308,129 + SO1259 0.33 -0.2 1,308,217 + SO1259 0.43 -0.3 1,308,217 + SO1259 0.43 -0.4 1,308,217 + SO1259 0.43 +0.3 1,308,217 + SO1259 0.43 +0.2 1,308,217 + SO1259 0.43 -0.4 1,308,225 - SO1259 0.44 -1.0 1,308,225 - SO1259 0.44 -0.3 1,308,317 - SO1259 0.54 -0.6 1,308,444 + SO1259 0.68 +0.3 1,308,444 + SO1259 0.68 +0.8 1,308,452 - SO1259 0.69 -0.5 1,308,452 - SO1259 0.69 -0.3 1,308,493 + SO1259 0.74 -0.2 1,308,808 + -0.9 1,308,808 + -0.5 1,308,816 - +0.2 1,308,816 - -0.9 1,308,874 - SO1260 0.15 -0.9 1,309,188 - SO1260 0.53 +0.1 1,309,293 - SO1260 0.66 -0.9 1,309,425 + SO1260 0.82 +0.8 1,309,539 + +0.7 1,309,595 + -1.2 1,309,709 - -1.2 1,309,714 + -1.8 1,309,722 + +1.2 1,309,722 + +0.0 1,309,722 + -1.6 1,309,730 + +1.3 1,309,730 - -0.6 1,309,730 - -0.1 1,309,730 - +0.9
Or see this region's nucleotide sequence