Experiment: SBM - with RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gapA-1 and SO0539 are separated by 9 nucleotides SO0539 and SO0540 are separated by 106 nucleotides
SO0538: gapA-1 - glyceraldehyde 3-phosphate dehydrogenase (NCBI ptt file), at 557,305 to 558,318
gapA-1
SO0539: SO0539 - transporter, putative (NCBI ptt file), at 558,328 to 559,590
SO0539
SO0540: SO0540 - conserved hypothetical protein (NCBI ptt file), at 559,697 to 560,500
SO0540
Position (kb) 558
559
560 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 557.348 kb on + strand at 557.348 kb on + strand at 557.348 kb on + strand at 557.348 kb on + strand at 557.348 kb on + strand at 557.356 kb on - strand at 557.356 kb on - strand at 557.356 kb on - strand at 557.356 kb on - strand at 557.356 kb on - strand at 557.356 kb on - strand at 557.358 kb on + strand at 557.477 kb on - strand, within gapA-1 at 557.477 kb on - strand, within gapA-1 at 557.543 kb on - strand, within gapA-1 at 557.574 kb on - strand, within gapA-1 at 557.695 kb on + strand, within gapA-1 at 557.884 kb on + strand, within gapA-1 at 557.884 kb on + strand, within gapA-1 at 557.884 kb on + strand, within gapA-1 at 557.884 kb on + strand, within gapA-1 at 557.884 kb on + strand, within gapA-1 at 557.892 kb on - strand, within gapA-1 at 557.892 kb on - strand, within gapA-1 at 557.892 kb on - strand, within gapA-1 at 557.915 kb on + strand, within gapA-1 at 557.923 kb on - strand, within gapA-1 at 557.971 kb on - strand, within gapA-1 at 557.982 kb on + strand, within gapA-1 at 557.990 kb on - strand, within gapA-1 at 558.012 kb on + strand, within gapA-1 at 558.110 kb on - strand, within gapA-1 at 558.215 kb on - strand, within gapA-1 at 558.352 kb on - strand at 558.436 kb on + strand at 558.601 kb on - strand, within SO0539 at 558.650 kb on - strand, within SO0539 at 558.675 kb on + strand, within SO0539 at 558.756 kb on + strand, within SO0539 at 558.784 kb on - strand, within SO0539 at 558.798 kb on + strand, within SO0539 at 558.826 kb on - strand, within SO0539 at 558.921 kb on + strand, within SO0539 at 558.934 kb on - strand, within SO0539 at 558.934 kb on - strand, within SO0539 at 558.934 kb on - strand, within SO0539 at 559.115 kb on - strand, within SO0539 at 559.159 kb on + strand, within SO0539 at 559.167 kb on - strand, within SO0539 at 559.167 kb on - strand, within SO0539 at 559.230 kb on - strand, within SO0539 at 559.331 kb on + strand, within SO0539 at 559.331 kb on + strand, within SO0539 at 559.331 kb on + strand, within SO0539 at 559.339 kb on - strand, within SO0539 at 559.403 kb on + strand, within SO0539 at 559.418 kb on + strand, within SO0539 at 559.469 kb on + strand at 559.469 kb on + strand at 559.602 kb on - strand at 559.624 kb on - strand at 559.635 kb on - strand at 559.655 kb on + strand at 559.655 kb on + strand at 559.656 kb on + strand at 559.663 kb on - strand at 559.663 kb on - strand at 559.673 kb on + strand at 559.706 kb on + strand at 560.024 kb on + strand, within SO0540 at 560.112 kb on + strand, within SO0540 at 560.112 kb on + strand, within SO0540 at 560.119 kb on + strand, within SO0540 at 560.281 kb on - strand, within SO0540 at 560.372 kb on + strand, within SO0540 at 560.451 kb on - strand at 560.453 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SBM - with RNA protect remove 557,348 + +0.5 557,348 + -0.2 557,348 + +1.1 557,348 + +0.4 557,348 + -0.6 557,356 - +0.3 557,356 - +3.2 557,356 - -0.2 557,356 - +1.0 557,356 - +0.1 557,356 - +0.5 557,358 + +0.1 557,477 - gapA-1 SO0538 0.17 -1.6 557,477 - gapA-1 SO0538 0.17 -1.0 557,543 - gapA-1 SO0538 0.23 +0.2 557,574 - gapA-1 SO0538 0.27 +0.6 557,695 + gapA-1 SO0538 0.38 -0.3 557,884 + gapA-1 SO0538 0.57 +0.2 557,884 + gapA-1 SO0538 0.57 +0.5 557,884 + gapA-1 SO0538 0.57 -0.7 557,884 + gapA-1 SO0538 0.57 +1.4 557,884 + gapA-1 SO0538 0.57 -0.2 557,892 - gapA-1 SO0538 0.58 +0.9 557,892 - gapA-1 SO0538 0.58 -0.1 557,892 - gapA-1 SO0538 0.58 +0.6 557,915 + gapA-1 SO0538 0.60 +0.2 557,923 - gapA-1 SO0538 0.61 +0.2 557,971 - gapA-1 SO0538 0.66 -1.6 557,982 + gapA-1 SO0538 0.67 -0.4 557,990 - gapA-1 SO0538 0.68 +0.2 558,012 + gapA-1 SO0538 0.70 -0.3 558,110 - gapA-1 SO0538 0.79 +0.1 558,215 - gapA-1 SO0538 0.90 +1.6 558,352 - +1.6 558,436 + -0.7 558,601 - SO0539 0.22 -0.7 558,650 - SO0539 0.25 +0.9 558,675 + SO0539 0.27 -0.9 558,756 + SO0539 0.34 -1.9 558,784 - SO0539 0.36 +0.2 558,798 + SO0539 0.37 +0.3 558,826 - SO0539 0.39 +0.5 558,921 + SO0539 0.47 +0.4 558,934 - SO0539 0.48 +0.1 558,934 - SO0539 0.48 -1.7 558,934 - SO0539 0.48 +0.1 559,115 - SO0539 0.62 -0.1 559,159 + SO0539 0.66 -1.0 559,167 - SO0539 0.66 +0.2 559,167 - SO0539 0.66 -0.4 559,230 - SO0539 0.71 -0.3 559,331 + SO0539 0.79 +0.7 559,331 + SO0539 0.79 +0.8 559,331 + SO0539 0.79 -0.4 559,339 - SO0539 0.80 -1.1 559,403 + SO0539 0.85 -0.2 559,418 + SO0539 0.86 -3.7 559,469 + -0.2 559,469 + +1.0 559,602 - -0.3 559,624 - +1.6 559,635 - +0.5 559,655 + -0.7 559,655 + +0.2 559,656 + -0.4 559,663 - -1.0 559,663 - -0.7 559,673 + +0.2 559,706 + +0.4 560,024 + SO0540 0.41 +0.1 560,112 + SO0540 0.52 +0.1 560,112 + SO0540 0.52 +0.2 560,119 + SO0540 0.52 +0.9 560,281 - SO0540 0.73 +0.2 560,372 + SO0540 0.84 -0.3 560,451 - -1.9 560,453 + +0.4
Or see this region's nucleotide sequence